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Volume 82, Issue 1
Displaying 1-23 of 23 articles from this issue
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Osamu NISHIDA, Tadashi KANO, Hideaki TSUKADA, Norimasa YASUDA, Yuko KO ...1985 Volume 82 Issue 1 Pages 1-8
Published: 1985
Released on J-STAGE: December 26, 2007
JOURNAL FREE ACCESSThe purpose of this report is to study immunocytochemical localization of lactoferrin on the sections of resected stomach by unlabeled antibody peroxydase-anti-peroxydase (PAP) method. Antilactoferrin which was used in this study was prepared in our laboratory from rabbit immunized with lactoferrin isolated from normal human colostrum. Lactoferrin was located in the neck cells of fundic glands as well as pyrolic glands. And also the bottom of fundic glands contained lactoferrin. These lactoferrin-positive cells had an ability of producing mucin which was identified by PAS staning. They were not always detectable in all the cases examined. It was of note that the lactoferrin producing cells were close contacts with distribution of lymphoid cells in the mucosa. They were found exclusively in the neighborhood of the lymphoid infiltrations, especially lymph follicles. From these data, the functional relationship between lactoferrin and lymphocytes of gastric mucosa was strongly suggested.View full abstractDownload PDF (3400K) -
Junichi KUMAGAI, Eizo KANEKO1985 Volume 82 Issue 1 Pages 9-17
Published: 1985
Released on J-STAGE: December 26, 2007
JOURNAL FREE ACCESSThe effect of gastric ulcer produced by topical procedure on gastric secretion was investigated in conscious fistula dogs. Gastric ulcer was produced by submucosal injection of acetic acid or electric cauteriztion through gastric fistula. Gastric secretion was stimulated by tetragastrin i.v. infusion. The acid output and volume of gastric juice began to decrease at 10 minutes and 20 minutes respectively after the production of ulcer, and the decrease of the output continued to the end of the experiments, one hour after the procedure. They recovered partialy by propranolol and almost completely by phentolamine administration. Gastric secretion showed no significant difference among the one before and 1, 4, 7 and 14 days after ulcer production.View full abstractDownload PDF (1243K) -
Yutaka TAKAHASHI1985 Volume 82 Issue 1 Pages 18-27
Published: 1985
Released on J-STAGE: December 26, 2007
JOURNAL FREE ACCESSThree cases of human alpha-fetoprotein (AFP) producing stomach cancer could be serially transplanted in BALB/c nu/nu nude mice. In this report, we studied on the biological characteristics of these tumors.
It was characterized that three transplanted tumors retained not only the morphology but also the function of the original tumors. Serum AFP level in nude mice ranged from 10, 690ng/ml to 38, 540ng/ml, and increased progressively. A positive correlation was observed between serum AFP level and tumor weight. It was of further interest that all of three tumors produced both AFP and normal serum proteins, albumin, α1-anti-trypsin and transferrin.
As a series of therapeutic experiment applied to this biological properties, the "missile therapy" has been administered to the transplanted nude mice bearing AFP producing stomach cancer, using a conjugate of mitomycin C and antibody to human AFP. As a result, significant inhibition was obtained in tumor growth and its effect is equivalent to that of ten times as compared with original ordinary concentration dosis of MMC.View full abstractDownload PDF (2530K) -
Hajime KUWATA, Kazuhiko ISHIHARA, Masao KAKEI, Susumu OHARA, Haruya OK ...1985 Volume 82 Issue 1 Pages 28-33
Published: 1985
Released on J-STAGE: December 26, 2007
JOURNAL FREE ACCESSQuantitative changes of gastric mucosal glycoproteins as well as macroscopical mucosal lesion induced by ethanol in rat have been studied. The mucus glycoprotein in gastric mucosa was isolated from the corpus region of the stomach excised at three and five hours after 30-100% ethanol administration.
1) Both the decrease in gastric mucus glycoproteins and the increase in macroscopical mucosal damage were related to ethanol concentration.
