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Masanori NISHIMURA, Michio SOWA, Yong-suk CHUNG, Hiroaki YOSHINO, Yasu ...
1989 Volume 86 Issue 4 Pages
843-850
Published: 1989
Released on J-STAGE: December 26, 2007
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We investigated both DNA histogram and the expression of carbohydrate antigens concerning to 168 patients with gastric cancer. Gastric cancer with type II DNA ploidy pattern showed a high incidence of vascular invasion and liver metastasis in the DNA analysis. Furthermore, ten patients with Stage II died of recurrence of gastric cancer within 2 years, of these nine patients showed type II or III DNA ploidy pattern. Then, gastric cancer with type II or III DNA ploidy pattern showed a high incidence of the expression of carbohydrate antigens, where they tended to distribute to cytoplasm and stroma. In conclusion, an analysis of nuclear DNA content appeared useful to predict prognosis in gastric cancer, in which type II DNA ploidy pattern showed the highest degree of malignancy, and both the expression of carbohydrate antigens and their localization possibly fill the role of parameter of the degree of malignancy.
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Ken HARUMA, Koji SUMII, Akihiko MORIKAWA, Naomi UEMURA, Akira INBE, Ma ...
1989 Volume 86 Issue 4 Pages
851-857
Published: 1989
Released on J-STAGE: December 26, 2007
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To assess the functional and morphological characteristics of the gastric mucosa of patietns with fundic hyperplastic polyps (FP), the determination of gastric acid secretion, serum gastrin levels, serum pepsinogen 1 (PG1) levels and histological examination were undertaken in 24 patients with FP, 34 with foveolar hyperplastic polyps (HP) and 62 controls, who had no gastric lesions. The following were the results of our investigation.
1) There were no differences between the patients with FP and the controls as to gastric acid secretion, serum gastrin levels, and serum PG1 levels. On the other hand, hypochrolhydria, hypergastrinemia and hypopepsinogenemia were common in those with HP.
2) Histological examination using gastric biopsy specimens showed almost normal gastric mucosa in patients with FP. However, severe atrophic gastritis of the fundus was common in patients with HP.
3) It was shown that there were definite differences between the patients with FP and those with HP with regard to the gastric function and morphology, although both types of gastric polyp were histologically classified as hyperplastic.
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Toshikazu YOSHIKAWA, Norimasa YOSHIDA, Mitunori YASUDA, Hiroshi ICHIKA ...
1989 Volume 86 Issue 4 Pages
858-864
Published: 1989
Released on J-STAGE: December 26, 2007
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Gastric hemorrhagic erosions were induced by the administration of platelet-activating factor (PAF). Gastric wall blood flow was decreased and thiobarbituric acid (TBA) reactants in the gastric mucosa were increased 10min after PAF injection. SOD and catalase reduced gastric mucosal lesions and TBA reactants, but had no influence on gastric blood flow. In polymorphonuclear leukocytes (PMN)-depleted rats, gastric mucosal lesions and TBA reactants in the gastric mucosa were reduced and gastric blood flow were increased. PAF induced the superoxide production from rat PMN in a dose dependent manner. These results suggest that oxygen-derived free radicals produced by PMN and lipid peroxidation may play an important role in the pathogenesis of gastric mucosal injury induced by PAF.
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Hirozumi OBATA, Tadao BAMBA, Shiro HOSODA
1989 Volume 86 Issue 4 Pages
865-875
Published: 1989
Released on J-STAGE: December 26, 2007
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The uptake of D-glucose, L-leucine and L-leucylglycine into intestinal brush border membrane vesicles isolated from rats was investigated. They were fed either oligopeptide (SP group) or amino acid (AA group) elemental diets for four weeks.
The results are as follows. As for L-leucine uptake, there was no significant difference in Km value between SP group and AA group. But Jmax value of SP group is significantly 1.7 times as large as that of AA group. As for L-leucylglycine uptake, there was no difference in Km value between the two groups, but Jmax value of SP group is significantly about twice as large as that of AA group. There is however, no difference in Km value and Jmax value between the both groups. These results indicate that L-leucine and L-leucylglycine transport carriers in the brush border membrane significantly increased in SP group compared with that in AA group, and that these carriers might be induced by oligopeptides as intraintestinal substrate.
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Tadashi TAKEDA, Shuhei NISHIGUCHI, Tetsuo KUROKI, Keiko ICHIBA, Yutaka ...
