Nippon Shokakibyo Gakkai Zasshi
Online ISSN : 1349-7693
Print ISSN : 0446-6586
Volume 83 , Issue 11
Showing 1-25 articles out of 25 articles from the selected issue
  • Sunao KAWANO, Masuki FUKUDA, Shingo TSUJI, Kouichi NAGANO, Tatsuo OGIH ...
    1986 Volume 83 Issue 11 Pages 2297-2301
    Published: 1986
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The esophageal mucosal blood volume and the estimated oxygen saturation of hemoglobin in the capillaries were investigated in patients with liver cirrhosis using reflectance spectrohotometry.
    In normal controls, the index of esophageal mucosal blood volume was 0.58±0.11 and the estimated oxygen saturation of Hb was 62±7%. These parameters did not changed by aging.
    In patients with liver cirrhosis, the index of esophageal mucosal blood volume increased and the estimated oxygen saturation of Hb decreased significantly, when compared with those of controls. The changes in these parameters were greater in cirrhotic patients with esophageal varices than in cirrhotic patients without esophageal varices.
    The results suggested that the esophageal mucosal microcirculation was congested in patients with liver cirrhosis. This may weaken defensive factors, leading to the rupture of the esophageal varix.
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  • Takashi KAWAI, Takeshi AZUMA, Hideto INOKUCHI, Zenji SASAKI, Keiichi K ...
    1986 Volume 83 Issue 11 Pages 2302-2309
    Published: 1986
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The effects of omeprazole, a proton pump inhibitor, on the secretion of acid and pepsin in Wistar rats of 4-W age were studied by a subcutaneous injection, and that on the neonatal development of gastric mucosa was also evaluated by long term administration of omeprazole (s.c) in new born rats. Single subcutaneous administration of omeprazole showed dose-dependent inhibition on acid secretion in 4w rats, particularly, 50mg/kg omeprazole produced total inhibition for 72 hours. Omeprazole also inhibited pepsin secretion. Four weeks administration of 50mg/kg omeprazole to new born rats caused 1) marked vacuolar degeneration of fundic mucosa with eosinophilic change of chief cells, 2) marked increase of serum gastrin levels, and 3) significant increase of pepinogen proteolytic activity of fundic mucosa. These changes were confirmed to be transient and reversible after cessation of the administration.
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  • Hajime KUWATA, Kazuhiko ISHIHARA, Susumu OHARA, Masao KAKEI, Tsutomu K ...
    1986 Volume 83 Issue 11 Pages 2310-2317
    Published: 1986
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The effects of two synthetic prostaglandin E derivatives, 17S, 20-dimethyl-6-oxo PGE1 methyl ester (PGE1) and 15 (R)-15-methyl PGE2 (PGE2) on the gastric mucus glycoproteins were estimated by the following methods using rat gastric mucosa.
    1) The mucus glycoproteins in gastric mucosa were isolated from corpus and antral region after single oral administration of PGE1 or PGE2 (100μg/kg). Two PG derivatives caused no significant changes in the content and carbohydrate composition of mucus glycoprotein compared with those of control in both regions.
    2) Synthesis of mucus glycoproteins, as assessed by incorporation of (3H)-glucosamine in the organ culture system, was accelerated to 115-130% of control by oral pretreatment with two PG derivatives.
    3) Three distinct histochemical methods specific for mucin staining showed marked increase and/or emergence in staining of mucin on the mucus layer with PG treatment.
    In summary, these results indicate that PG derivatives enhance the metabolic turnover of gastric mucus glycoproteins.
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  • Kazutaka YAMADA, Sonshin TAKAO, Shigeho MAENOHARA, Tetushi SAIHARA, At ...