2) When 30-40% ethanol was administered in rats orally, the recovery of gastric mucus glycoprotein contents and from macroscopical damage was observed at five hours after dosing. However, the recovery was not observed over 50% ethanol administration.
The reduction on gastric mucus glycoproteins did not exceed about 50% of the control value, even when 70-100% ethanol was administered.View full abstractDownload PDF (713K) -
Effect of Several Anti-Ulcer Drugs on Aspirin-Induced Mucosal DamageAkira TORII, Kzutada EGAWA, Masahiro ARIIZUMI, Hitoshi IWASAKI, Hirosh ...1985 Volume 82 Issue 1 Pages 34-42
Published: 1985
Released on J-STAGE: December 26, 2007
JOURNAL FREE ACCESSThe changes of covering and epithelial mucus at the different segments of stomach with the aspirin induced mucosal damage were studied by histochemical method.
The oral administration of aspirin to rats decreased gastric covering and epithelial mucus and induced erosion with necrosis. On the contrast, in prostaglandin E2, pirenzepine, and cimetidine treated rats before the aspirin administration, the gastric mucosal damage and the decrease of mucus were suppressed. In addition, prostaglandin E2 and pirenzepine increased covering mucus comparing with the healthy control group.
Gastric mucus, especially covering mucus, is very important as a defensive factor to the prevention of aspirin induced mucosal damage.View full abstractDownload PDF (5183K) -
III. Changes of Microcirculation of Rat Gastrointestinal Tract in Experimental Disseminated Intravascular CoagulationOsamu SETO, Toshikazu YOSHIKAWA, Yasumasa FURUKAWA, Masashi MURAKAMI, ...1985 Volume 82 Issue 1 Pages 43-50
Published: 1985
Released on J-STAGE: December 26, 2007
JOURNAL FREE ACCESSThe experimental model of disseminated intravascular coagulation (DIC) in rats induced by 4-hr sustained infusion of 100mg/kg endotoxin was used to study the relationship between the blood flow of the digestive tract in DIC state and the mucosal injury. Blood pressure had decreased following the administration of endotoxin. At 3hrs after the initiation of endotoxin infusion, FDP and the number of glomeruli with fibrin thrombi had increased, PT and PTT were prolonged, and platelet counts and fibrinogen level were depressed. At 4hrs coagulation stage was further advanced, and glomerular fibrin deposits could be demonstrated in all rats and hemorrhagic esions were recognized diffusely in the small intestine.
The local blood flow of the rats measured by hydrogen clearance method showed a statistically significant decrease at the antrum of the stomach and duodenum by 2 to 4hrs after the infusion of endotoxin, and jejunum and ileum by 4hrs. It was strongly suggested that impaired microcirculation of the small intestine either by a decreased blood pressure or by hypercoagulable state in experimental DIC caused hemorrhagic lesions at the small intestine.View full abstractDownload PDF (1216K) -
Masanori OHTA, Shosei ISHIGURO, Satoru IWANE, Shigeyuki NAKAJI, Masaak ...1985 Volume 82 Issue 1 Pages 51-57
Published: 1985
Released on J-STAGE: December 26, 2007
JOURNAL FREE ACCESSEffects of dietary fibers against the development of colonic diseases such as colonic polyps and colonic diverticula were studied. Fifty cases of colonic polyps (Group 1) and 33 cases of colonic diverticula (Group 2) were subjected to the study and compared with normal controls (Group 3) who had received the National Nutrition Survey. Meals were weighed for three consecutive days according to the National Nutrition Survey, and weight of dietary fibers was estimated by dietary fiber analysis using Southgate's method.
Daily intake of dietary fibers was 18.2±5.44g, 17.4±5.07g, 21.1±6.57g, in Group 1, 2 and 3 respectively. The values of Group 1 and 2 were significantly lower than that of Group 3, and there was no difference between Group 1 and 2. As to conponents of dietary fibers, there was a significant difference in the intake of hemicellulose and cellulose and no difference in the intake of lignin between patient groups and controls.
