Potassium conservation, i.e. progressive decrease in the excretion of potassium into the urine during potassium-deficient feeding, was observed in normal and chronic CCl4 intoxicated rats, and found to be impaired in the latter. Fourty-eight male rats, weighing 170 to 270g, were devided into the placebo control and CCl4 treated group, to which 0.1ml per 100g of olive oil or 20% CCl4, respectively, was injected subcutaneously twice a week for three weeks. In this period rats were kept on standard diet (K:0.30mEq/g). Thereafter they were kept on mild (K:0.089mEq/g) or strict (K: 0.0056mEq/g) potassium-deficient diet for 3 to 10 days. Five hours' urine after previous electrolyte loading was colected before and after potassium-deficient feeding. Blood and muscle samples were taken at the end of potassium-deficient feeding. Water, K, Na, Cl and creatinine in these materials were measured. 1) In the CCl4 treated rats kept on standard diet, urinary elimination of K after electrolyte loading was larger, that of Na was smaller and urinary K/Na ratio was higher than those in the control rats. 2) In the control rats kept on potassium-deficient diet, urinary elimination of K decreased (p<0.01), that of Na temporarily decreased and then increased (p<0.05), and the K/Na ratio fell remarkably (p<0.01). 3) In the CCl4 treated rats kept on strict potassium-deficient diet, the urinary eimination of K decreased and K/Na ratio fell as well as in the control rats. In the CCl4 treated rats kept on mild potassium-deficient diet, however, potassium concervation was impaired, i.e. the urinary elimination of K on the 3rd day and K/Na ratio on the 10th day were significantly greater than those in the control rats.(p<0.05). 4) In both control and CCl4 treated rats serum K level decreased and Na level increased after 10 days of potassium-deficient feeding. However, these changes were not statistically significant. 5) Water content of muscle was unaffected by both CCl4treatment and potassium-deficient feeding. But K content of muscle in the CCl4 treated rats was smaller and Na content was larger than those in the control rats. K/Na ratio in muscle in CCl4 treated rats was lower than that in the control rats (p<0.05). 6) Serum level and urinary output of creatinine were greater in the CCl4 treated rats, but without any significant change increatinine clearance. The present results may show the usefulness of these experimental conditions in the investigation on electrolyte abnormalities in liver damage.
Effects of chlorothiazide on urine volume and urinary excretion of electrolytes were observed in 9 normal and 9 chronic CCl4-intoxicated rats, and also the effects of chlorothiazide were observed in these rats during potassium-deficient feeding. Three different doses (0, 2.5, 10mg/rat) of chlorothiazide suspended in 5ml of distilled water were administered to each rat by mouth on 3 successive days, and 5 hours' urine was collected for analysis of K, Na and Cl. The whole procedure was first carried out during feeding with standard diet (K: 0.30mEq/g), and was repeated after 4 days of feeding with strict potassium-deficient diet (K: 0.0056mEq/g). 1) The effects of chlorothiazide during feeding with standard diet were as follows: (a) In the control rats the diuretic, kaliuretic and chloriuretic effects of 2.5mg of chlorothiazide were statistically significant (p<0.05, p<0.05 and p<0.025, respectively). But the natriuretic effect was non-significant. All these effects were statistically significant when 10mg of chlorothiazide was given to these rats (p<0.005, p<0.005 and p<0.025, respectively). (b) In CCl4-intoxicated rats, the diuretic effect of chlorothiazide was diminished, the kaliuretic and chloriuretic effects were potentiated and the natriuretic effect was not significantly changed as compared with the control rats. But these changes were not statistically significant. (2) Changes in the effects of chlorothiazide induced by potassium-dificient feeding were as follows: (a) The diuretic, kaliuretic, natriuretic and chloriuretic effects did not show any significant difference between the control and CCl4-intoxicated rats. (b) The diuretic effect was potentiated in both control and CCl4-intoxicated rats, but this change was not statistically significant. (c) The kaliuretic effect was diminished in the control rats (p<0.01) but did not show any significant change in the CCl4-intoxicated rats. (d) The natriuretic effect was potentiated in both control and CCl4-intoxicated rats, but this change was statistically significant only in the former (p<0.05). (e) The chloriuretic effect was potentiated in both control and CCl4-intoxicated rats, but this change was statistically significant only in the latter (p<0.05).
In order o simplify the Indocyanine green test, dye densitometry was applied to Indocyanine green test.The equipments used in this method were made in Japan. Procedure of the test was as follows; After the equipments were warming up for 20 min. earpiece placed on conch. Indocyanine green of 0.25mg per kilogram was injected into cubital vein for 1min. The concentration curve recorded in roll chart in speed of 2.5mm per second was analyzed. The curve was divided two phases, peak phase and declining phase. It is clear that the peak phase indicated the one for mixing of the dye, while the declining phase for dye excretion. Dereasing ratio was defined as ratio of value in 1min. after finish of injection to that of 6 min. In 50 patients with or without liver disease the decreasing ratio was compared with disappearance rate, K, which was described previously. There was a higher correlation between the decreasing ratio and eisappearance rate. From these results it seems reasonable to conclude that Indocyanine green test with earpiecedensitometry is a good and simple test and generally earpiece method may be a new laboratory test in near future.