    1986 Volume 83 Issue 11 Pages 2318-2324
    Published: 1986
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Tumor lines xenografts in nude mice used in this study include COK-1 and COK-7. COK-1(PT, LN and RE) has been established from the primary (PT), lymph node metastatic (LN) and local recurrent (RE) tumors of human colon cancer, and COK-7 (PT and LiM) has been established from the primary (PT) and liver metastatic (LiM) tumors of human rectal cancer. These tumor lines were used for the study of chemotherapeutic responses to such anti-cancer drugs as 5-FU, MMC, CPM and CDDP. Chemotherapeutic responses to these drugs in each tumor line were as follows.; COK-1 (PT) responded to only MMC, while COK-1 (RE) responded to both MMC and CDDP. However, COK-1 (LN) did not respond to any drug studied. In case of COK-7 (PT) it did not respond to any drug as well, though COK-7 (LiM) showed a response to MMC.
    These results indicate that each tumor line of COK-1 and COK-7 has chemosensitive differences in primary, metastatic and recurrent tumor lines.
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  • Yoichi HAGA
    1986 Volume 83 Issue 11 Pages 2325-2333
    Published: 1986
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Recently Chiodini, et al. described isolation of Mycobacterium species from several patients with Crohn's disease (CD). These data prompted us to investigate the relationship between M. Paratuberculosis and CD. Using ELISA, no difference was observed between CD and ulcerative colitis (UC) in antibody titers against M. paratuberculosis in any classes of immunoglobulin, although the titers of CD were slightly higher than those of controls in IgG and IgA classes (P<0.05). Using Herrold's egg yolk medium (HEYM) with mycobactin, we attempted to isolate organisms from tissue specimens and stools. After three weeks of culture, whity spots were seen on the HEYM in which the sample of the biopsy specimen from a patient with CD was added. These isolates were coccoid and positively stained with Ziehl-Neelsen method showing dissimilarity to the Mycobacteria isolated by Chiodini, although subculture was not successful. Using PAP method with antibody against M. paratauberculosis, we attempted to stain intestinal specimens from patients with CD, resulting in no positive stain in any specimen. In conclusion, we could not get any evidence to support that M. paratuberculosis plays an etiological role in CD.
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  • Regeneration of Intestinal Epithelium from the Brunner's Glands
    Ken IGARASHI, Tsugio HIGUCHI, Hiroshi SUTO
    1986 Volume 83 Issue 11 Pages 2334-2340
    Published: 1986
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    Generally, it is considered that the regeneration of duodenal ulcers is performed by intestinal epithelium in the margin of the ulcers, while in this study the role of the Brunner's glands in the healing process of duodenal ulcers was investigated.
    The Brunner's glands were exposed with operative skinning of rats' duodenal epithelium. This area was surrounded by cauterised ulcer, so that the regenerated intestinal epithelium did not reached into the Brunner's glands area. The histological changes of the isolated Brunner's glands were investigated at 2 to 28 days after operation. The Brunner's glandular cells of the outer layer flattened, covering the surface of the ulcer, then changed gradually to the high columner cells, and finally differentiated into the intestinal epithelium and goblet cells. The Brunner's glands of the inner layer also differentiated into the intestinal epithelium.
    From these results, it was directly evidenced that not only the intestinal epithelium in the margin of the ulcer, but also the Brunner's glands formed the intestinal epithelium in the healing process of duodenal ulcer.
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  • Hideto TSUKAMOTO
    1986 Volume 83 Issue 11 Pages 2341-2350
    Published: 1986
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    One hundred and ninety-six cases with fungus infections of the digestive tract were encountered in pathological autopsy cases in the Kitasato University Hospital during past 12 years (1971-1983), and were submitted for this study. Its incidence to total autopsy cases was 5.6 percent, and the most of them were determined as terminal infections in cases with malignant tumors and leukemia. The majority of the cases were candidiasis, and a small number of aspergillosis and cryptococcosis were also recognized. Lower part of the esophagus and middle part of the stomach were the most frequently affected sites, and intestinal lesions were low in incidence. Fungal lesions were macroscopically classified into three groups, i.e., erosive, pseudomembranous, and ulcer-associated types. Pseudomembranous type was the predominant lesion of the esophagus, and the ulcer-associated type was most frequent in the stomach. Nausea, vomiting, diarrhea, and gastrointestinal bleeding were common symptons, and their clinical duration was within two weeks in a vast majority of cases with severe fungus infections. It should be mentioned that the lesions of the digestive tract were recognized as the primary focus in most cases with systemic candidiasis.
