In this paper, the results of the studies on histochemical changes in experimental ulcers in cases of prolongation and accelerated healing were reported. In this study, the Clamping-Cortisone ulcer in rats were used. This experimental ulcer was made by the procedures mentioned before. In the histochemical studies, PAS, Alcian-blue, TBM, Aldehyde-fuchsin and Neutral red staining methods were used for examing polysaccharides in the tissues. Further, Gormori's and Azo staining methods were used for staining alkaline-phosphatase. The animals with C-C ulcer were divided into three groups, i.e. the C-C ulcer group treated continuously with cortisone during the experimental period, (named C-C-C group), the group treated with an anabolic steroid, 4Cl-TA (the C-C-A group) and the group treated with an anti ulcerous drug, basic aluminum sucrose sulfate (ulcermin), (the C-C-U group), The PAS reaction of the mucous content in the regenerative mucosa and connective tissue at the base of the ulcer developed remarkably in the early stage of healing in both the C-C-A and C-C-U groups, but was poor in the C-C-C group. The PAS reaction of the connective tissue was also remarkable in the two former groups, but was poor in the latter group. The Alcian-blue staining activities were the same as those of PAS, but fell in the latter stages of the healing of the ulcers. In the C-C-C group, the regenerative mucosal cells, the connective tissue, infiltrative neutrophile and capillary wall showed only a slight positive activity of alkaline phosphatase. In the groups treated with basic aluminum sucrose sulfate and 4Cl-TA, the regenerative mucosa and capillary wall in the connective tissue showed remarkable activity compared with that of the control group. From these findings, it appeared that the reasons why healing of the C-C-C ulcer was prolonged are an inhibition of mucous secreation from regenerative mucous cells and also poor development of polysaccharides and alkaline phosphatase in the connective tissues. On the centrary, the curative effects of 4Cl-TA and basic aluminum sucrose sulfate may be attributed to an accelerating activity of these agents on secretion of the mucous and on the formation of polysaccharides and alkaline phosphatase in the connective tissues.
Rats of Wistar strain weighing about 50g on an average were used throughout the present experiments. The rats were divided into 3 groups and they were fed on highprotein-highfat, high carbohydrate and low protein diets, respectively. On that special diets, rats fed for 4 months were classified as long-term feeding group. Otherweise, they, first fed on balanced diet for 4 months and then fed on that special diets for one week, were classified as short-term one. We analyzed the enzyme concentration obtained from duodenal collection pre-and postpancreozymin and secretin stimulation. From this analysis, an "adaptation" reactive pattern of the enzyme secretion to the main constituent of the diet had been found for amylase and trypsin, which were significantly increased when rats, of either longterm or short-term feeding group, were fed on highcarbohydrate and high protein diet. Lipase was not influenced by the high-fat diet. To a certain extent, the results mentioned above seem to explain one of the reasons why the difference in the clinical features of chronic pancreatitis between Japanese and Westerners may be referred to the varied pancreatic tissue reaction based on the different dietary habits.
With the purpose of studying the pathogenesis of peptic ulcer complicating liver cirrhosis, clinical and experimental investigation was done on gastric acid secretion. The effects of such surgical procedures as portacaval anastomosis and proximal gastrectomy on gastric acid secretion were studied on clinical materials. Gastric acid secretion was also studied experimentally in dogs with carbon tetrachloride poisoning and dogs with altered condition of hepatic circulation. These revealed; 1) The response of gastric acid secretion to histamine was doubled and prolonged following portacafal anastomosis in normal dogs. 2) These effects of portacaval anastomosis on gastric acid secretion were more pronounced when performed on dogs with carbon tetrachloride poisoning. 3) A 50%-increase in mean maximal gastric acidity was observed in dogs with carbon tetrachloride poisoning as well as dogs with hepatic vein constriction. 4) The histamine-stimulated gastric acid secretion augumented by portacaval anastomosis, carbon tetrachloride poisoning, or both was decreased by vagotomy added to them. 5) Following portacaval anastomosis, non-cirrhotic patients with intrahepatic portal obstruction developed a definite hyperacidity; the mean maximal acidity reaching as high as 90mEq/l. More prnounced augumentation of gastric acid secretion was observed when a portacaval anastomosis was performed on cirrhotics. 6) Following proximal gastrectomy combined with vagotomy and pyloroplasty, the gastric acid secretion was decreased by 30-59%, suggesting a lowered ulcerative diathesis. These findings would suggest that vagotomy and pyloroplasty have to be added to shunt operations, if the ocurrence of peptic ulceration is to be avoided, while proximal gastrectomy is secure in this regard.