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Volume 47, Issue 5
Displaying 1-6 of 6 articles from this issue
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PARTICULARLY ON THE EFFECT OF VARIOUS MEDIA ADDEDTakeji Takamura1956 Volume 47 Issue 5 Pages 271-284
Published: 1956
Released on J-STAGE: July 23, 2010
JOURNAL FREE ACCESSUsing the Warburg's gas manometric method the author measured the amount of oxygen gas used up for the respiration of gonococcus. In this procedure using three strains of gonococci, 2, 8cc of broth in the main compartment of flask, 0.2cc of 20% KOH in the centre well and 10mg of gonococci cultured at 36.5°C for 24 hours which was suspended in 0.2cc of Ringer's solution in sidearm were shaken for 15 minutes (turned 90 times per min). Thereafter gonococcal suspension was flowed into the main compartment to measure the amount of gas used from one hour (measured every 10 minutes) to four hours.
Then the maximal amount of oxygen gas consumption of gonococci cultured in various kinds of medium was measured as optimum.
In regard to peptone, the medium added with Proteose Peptone No. 3 Difco by 3%, and Teruuchi's peptone by 2% respectively were equally the best, and the liver extract is better than the beef extract.
Human bloods (whole blood, blood cells and serum), animal bloods (horse, goat, rabbit and guinea pig), saccharides (glucose, saccharose, raffinose, starch, fructose and xylose), amino acids (1-cystine, 1-methionine and 1-glutamic acid) and yeast extract were used as adding substances to medium. Concerning the human blood the medium added with the whole blood by 40% being kept warm at 56°C for 30min, was the best in these series of experiments with blood cells by 40% and warm blood serum in the same ratio.
The human and horse bloods were rather better than the others, and the horse blood was slightly better than the human blood.
The glucose added by 0.5% was the best, the amino acid particularly 1-eystine by 0.05%. and Difco yeast extract by 1% were the optimal dosis respectively.
It would seem preferable to use the culture medium with these substances in each optimal dosis in view of the respiration of gonococci as compared with GC medium. Eiken being mostly used at the present time.View full abstractDownload PDF (1759K) -
Shuji Ishiyama1956 Volume 47 Issue 5 Pages 285-314
Published: 1956
Released on J-STAGE: July 23, 2010
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Shûsaku Mizoguti, Shûzi Ôya, Shôiti Katumata1956 Volume 47 Issue 5 Pages 315-322
Published: 1956
Released on J-STAGE: July 23, 2010
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G. Momose, M. Hiraoka, S. Okamoto1956 Volume 47 Issue 5 Pages 323-329
Published: 1956
Released on J-STAGE: July 23, 2010
JOURNAL FREE ACCESSWe have reported four cases of primary carcinoma of the female urethra, and the problems involving pathologic change, predisposing factors, method of treatment, metastasis and prognosis have been discussed.View full abstractDownload PDF (2705K) -
THE V REPORT, CHANGES OF LEUCOCYTES AND SERUM PHOSPHATASE IN PERIPHERAL BLOOD OF RABBITS CAUSED BY INTRARENAL INJECTION OF TUBERCLE BACILLITatsuyoshi Arao1956 Volume 47 Issue 5 Pages 330-338
Published: 1956
Released on J-STAGE: July 23, 2010
JOURNAL FREE ACCESSFive mg of tubercle bacilli (Frankfurt human type) were injected into renal parenchym (left side only) of normal adult male rabbits and of rabbits sensibilized with BCG. Then the changes in acid and alkaline phosphatase reactions of neutrophilic leucocytes in peripheral blood smears were followed by the method of Takeuchi et al. at pH 5.6 and pH 9.3. The acid and alkaline phosphatase activity of the serum in these rabbits were also determined by Bodansky modified method at pH 5.6 and pH 9.3.
1) After the injection of tubercle bacilli into kidney, the phosphatase reaction increased in general. The increase was more rapid in rabbits sensitized with BCG, than in normal rabbits.
2) After a removal of affected kidney, the phosphatase reaction of leucocytes increased temporarily.
3) In rabbits which had experienced the infusion of tubercle bacilli, the phosphatase reaction of leucocytes decreased gradually from six to seven weeks after the injection of the bacilli into kidney.
4) The changes of the phosphatase activity in these rabbits were slight.
5) In the histopathological studies of these rabbits, the serious tuberculous changes were observed in the kidneys injected with tubercle bacilli.
6) From the results, it can be said that the phosphatase reaction of leucocytes in peripheral blood smears may indicate the nature of the tuberculous lesions.View full abstractDownload PDF (1699K) -
A STUDY ON NORMAL FEMALESKo Sakurai1956 Volume 47 Issue 5 Pages 339-347
Published: 1956
Released on J-STAGE: July 23, 2010
JOURNAL FREE ACCESSThe lateral roentgenograms of the bladder, urethra, uterus and vagina in 34 normal women, 20 to 55 years of age, were taken under the same condition.
In order to make geometrical measurements of the roentgenograms taken under strictly the same position, I believe that it is most rational to consider the anatomical conjugation (conjugata anatomica) as the baseline.
1. The bladder roentgenogram shows that the rate of enlargement of the anterior, or abdominal, wall of the bladder, is larger than that of the posterior, or uterovesical, wall depending on the position and condition.
2. The position of the external (urethral) meatus can be located by the lateral roentgenogram as follows: take a point on the line extending the anatomical conjugation 4.6±3.6mm inferiorly from the superior border of the symphysis pubis, and from there drop a vertical line 69.7±2.1mm posteriorly toward the sacrum. The end of the vertical line shows the external meatus.
3. The position of the internal meatus can be located by the lateral roentgenogram as follows: take a point on the anatomical conjugation 26.7±2.1mm from the superior border of symphysis toward sacral promontory and drop a vertical line 46.1±1.8mm posteriorly toward the sacrum. The end of the vertical line indecates the internal meatus. The measurements reported above are considered average for the Japanese women.
4. The position of the external meatus approaches the vaginal orifice with age, and the internal urethra has a tendency to occupy a more inferior position toward the outer genitalia.
5. The external and the internal meatuses vary their position with change of body position and with pressure. The range of mobility of the internal meatus in a vertical direction toward the anatomical conjugation, i.e. in a sagittal direction to the body, is greater than that of the external meatus. The range of mobility of the external meatus in a horizontal direction to the anatomical conjugation i.e. in an upper and lower direction to the body, is greater than that of the internal meatus.View full abstractDownload PDF (3959K)
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