In conclusion, it was thought that increase of dietary fiber intake might inhibit the development of colonic polyps and colonic diverticula.View full abstractDownload PDF (676K) -
Senichiro AGAWA, Tetsuichiro MUTO, Toshio SAWADA, Yukio SAITO, Miki AD ...1985 Volume 82 Issue 1 Pages 58-64
Published: 1985
Released on J-STAGE: December 26, 2007
JOURNAL FREE ACCESSMucin characteristics of serial biopsies in patients with ulcerative colitis was studied by PAT/KOH/PAS staining method in order to demonstrate mucin characteristics in active and inactive.
A total of 134 biopsy specimens from 74 patients were obtained via proctoscope or colonoscope for this study.
From our observations it became clear that blue stained sialomucin was predominant in active state whereas red stained sialomucin was mainly seen in remission, however, in some patients colonic mucin showed the same characteristics in both active and inactive state.
Staining characteristics of the active and proximal normal mucosa were equivalent in subjects examined. Those having red stained sialomucin in both active and inactive sites tend to become remitting state.
Those having red stained sialomucin in remission tend to have prolonged remitting state, whereas those having red sialomucin in active state tend to have rather short period of active disease.
All dysplastic tubules in ulcerative colitis were found to have blue stained sialomucin, and it was interesting to note that in some areas with normal appearence tubules having blue stained sialomucin were present in a mosaic pattern.View full abstractDownload PDF (2507K) -
Akira SUGITA, Tsuneo FUKUSHIMA, Naoki ISHIGURO, Masaru KAWAMOTO, Akira ...1985 Volume 82 Issue 1 Pages 65-71
Published: 1985
Released on J-STAGE: December 26, 2007
JOURNAL FREE ACCESSConcentration of serum long chain fatty acids was analysed in 13 patients with ulcerative colitis and 12 patients with Crohn's disease, using gas-chromatography. In the patients with ulcerative colitis, every fraction of fatty acid and total fatty acid was not different from that of normal controls. In the patients with Crohn's disease, concentration of palmitic, linoleic, linolenic, arachidonic acids and total fatty acid were decreased significantly. Concentration of serum linoleic acid was inversely correlated to the value of Crohn's Disease Activity Index with statistical significance (p<0.01). Decrease of serum long chain fatty acids was considered due to derease of food intake containing these fatty acids, disturbance of bile acid metabolism secondary to resection or lesion of the terminal ileum. Concentration of long chain fatty acids, especially that of essential fatty acids was found to be one of the important indices evaluating clinical and nutritional status of Crohn's disease.View full abstractDownload PDF (833K) -
Toshifumi OHKUSA, Hideaki FUJIMOTO, Fukuko SHIKATO, Masayo NISHIURA, R ...1985 Volume 82 Issue 1 Pages 72-77
Published: 1985
Released on J-STAGE: December 26, 2007
JOURNAL FREE ACCESSWe have attempted to produce experimental ulcerative colitis in Syrian hamsters by inoculating organisms of Bacteroides vulgatus into the cecum. We used an isolate of B. vulgatus from the fecal microflora of human ulcerative colitis, which showed cytopathic effects to cultured cells and resistance to clindamycin and the aminoglycosides. Prior to inoculation with the organisms, hamsters were treated with clinidamycin and tobramycin to reduce the intestinal microflora.
Four of the 12 hamsters inoculated with the organisms developed erosion and ulceration in cecal mucosa as observed histologically. One of them showed crypt abscess in cecal mucosa and 6 of 12 hamsters developed mucosal hemorrhage and congestion. All of 12 hamsters showed histological evidence of mild to severe inflammation in cecal mucosa.
Control group of 12 hamsters did not develop erosion and ulceration in cecal mucosa as observed histologically.
