Transperitoneal implantation of MH 134 hepatoma, Yoshida sarcoma and AH 130 hepatoma into the urinary bladder was performed in 57 rats and the spreading ways of the implanted tumor were examined. The results are as follows:
1) Metastasis were found in all cases. The growth of the metastasis was differed, according to the brands of implanted tumor cells as well as the experimental methods, although Yoshida sarcoma always showed pronounced metastasis.
2) In the early stage, spreading ways to the various organs were noted to be different. In the retroperitoneal lymph nodes, metastasis appeared in the cortical lymphatic sinus, especially in the marginal sinus. The internal genitalia were infiltrated directly from the bladder. In the liver, spleen and lung, metastasis developed chiefly in the vicinity of the blood vessels. In the kidney, both the renal calyces and the connective tissue surrounding the blood vessels in the medulla were involved. These different sites of the metastasis suggested the possibility of both hematogeneous and intracanalicular spread to the kidneys.
In the adrenal glands, stomach, intestine and pancreas, dissemination of the tumor cells took place from the ascites.
3) The most common sites of metastasis was the retroperitoneal lymphnodes. The lung, liver and kidneys followed next. The internal genitalia were less commonly involved. In the spleen, pancreas and adrenal glands, incidence of metastasis was differed in different brands of the implanted tumors. The adrenal glands were involved rarely, and involvement of both the stomach and intestine was further rare.
4) With lapse of time, the involved organs were increased in number and the metastasis became pronounced.
In 7 days after the implantation metastasis was found in both the lung and retroperitoneal lymphnodes. In 14 days the kidney, liver and spleen were also involved, in addition to the above mentioned organs.
In the fatal cases, died during this period, numerous sites of metastasis were found in the internal genitalia, stomach, intestine and pancreas.
In early stage, the tumor spread lymphogenously to the retroperitoneal lymph nodes and then, hematogenously to the lung. With lapse of time, blood borne metastasis appeared in the kidney, liver and spleen. Later, in the advanced stage intracanalicular metastasis occurred to the kidney and continuous extention of the tumor infiltration from the urinary bladder to the internal genitalia. The abdominal organs, such as the adrenal glands, stomach, intestine and pancreas, were invaded through the serosa by the tumor cell presenting in the ascites.

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It is widely assumed that the dysfunction of the urinary bladder seen in the diabetes mellitus is of neurogenic origin. However, no report has, so far, been issued which confirms this concept pathohistologically. In the preceeding reports, we reported that in the dogs with alloxane diabetes complicating the vesicl dysfunction, degeneration of the myelinated fibers was seen in the nerves innervating the urinary bladder, and suggested that the degeneration could be a main causative factor of the dysfunction.
In the present study the degeneration in the lumbar and sacral region of the spinal cord stained by Marchi's method was examined histologically in 8 dogs with alloxane diabetes complicating the vesical dysfunction.
The results are summarized as follows:
1) Presence of degenerationin the spinal cord was confirmed in 6 dogs; pronounced degeneration in 4 dog and slight one in 2 dogs.
2) The sites of the degeneration were found throughout the lumbar and sacral region of the spinal cord in the dorsal horn, intradural part of the posterior root, intermediate zone of the gray matter, lateral horn, lateral fasciculus, ventral horn, intradural part of the ventral root and most commonly in the dorsal fasciculus. The degeneration of the dorsal fasciculus, diffuse but confined in a area, was seen only in the lumbar region, especially in its upper part. No notable relation was established between the duration of diabetes and severity or sites of the degeneration in the spinal cord.
3) The degeneration was found in the spinal cord in most dogs complicated with the vesical dysfunction, while in dogs without the vesical dysfunction the degeneration was also found, although it was in a mild degree.
If follows from these results that diabetes mellitus develops the degeneration of the central nervous system, and that the degeneration in the spinal cord is seen in most cases complicated with the vesical dysfunction.

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Acid-base equilibrium and renal function were observed on the cases of ureterosigmoidostomy, sigmoidocutaneus ureterostomy (transplantation of one ureter to sigmoid colon and one ureter to skin), ileocystoplasty, uretero-ileo-cystoneostomy, and some other conditions. The studies of sigmoidal absorption of urine and ammonium chloride solutions were made on the dogs, and possible causes for electrolyte imbalance were discussed.
1) The development of hyperchloremic acidosis was related both to the types of operation and the renal function.
2) Polyuria and low urine electrolyte concentrations were usually obsurved in the cases of ileocystoplasty and ureterostomy in acidosis. The urine of such cases showed an impaired ability to excrete hydrogen ions and ammonia and to reabsorb bicarbonate.
3) In the cases of ileocystoplasty the ileal urine contained more sodium and bicarbonate, less pottasium, chloride and urea than the renal urine.
4) The instillation of hypotonic ammonium chloride solution into dog's sigmoid effected a decrease in blood chloride levels, while hypertonic ammonium chloide solution effected an increase.
5) In the cases of urinary diversions acidosis may occur when renal function is involved and the kidney is no longer able to eliminate acid loads to the body. The sites of involvement are mainly renal tubles, and the patients are in the tendency to be dehydrated on account of continuous loss of water and elecyrolytes. Reabsorption of urinary constituents and alkali loss from the bowel increase acid loads and accelerate dehydration. The high blood chloride levels would be due to a compensatory mechanism, whereby the total anion (hence cation) concentration is maintained relatively constant.
6) For the treatment of hyperchloremic acidosis sodium bicarbonate is remarkably effective.
7) The 3 year and 5 year survival rates of intestinal urinary diversions were 20.0 per cent and 9.1 per cent for ureterosigmoidostomy, 50.0 per cent and 37.5 per cent for sigmoidocutaneus ureterostomy, 66.7 per cent and 60.0 per cent for ileocystoplasty, and 80.0 per cent and 66.7 per cent for uretero-ileocystoneostomy. This order was actually the same as that of the incidence of hyperchloremic acidosis.

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Tetracycline fluorescence test in urine sediment which was induced by oral administration of tetracycline (Achromycin V) was applied to patients with various urological conditions and healthy adults.
The results were as follows:
1) All 15 healthy adults gave a negative test.
2) In a study of 20 patients with urinary malignancy, 16 patients (80%) gave a positive test.
3) Of 7 patients with benign urinary lesion and 2 benign papilloma of the bladder, only 1 gave a positive test.
4) The localization and nature of fluorescent material in urine sediment were discussed.
In conclusion, this test is of value not only as a diagnostic method, but as a screening test for malignant neoplasm of urinary tract.

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