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  • Kenji TAKABATAKE, Noriyuki KITAMI
    1986 Volume 83 Issue 11 Pages 2351-2358
    Published: 1986
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    In order to clarify the mechanism of lymphocyte cytotoxicity for liver cells, the BR (Binding Ratio) of peripheral lymphocytes to isolated rat liver cells was determined in patients with acute non-type-A hepatitis, acute type-A hepatitis, chronic active hepatitis and liver cirrhosis. A significant correlation between serum GOT levels and BR values was found in acute non-type-A hepatitis and chronic active hepatitis, and BR showed significantly low values in acute phase of acute type-A hepatitis. When autologous serum or homologous AB type serum was added in this experimental system, BR of acute non-type A hepatitis and chronic active hepatitis correlated with GOT levels, and BR increased in recovery phase of acute non-type-A hepatitis when autologous serum was added. In acute phase of acute type-A hepatitis BR did not change when serum was added, but in recovery phase BR changed as similarly as that of acute non-type-A hepatitis.
    From these results it is suggested that the liver cell cytotoxicity in acute type-A hepatitis is different from that of acute non-type-A hepatitis and of chronic active hepatitis.
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  • Yutaka INAGAKI, Nobuyoshi TANAKA, Kazuo NOTSUMATA, Yuichi NAKAMURA, Ya ...
    1986 Volume 83 Issue 11 Pages 2359-2364
    Published: 1986
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    To investigate insulin-like growth factor-I (IGF-I) in patients with various chronic liver diseases, we measured the serum levels of IGF-I using radioimmunoassay. The mean values of serum IGF-I in normal controls and patients with asymptomatic HBsAg carrier, chronic inactive hepatitis, chronic active hepatitis, liver cirrhosis and hepatocellular carcinoma were 257±67, 255±103, 251±84, 161±67, 52±47 and 52±46fmol/ml, respectively. The levels of IGF-I were correlated with those of serum albumin and hepaplastin test, and inversely correlated with the values of ICG (R15). In cirrhotic patients, the serum levels of IGF-I in the hepatic veins were decreased, but those of growth hormone in the peripheral veins were increased.
    These findings indicated that decreased production of IGF-I in the liver reflected the reduction of hepatic reserve in patients with advanced chronic liver diseases.
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  • A Comparative Study with Fatty Liver of Other Origin
    Kazuo TARAO, Akira SAKURAI, Kazuhiro HAYASHI, Toshio IKEDA, Eison CHIN ...
    1986 Volume 83 Issue 11 Pages 2365-2374
    Published: 1986
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    The incidence of palmar erythema was studied in 43 patients who had histologically proven fatty liver with negative HBs-Ag and without history of blood transfusion, acute hepatitis nor jaundice. Incidence of palmar erythema in 17 patients with alcoholic fatty liver was so high as 82% (14 out of 17), and that in alcoholic fatty liver accompanied by obesity or diabetes mellitus was 71% (5 out of 7). In contrast, the incidence in 19 fatty liver patients without ethanol intake was as low as 16%. Significant difference (P<0.005) was found between alcoholic fatty liver group and the fatty liver group without ethanol intake. Also significant difference (P<0.025) was found between alcoholic fatty liver group accompanied by obesity or diabetes mellitus and the group without ethanol intake. The estrogen levels in serum and urines estimated in five patients with alcoholic fatty liver and palmar erythema proved to be in normal range. Malnutrition did not exist in those patients with alcoholic fatty liver.