The results that an isolate from the fecal microflora of human ulcerative colitis produced changes similar to human ulcerative colitis in Syrian hamsters suggest that B. vulgatus play an improtant role in producing erosions and ulcers of the colon.View full abstractDownload PDF (1098K) -
Taido ARAI, Jun-ichi MATSUMOTO, Hiroshi ODAJIMA, Hiroshi SHIMOJO, Hiro ...1985 Volume 82 Issue 1 Pages 78-85
Published: 1985
Released on J-STAGE: December 26, 2007
JOURNAL FREE ACCESSWe experienced thirty nine cases of antibiotic-associated acute hemorrhagic colitis after administration of antibiotics for past five years. The aim of this report is to evaluate in these cases from the standpoint of microbiology as well as clinical and endoscopical findings. This disease developed on several days after oral administration of synthetic penicillin with broad spectrum in most of all patients. These patients showed marked improvement on symptomatic therapy and discontinuance of antibiotics within several days.
The characteristic features of endoscopic examination were redness, edema, erosion and bleeding int he deep interior colon rather than the sigmoid colon.
On the microbiological examination, Klebsiella oxytoca was recovered from 31 (79.5%) of the 39 specimens. Anaerobic stool culture was performed on 27 of the 39 specimens. Clostridium difficile was recovered from 10 (37.0%) of the 27 specimens, and Clostridium sordellii was recovered from 2 (7.4%). These all clostridia were toxin-producing strains. Assay for Colstridium difficile toxin (D-1) was performed on five stools by Latex agglutination test. It was positive in 4 of the 5 specimens.
These findings suggest a possivility that toxin-producing clostridia leads to a part of antibiotic-associated acute hemorrhagic colitis.View full abstractDownload PDF (987K) -
Toshinobu TAKAHASHI1985 Volume 82 Issue 1 Pages 86-93
Published: 1985
Released on J-STAGE: December 26, 2007
JOURNAL FREE ACCESSThe purpose of the present investigation is to describe the application of the quantitative analysis of lactate dehydrogenase and glutamic-oxaloacetic transaminase in the endoscopic biopsy specimen for the determination of the tendency of malignancy of the colon polyp.
Significant differences were observed when the ratio of the LDH to GOT enzyme present in the endoscopic biopsy is taken into consideration as index.
In the normal mucosa the LCH/GOT ratio was of 1.37±0.2, in the colon polyp was of 2.58±0.8, and of 3.50±1.3 in the colon cancerous tissue.
At that time a strong correlation is seen between the LDH/GOT ratio and the grade of atypia detected by the histology. The higher the grade of atypia, an increase of the LDH/GOT ratio is noticed.
Therefore, in the cases of polyp with focal cancer, the LDH/GOT ratio is seen in close proximity of the LDH/GOT ratio found in the colon cancerous tissue.
In conclusion LDH/GOT ratio in the endoscopic biospy specimen is found to be a simple and useful diagnostic method in the existence of the tendency of malignancy of the colon polyp.View full abstractDownload PDF (1262K) -
Takayuki ARAI, Yasuhiro MIZOGUCHI, Hiroko KATOH, Hiroko TSUTSUI, Keiji ...1985 Volume 82 Issue 1 Pages 94-99
Published: 1985
Released on J-STAGE: December 26, 2007
JOURNAL FREE ACCESSKupffer cells isolated from rat liver were treated with sex hormones and their culture fluids were added to the isolated rat liver cells. When Kupffer cells were treated with estrogens, their culture supernatants caused the decrease in protein synthesis of the isolated liver cells, suggesting that a hepatocytotoxic substance (or substances) was produced from the estrogen-treated Kupffer cells. On the other hand, this hepatocytotoxicity resulted from the estrogen-treated Kupffer cell culture fluid was not demonstrated when Kupffer cells were treated with estrogen in the presence of testosterone.