1989 Volume 86 Issue 4 Pages
876-881
Published: 1989
Released on J-STAGE: December 26, 2007
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Growth inhibition by interferon (IFN) was investigated in human hepatoma HLF cells by use of flow cytometry to study the cell cycle. INF-α or -β inhibited growth more than IFN-γ. Use of either IFN-α or -β and IFN-γ at the same time inhibited growth more than with any one kind of IFN, but use of IFN-α and -β together did not cause much inhibition. IFN inhibited growth by causing cells to accumulate in the S phase instead of moving on to the G
2 phase. Accumulation in the S phase was less with IFN-γ than with -α or -β. It increased with the combination of IFN-α or -β with IFN-γ, but not with the combination of IFN-α and -β.
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Part 2. Blood flow measurements in rats with liver cancer
Osamu MOTOHASHI, Shoichi KUSANO, Shigenari OHTA, Shigenobu NAKAYAMA
1989 Volume 86 Issue 4 Pages
882-888
Published: 1989
Released on J-STAGE: December 26, 2007
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The tissue blood flow (TBF) of primary liver cancer and normal live in 54 rats were measured following infusion of vasoactive agents into the celiac axis under continuous recording of blood pressure. Angiotensin II (AT) (1.0μg/kg/min) and Prostaglandin F2 alpha (PGF) (1.0μg/kg/min) were used. The former increased TBF of the tumor and blood pressure and the latter üreduced TBF of the tumor with no remarkable change of the blood pressure, but both drugs made little or no influence on TBF of the normal liver. Dibutyryl cyclic AMP (DBcAMP) (2.0mg/kg/hr) infusion increased TBF of the both tumor and normal liver with no significant change of blood pressure. PGF (1.0μg/kg/min) infusion after preinfusion of DBcAMP did not cause the decrease of TBF of the tumor.
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multivariant analysis on cases of national survey
Kyoichi INOUE, Hiroshi SASAKI, Toshifumi YASUYAMA, Kiyohiro HIGUCHI, M ...
1989 Volume 86 Issue 4 Pages
889-896
Published: 1989
Released on J-STAGE: December 26, 2007
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In order to predict prognosis and clinical course of BPC, theory quantification was applied and the discriminated rate was calculated concerning the cases of PBC national survey in Japan. We examined the prediction of three and five year's survival about all cases, the prediction of appearance of symptoms about asymptomatic PBC and that of jaundice about asymptomatic PBC and symptomatic PBC alone with pruritus. The useful items for the prediction of prognosis were serum bilirubin, albumin and the presence of esophageal varices at first medical examination. Fairly good discriminated rate was obtained on the prediction of three and five year's survival. However poor results were obtained concerning the prediction of appearance of symptons. In conclusion we can predict the prognosis of PBC based on clinical features.
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Naoki KUMAGAI, Kanji TSUCHIMOTO, Youji GOTOH, Kyouko TODA, Toshifumi H ...
1989 Volume 86 Issue 4 Pages
897-904
Published: 1989
Released on J-STAGE: December 26, 2007
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It was considered that nonresponsiveness to hepatitis B surface antigen was in part due to a failure in the antigen recognition (i.e., antigens' phagocytosis, processing and presentation by accessory cells). In order to enhance the capture of antigens, we investigated the effect of simultaneous administration of HBs antigen and anti-HBs IgG on antibody responses in vitro and in vivo. As a result, in vitro sensitization of mononuclear cells from anti-HBs-positive subjects with HBs antigen and anti-HBs IgG induced significant amounts of anti-HBs IgG production in 5 of 6 cases, whereas sensitization with HBs antigen alone failed to induce the antibody production in most cases. In vivo administration of HBs vaccine and anti-HBs immunoglobulin to non-responders to the vaccine triggered active anti-HBs antibody responses in 3 of 7 cases in vivo and in 5 of 7 cases in vitro.