    Hence, it is concluded that high incidence of palmar erythema in alcoholic fatty liver may be associated with direct effect of ethanol on the vasculature.
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  • Shujiro SUGITA, Kunihiko OHNISHI, Akira HAYASAKA, Takafumi TSUNODA, Hi ...
    1986 Volume 83 Issue 11 Pages 2375-2382
    Published: 1986
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    One hundred and forty patients with hepatocellular carcinoma were treated by transcatheter arterial administration of anticancer drugs. Out of 140 patients, 52 were treated by Mitomycin C (MMC), 32 were treated by Mitomycin C microcapsules (MMCmc), 34 were treated by MMCmc+transcatheter arterial embolization (TAE) and 22 were treated by MMCmc+Adriamycin (ADR)+TAE.
    The one year survival rates for the groups treated with MMC, MMCmc, MMCmc+TAE and MMCmc+ADR+TAE were 8.8%, 16.9%, 51.8% and 46.8%, respectively. The percent regression of the tumor size was evaluated on angiograms, computed tomograms and ultrasonograms at 4 or 5 weeks after the first treatment. Frequency of tumor regression of more than 50% after MMC, MMCmc, MMCmc+TAE and MMCmc+ADR+TAE treated groups was 12.5%, 27.6%, 59.3% and 52.6%, respectively, 25% to 50% regression occurred in 12.5%, 20.7%, 33.3% and 21.2%, less than 25% regression in 6.3%, 44.8%, 3.7% and 10.5%, while there was no response in size in 68.8%, 6.9%, 3.7% and 15.7%, respectively.
    Serum AFP levels greater than 300ng ml before treatments, fell definitely in the decreasing order of MMC, MMCmc+TAE and MMCmc+ADR+TAE groups in incidence.
    The side effects such as myelosuppression, hepatotoxicity and nephrotoxicity were much stronger in the TAE treated cases than in other cases.
    The pathological findings were investigated in 15 operated patients including 3 MMC treated cases, 3 MMCmc treated cases, 6 MMCmc+TAE treated cases and 3 MMCmc+ADR+TAE cases. Total necrosis of tumors occurred in all TAE treated cases and in 2 cases of 3 MMCmc treated cases, and partial necrosis in 1 out of 3 MMC treated cases, but some viable cancer cells were seen within the capsule.
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  • Establishment of a Radioimmunoassay Specific for CCK33
    Ryo HOSOTANI, Kazutomo INOUE, Junichi TAKAHASHI, Masafumi KOGIRE, Yu-S ...
    1986 Volume 83 Issue 11 Pages 2383-2392
    Published: 1986
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    We have established a radioimmunoassay system specific for CCK33. With the use of this assay system, we have examined the CCK release in man and dogs, and also investigated the relationship between the CCK release and exocrine pancreatic response or gallbladder movement. The validation study in vitro showed that this assay system was sensitive, specific for CCK33 with no crossreaction on gastrin, and free from the influence of interfering substances in plasma. Significant rises in circulating CCK levels were observed in response to intraduodenal infusions of both fatty acid in eight dogs (1.7± 0.3nmol-150min/L) and egg yolk in eight subjects (from a basal of 14±2 to a peak of 42±6pmol/L). The CCK release was highly correlated with pancreatic secretion in dogs and gallbladder contraction in man. These findings suggest that CCK33 may be largely responsible for the physiological mechanisms of exocrine pancreatic secretion and gallbladder contraction.
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  • Nobuyuki TASHIMA
    1986 Volume 83 Issue 11 Pages 2393-2401
    Published: 1986
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    In order to clarify the cytoplasmic character in carcinoma of gallbladder, dysplastic epithelium, and mucosa of chronic cholecystitis, totally 60 cases of surgically resected gallbladders (29; carcinomas of gallbladder, 31; chronic cholecystitis) were examined with lectins (Ulex europaeus agglutinin-1 (UEA-1) and Peanut lection (PNA)) and Carcinoembryonic Antigen (CEA).