These results suggest that estrogen may activate Kupffer cells and produce same kind of hepatocytotoxic factor, and testosterone may compete with estrogen for Kupffer cell activation.View full abstractDownload PDF (756K) -
On the Metabolism of PolyamineShoji KUBO, Isao MATSUI, Shuzo OTANI, Seiji MORISAWA, Hiroaki KINOSHIT ...1985 Volume 82 Issue 1 Pages 100-107
Published: 1985
Released on J-STAGE: December 26, 2007
JOURNAL FREE ACCESSThe effect of splenectomy on hepatic polyamine content and DNA synthesis was studied in rat liver regeneration after partial hepatectomy. The activity of ornithine decarboxylase (ODC), a rate-limiting enzyme in polyamine biosynthesis, markedly increased in the early stages of liver regeneration, and the increase in this enzyme activity was significantly enhanced in splenctomized rats. The effect of splenectomy was specific for the induction of ODC, since tyrosine aminotransferase activity was not affected. Splenectomy also enhanced increases in hepatic polyamines and DNA synthesis in the regenerating liver. These results suggest that splenectomy affects liver regeneration after partial hepatectomy by enhancing the induction of ODC activity, which is an important early biochemical event in liver regeneration.View full abstractDownload PDF (963K) -
Yoshitomo ISHII1985 Volume 82 Issue 1 Pages 108-117
Published: 1985
Released on J-STAGE: December 26, 2007
JOURNAL FREE ACCESSCytotoxicity of chenodeoxycholic acid (CDCA) on hepatocytes was studied using primary cultured rat hepatocytes and the cytoprotective effect of prostaglandin E1(PGE1) was also investigated.
Marked elevation of LDH activity was noted in the culture medium of rat hepatocytes containing 0.5mM/L of CDCA even after 30 minutes incubation. Electron microscopy showed remarkable changes of the plasma membranes of cultured hepatocytes with minimal alteration of cytoplasmic organelles in the medium containing 0.5mM/L of CDCA. Addition of PGE1 in the medium minimized the release of LDH from hepatocytes cultured in the medium containing CDCA, and ultrastructural changes of the plasma membrane of cultured hepatocytes were much less conspicuous. These data indicate that PGE1 may have a beneficial effect on the changes of cultured hepatocytes induced by CDCA.View full abstractDownload PDF (3485K) -
Yasuna SUZUKI, Kiyoshi OKAMURA, Hisato HARA, Toshifumi ASHIDA, Makoto ...1985 Volume 82 Issue 1 Pages 118-122
Published: 1985
Released on J-STAGE: December 26, 2007
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Masahiro SUENAGA, Tadayuki KANOH, Kohichiroh TAGAMI, Toshiaki NONAMI, ...1985 Volume 82 Issue 1 Pages 123-127
Published: 1985
Released on J-STAGE: December 26, 2007
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T. CHIKAMA, S. HORITA, H. TSUKAGOSHI, T. YAOSAKA, T. SUGA, Y. MURASHIM ...1985 Volume 82 Issue 1 Pages 128-133
Published: 1985
Released on J-STAGE: December 26, 2007
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Yasuyuki ARAKAWA, [in Japanese], [in Japanese], [in Japanese], [in Jap ...1985 Volume 82 Issue 1 Pages 134
Published: 1985
Released on J-STAGE: December 26, 2007
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With special reference to plasma pivka-II as a marker of hepatocellular carcinomaShigetoshi FUJIYAMA, [in Japanese], [in Japanese], [in Japanese], [in ...1985 Volume 82 Issue 1 Pages 135
Published: 1985
Released on J-STAGE: December 26, 2007
JOURNAL FREE ACCESSDownload PDF (151K) -
Norio UENO, [in Japanese], [in Japanese]1985 Volume 82 Issue 1 Pages 136
Published: 1985
Released on J-STAGE: December 26, 2007
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Tomohiko YOSHIDA, [in Japanese], [in Japanese], [in Japanese], [in Jap ...1985 Volume 82 Issue 1 Pages 137
Published: 1985
Released on J-STAGE: December 26, 2007
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Hiroaki MIYAOKA, [in Japanese], [in Japanese], [in Japanese], [in Japa ...1985 Volume 82 Issue 1 Pages 138
Published: 1985
Released on J-STAGE: December 26, 2007
JOURNAL FREE ACCESSDownload PDF (139K)
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