In order to study the difference in the molecular structure of nucleic acids and nucleoproteins of carcinoma tissues from those of normal tissues, infrared spectra of these substances were analysed. The nucleic acids and nucleoproteins were extracted from the carcinoma tissues and normal tissues of gastrointestinal tracts in the human body, AH 130 ascites tumors, DAB hepatomas and the liver of normal rats. The infrared spectra of these extract-substances were determined by using KBr tablets at 18°C and 40% relative humidity. It was not possible to differentiate DNA of normal tissues from that of malignant tumors by means of infrared spectra, but possible to differentiate DNA of human tissues from that of rat tissues. Comparing DNP of the normal tissues with that of malignant tumors, the absorption bands in the 1700 to 1300cm-1 region in the human body, in the 1750 to 1400cm-1 region in AH 130 ascites tumors, in the 1600 to 1400cm-1 region in DAB hepatomas were differed from each other. For RNA the intensity of the absorption bands were differed in the 1550 to 1350cm-1 region and in the 995cm-1 band in the liver of normal rats and AH 130 ascites tumors. With DAB feeding, 1525cm-1 band of RNA of DAB hepatomas has gradually increased in intensity, whereas 1485cm-1 band has gradually decreased. Comparing RNP of the normal tissues with that of malignant tumors, the absorption bands in the 1650 to 1250 cm-1 region in the human body, in intensity of 1455cm-1 band and 1385cm-1 band in AH 130 ascites tumors, in the 1600 to 1300cm-1 region in DAB hepatomas were differed from each other.
Clinical patterns and pathological futures of two fatal patients with subacute hepatitis (Tisdale, 1963) were described. Case 1. A housewife, aged 39, was first seen on March 6, 1968 with two weeks' history of relapsed jaundice. Five months previously she had abnormal liver function tests lasted two months and enlarged liver. On admission she had deep jaundice, slight enlarged liver, palpable spleen, marked elevation of serum transaminase (S-GOT>S-GPT), γ-globulinemia, and negative LE-cells. Administration of corticosteroid for fourtysix days failed to release progressive jaundice, malaise, anorexia or weakness. Necropsy was performed on May 6, 1968. Liver histology revealed extensive necrosis of liver cells in centralobular areas, heavy lymphocyte infiltration and marked reticular fibrosis in the portal zones. Case 2. A male massagist, aged 40, was admitted on May 29, 1968 with two months' history of jaundice, anorexia, and ascites. On admission he had deep jaundice, moderate pyrexia, marked ascites, liver edge 5cm below the costal margin, elevated S-GOT and marked γ-globulinemia. Jaundice, malaise and ascites persisted until death supervened from terminal hepatic coma on June 20, 1968. Liver histology revealed irregular and submassive depletion of liver cells, proliferation of bile ducts in portal zones, and reformation of lobular architecture with collagenization. These findings indicated partial transition from subacute hepatic necrosis to postnecrotic cirrhosis.
Statistical analyses have been made on the relation of patient's age with various liver function tests done within 10 days after the onset of symptoms, time required for normalization of liver function tests and death rate in 250 cases of acute infectious hepatitis, admitted to our department and hospitals affiliated to our department. The following results have been obtained: 1. Among various liver function tests at the onset, serum transaminase level and the patient's age exhibited some correlation of statistical significance. There was also a correlation between the age and the concentration in percentage of serum γ-globulin. 2. The number of days required for the normalization of liver function tests, such as serum bilirubin, GPT and BSP test, was directly proportional to the patient's age, and the correlation was highly significant in the case of BSP. 3. A significant difference in death rate was noted between the group below 60 years of age and the one over 60; it was 1.3% in the former and 20.8% in the latter. The time for normalization of liver function tests was prolonged in the group over 60 years of age in comparison with the one below 60, but the difference was insignificant. No difference was noted between males and females with respect to liver functions at the onset or the time for their normalization.