    In chronic cholecystitis both covering epithelium and Rokitansky-Aschoff sinuses were stained well with UEA-1, but, neither covering epithelium nor Rokitansky-Aschoff sinuses were stained with PNA or with CEA. Pseudo-pyloric glands were stained with UEA-1 and with PNA, but they were not stained with CEA. Hyperplastic epithelium in chronic cholecystitis was stained with UEA-1 and with PNA in half of cases examined. However, the hyperplastic epithelium was not stained with CEA.
    Respectively, in most cases of carcinoma of the gallbladder, carcinoma cells were stained with UEA-1, as well as covering epithelium in chronic cholecystitis. Carcinoma cells were also stained with PNA and CEA, though covering epithelium in chronic cholecystitis was stained neither with PNA nor with CEA. In carcinoma, the binding sites of UEA-1 or PNA were not always consistent with positive sites for CEA. In contrast to well staining in the covering epithelium, dysplastic epithelium next to carcinoma was stained negative or little on cell apex with UEA-1, showing the loss of fucosyl glycoproteins, especially of blood group H (O) substance. The dysplastic epithelium was stained weakly positive on cell apex with PNA and with CEA, and weakly positive in cytoplasm with PNA.
    These findings suggest that change of UEA-1 binding glycoproteins and PNA binding glycoproteins is induced in the process of canceration. And in comparison with the findings of CEA which appeared especially on carcinoma, the localization of the glycoproteins differs even among morphologically similar cells.
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  • Using Biopsied Specimen Taken by Percutaneous Transhepatic Cholangioscopy
    Hideo KISHIMOTO, Yuzi FUTAMURA
    1986 Volume 83 Issue 11 Pages 2402-2410
    Published: 1986
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    CEA and CA19-9 were localized immunohistochemically in biliary tract tissues taken by percutaneous transhepatic cholangioscopic biopsy with the indirect enzyme-labeled antibody technique by Nakane.
    The relations of the localization pattern of CEA and CA19-9 to the atypism of the tissues, histological patterns of the carcinomas, and serum level of both CEA and CA19-9 were as follows:
    1) The polar distribution of CEA and CA19-9 on the cancer cells was lost and the stromal staining of these antigens appeared in the parallel to the cellular atypism.
    2) Degree of cancer differentiation had a correlation with the localization pattern of CEA and CA19-9 in the cancer cells.
    3) One of the important factors for the elevation of these serum levels might be caused by diffusion of these antigens into the interstition, and subsequently into blood vessels and lymphatics.
    These results suggest that the immunohistochemical analysis of CEA and CA19-9 localization in specimens taken by percutaneous transhepatic cholangioscopy makes possible preoperatively the accurate and suitable diagnosis of atypism of the biliary tract epithelium.
    In addition, it is also useful for the diagnosis of the presence of cancer cells at the surgical margin and the degree of the cancer cell invasion.
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  • Toshio SHIMAYAMA
    1986 Volume 83 Issue 11 Pages 2411-2418
    Published: 1986
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    In order to elucidate the pathogenesis of chronic pancreatic disorders in hepato-biliary diseases, morphological and functional changes of the pancreas were studied in dogs with portal hypertension produced experimentally by supradiaphragmatic constriction of the inferior vena cava. Hepatic congestion and marked lymphstasis of the hepatic hilar and splanchnic area were inevitably observed. The results of exocrine pancreatic function test revealed a significant decrease in secretion volume and amylase output but not in maximal bicarbonate concentration and protein concentration in pure pancreatic juice. Microscopic findings demonstrated subcapsular and interstitial edema and dilated lymphatic vessels in the pancreas. Electron microscopy showed vesicular widening of rough-endoplasmic reticulum in acinar cells, widening of intercellular spaces of epithelial duct cells, widening of periacinar spaces with increased collagenous fibers in stroma. In stereological analysis, a significant changes of zymogen granules were observed in size and also in quantity. These findings are well correlated with decreased amylase output. However, as protein concentration in pancreatic juice be concerned, no significant change was observed despite decreased amylase output. This might be explained as that lymphatic fluid enter into the pancreatic ductal lumen through the widened intercellular space of the epithelial duct cells. These findings suggest that the chronic relapsing splanchnic lymphstasis may contribute to the pathogenesis of the chronic pancreatic disorders in patients with hepato-biliary disorders such as cholelithiasis and/or liver cirrhosis and may further contribute in part to idiopathic chronic pancreatitis.
