Nippon Shokakibyo Gakkai Zasshi
Online ISSN : 1349-7693
Print ISSN : 0446-6586
Volume 62 , Issue 9
Showing 1-12 articles out of 12 articles from the selected issue
  • [in Japanese]
    1965 Volume 62 Issue 9 Pages 1051-1069
    Published: 1965
    Released: June 17, 2011
    JOURNALS FREE ACCESS
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  • [in Japanese]
    1965 Volume 62 Issue 9 Pages 1071-1087_3
    Published: 1965
    Released: June 17, 2011
    JOURNALS FREE ACCESS
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  • [in Japanese]
    1965 Volume 62 Issue 9 Pages 1089-1103_3
    Published: 1965
    Released: June 17, 2011
    JOURNALS FREE ACCESS
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  • Hepatic cholesterol biosynthesis and fecal excretion of endproducts of cholesterol metabolism
    Haruo Nakamura
    1965 Volume 62 Issue 9 Pages 1105-1114
    Published: 1965
    Released: June 17, 2011
    JOURNALS FREE ACCESS
    The mechanism of the hypocholesterolemic action of ursodesoxycholic acid (α-3, β-7-dihydroxycholanic acid) that were used as choleretic is not yet understood clearly. In an atempt to throw some light on the mechanism of this hypocholesterolemic action, studies were made on the incorporation of acetate-1-14C or mevalonic acid-2-14C and the fecal excretion of choleserol endproducts originating from mevalonic acid-2-14C. Male mice were used throughout the study and were placed on a cholesterol-free diet for 2 week observation period.
    1. Biosynthesis:
    Ten mice remained on the cholesterol-free diet and another ten mice were fed by supplementing the cholesterol-free diet with 0.5% ursodesoxycholic acid for 10 day. At the end of experimental period, one hour before killing the mice, the animals were injected with 5μc of acetate-1-14C or 0.12μc of mevalonic acid-2-14C per 20gm of body weight. The livers were excised and the incorporation into total lipid, cholesterol, fatty acid, protein and glycogen were measured. Ursodesoxycholic acid inhibited the incorporation of acetate-1-14C into total lipid, cholesterol and fatty acids and stimulated the incoration into protein and glycogen. The incoporation of mevalonic acid-2-14C into total lipid and cholesterol were inhibited, while the incorporation into protein remained unaltered. Ursodesoxycholic acid resulted in depression of the serum and hepatic chole. sterol level and elevation of hepatic glycogen and liver weight.
    The present data indicate that the locus of the effect of ursodesoxycholic acid on a rate-controlling step in cholesterol biosynthesis appears to lie some-where between me valonic acid and cholesterol and between acetate and mevalonic acid.
    2. Fecal excretion:
    One hour after the single injection of mevalonic acid-2-14C, animals were fed a cholesterol free diet containing 0.5% ursodesoxycholic acid for 7 days. The feces collected duaring 7 days were extracted with hot ethanol and this extracts were analysed for bile acid and sterol by the technique of Mosbach et al. The total-14C activity (ethanol extract), the total sterol-14C activity and the total bile acid-14C activity were measured. Ursodesoxycholic acid greatly increased total-14C activity excretion, the stimulation occuring in the total sterol-14C activity, while excretion of the bile acid-14C activity wa deppresed.
    It is concluded that the serum cholesterol-lowering effect of ursodesoxycholic acid appers to be due mainly to the inhibition of hepatic cholesterol bisoynthesis and the increase of fecal excretion of sterols.
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  • [in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
    1965 Volume 62 Issue 9 Pages 1115-1120
    Published: 1965
    Released: June 17, 2011
    JOURNALS FREE ACCESS
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  • [in Japanese]
    1965 Volume 62 Issue 9 Pages 1121-1129
    Published: 1965
    Released: June 17, 2011
    JOURNALS FREE ACCESS
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  • [in Japanese], [in Japanese]
    1965 Volume 62 Issue 9 Pages 1129-1133
    Published: 1965
    Released: June 17, 2011
    JOURNALS FREE ACCESS
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  • [in Japanese], [in Japanese]
    1965 Volume 62 Issue 9 Pages 1135-1140
    Published: 1965
    Released: June 17, 2011
    JOURNALS FREE ACCESS
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  • 1965 Volume 62 Issue 9 Pages 1141-1152
    Published: 1965
    Released: June 17, 2011
    JOURNALS FREE ACCESS
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  • 1965 Volume 62 Issue 9 Pages 1153-1158
    Published: 1965
    Released: June 17, 2011
    JOURNALS FREE ACCESS
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  • 1965 Volume 62 Issue 9 Pages 1159-1171
    Published: 1965
    Released: June 17, 2011
    JOURNALS FREE ACCESS
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  • 1965 Volume 62 Issue 9 Pages 1173-1181
    Published: 1965
    Released: June 17, 2011
    JOURNALS FREE ACCESS
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