β-glucuronidase activities of serum, urine and exudate from the bladder, which were collected from the patients with genitourinary diseases, especially bladder tumors, were studied.
The results were as follows:
1) β-glucuronidase activity in urine indicated a high level in the morning and low in the afternoon.
2) There were not so remarkable changes between urine volume and β-glucuronidase activity in one day.
3) An adequate incubation time for determination of β-glucuronidase activity was 24 hours. No bacterial affects on β-glucuronidase could be found.
4) In normal, they have shown almost the same levels of β-glucuronidase activity in separate urine from the kidneys and bladder. The other hand, the patients with bladder tumors, especially cancers, their β-glucuronidase activity showed abnormal high levels in bladder urine and exudate from the bladder after urinary diversion. But after extirpation of bladder tumors, it indicated a normal β-glucuronidase activity in bladder urine.
5) A normal level of β-glucuronidase activity in urine collected from the kidneys and bladder was below 1.30μg/ml/hr.
6) A high urinary β-glucuronidase activity of bladder tumors was inhibited by treatments with SLA and other various anti-cancer drugs.
7) We found two obvious peaks, during study of β-glucuronidase isozymes pattern. We consider that there are two isozymes in β-glucuronidase.
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