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  • Toshikazu OHNUMA, Saburo NAKAZAWA, Yasuo NAITO, Kose SEGAWA
    1986 Volume 83 Issue 11 Pages 2419-2428
    Published: 1986
    Released: December 26, 2007
    JOURNALS FREE ACCESS
    In 16 cases of pancreatic head cancer comparative study was made between ultrasonically guided percutaneous pancreatograms and pathohistological findings of caudal pancreas.
    Type A showing regular beaded pattern of the main pancreatic duct had no cancerous invasion to the caudal pancreas. In advanced secondary pancreatitis the branches were more shortened. Type B demonstrating marked dilated main pancreatic duct with irregular beaded pattern and decreased branches in number showed cancerous invasion to the epithelium of the pancreatic duct. Type C showing the main pancreatic duct with rigid contour and without beading indicated the infiltration of the cancer to the parenchyma of the caudal pancreas. Type D displaying the marked dilated main pancreatic duct with filling defects corresponded to the intraductal proliferation and the epithelial cancerous invasion to the main pancreatic duct.
    It may be concluded that the US guided pancreatography in pancreatic head cancer provides with useful informations preoperatively regarding to secondary pancreatitis and tumor infiltration to the distal pancreas.
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  • S. INUZUKA, A. IRIE, I. IWAI, T. MATSUZAKI, T. EMURA, M. KOGA, K. KOFU ...
    1986 Volume 83 Issue 11 Pages 2429-2434
    Published: 1986
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • RESEARCHES IN LIPID METABOLISM
    Hironori TOKUMO, Toshio KAWAMOTO, Itaru HORIUCHI, Koki TAKATA, Kozo HA ...
    1986 Volume 83 Issue 11 Pages 2435-2439
    Published: 1986
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Masayuki HIROMOTO, Tetsu KATAOKA, Seikon CHO, Masatoshi KAWAMURA, Kazu ...
    1986 Volume 83 Issue 11 Pages 2440-2444
    Published: 1986
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Shuich SEKI, Kayo TANAKA, Masayoshi FUJISAWA, Susumu SHIOMI, Shinobu S ...
    1986 Volume 83 Issue 11 Pages 2445-2449
    Published: 1986
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Takefumi NAKAMURA, Fuminori MORIYASU, Nobuyuki BAN, Osamu NISHIDA, Ken ...
    1986 Volume 83 Issue 11 Pages 2450-2454
    Published: 1986
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Hideyuki HIRAISHI, [in Japanese], [in Japanese], [in Japanese]
    1986 Volume 83 Issue 11 Pages 2455
    Published: 1986
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Hirofumi NAKATA, [in Japanese], [in Japanese], [in Japanese], [in Japa ...
    1986 Volume 83 Issue 11 Pages 2456
    Published: 1986
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Yasuo TSUNEYA, [in Japanese], [in Japanese]
    1986 Volume 83 Issue 11 Pages 2457
    Published: 1986
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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  • Makoto KAWAI, [in Japanese], [in Japanese], [in Japanese]
    1986 Volume 83 Issue 11 Pages 2458
    Published: 1986
    Released: December 26, 2007
    JOURNALS FREE ACCESS
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