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Rie HIRATA
1989 Volume 86 Issue 4 Pages
905-912
Published: 1989
Released on J-STAGE: December 26, 2007
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Serum concentration of aminoterminal type III procollagen peptide (P3P) and laminin have been shown as serum markers of liver fibrosis. In addition, liver membrane antibody (LMA) is suggested to play a role in the pathogeneis of chronic hepatitis. However, it is not known whether these serum markers are useful to predict the prognosis of chronic hepatitis. To test this, we measured P3P, laminin, and LMA in sera at the time of liver biopsies in 43 patients with chronic hepatitis who had serial liver biopsies more than two times during the 2-81 months (mean 25 months) follow-up period. Serum contents of P3P and laminin were measured by radioimmunoassay. Serum LMA was measured by radioimmunoassay according to the method of Thomas et al. The histological grading of liver fibrosis and of inflammation were scored according to Histology Activity Index by Knodell et al. Among thirty-two patients who had liver biopsies during 12-55 months, 16 patients showed histological progression on their latest liver biopsies compared with the first biopsies (Group 1). At the first biopsies, serum P3P levels were significantly higher in Group 1 than in 16 patients without histological progression (Group 2) (p<0.05). However, no difference were observed in serum laminin levels and in serum LMA between the two groups. Serum laminin levels were significantly correlated with the histological scores of fibrosis (comparison chisq=0.0089, df=2, p=0.995584) and inflammation (comparison chisq=21.4103, df=4, p=0.000263), respectively. In addition, serum P3P levels showed no correlation with the histological scores.
Serum P3P levels are suggested to be a useful serum marker to predict the prognosis of patients with chronic hepatitis. However serum laminin levels and LMA in sera have no predictive value; instead, serum laminin levels reflect the degree of liver fibrosis and inflammation at the time of serum sampling.
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Yasushi MAGAMI, Takeshi AZUMA, Zenji SASAKI, Hideto INOKUCHI, Akira AO ...
1989 Volume 86 Issue 4 Pages
913-915
Published: 1989
Released on J-STAGE: December 26, 2007
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It is well known that gastrin immunoreactive cells are observed in the fetal and postnatal rat pancreas. The role of the gastrin cells is unknown, however, it has been suspected that pancreatic gastrin may influence the development of pancreas and the gastrointestinal tract. The present study shows the localization of pancreatic gastrin cells and the ability of auto-proliferation of them in development. Gastrin cells were seen in 0-d to 2-wk-old rats in islet and in 0-d to 4-wk-old rats among exocrine cells. The ratio of gastrin cells to islet cells decreased in neonatal development and in rats older than 14-d gastrin cells were never observed in islet. Labeling indices of pancreatic gastrin cells after one injection of tritiated thymidine were 2.6% to 4.6% for 10 days after birth. It was suggested that gastrin cells have the ability of autoproliferation for 10 days after birth.
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Satoko OKAMOTO, Hitoshi OHNO, Yoshinori SAKAI, Hiroyuki TAGAWA, Kouji ...
1989 Volume 86 Issue 4 Pages
916-920
Published: 1989
Released on J-STAGE: December 26, 2007
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Yoshikane MAEDA, Takayoshi SEKIKAWA, Akihiro NOGUCHI, Fumio ARIHARA, T ...
1989 Volume 86 Issue 4 Pages
921-925
Published: 1989
Released on J-STAGE: December 26, 2007
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Mitsuru SAKA, Shigeaki ONO, Yoshitaka HIRAHARA, Masaki KUROSAWA, Ryuic ...
1989 Volume 86 Issue 4 Pages
926-930
Published: 1989
Released on J-STAGE: December 26, 2007
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Shinji TANAKA, Takahumi DOHZONO, Noriyuki ISHIDA, Manabu IMADA, Yoshih ...
1989 Volume 86 Issue 4 Pages
931-936
Published: 1989
Released on J-STAGE: December 26, 2007
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Tatsuji ISHIOKA, Ryouji MORISHITA, Hiroto MIURA, Tadashi SASAI, Tatsuo ...
1989 Volume 86 Issue 4 Pages
937-940
Published: 1989
Released on J-STAGE: December 26, 2007
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Shigeaki MORIURA, Yuji NIMURA, Naokazu HAYAKAWA, [in Japanese], [in Ja ...
1989 Volume 86 Issue 4 Pages
941-944
Published: 1989
Released on J-STAGE: December 26, 2007
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Takahiro MORI, Hiroshi TSUJI, Hidenori WATANABE, Koichiro MURAI, Kiyoh ...
1989 Volume 86 Issue 4 Pages
945-949
Published: 1989
Released on J-STAGE: December 26, 2007
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Takeshi MORIOKA, Eiki MATSUSHITA, Kazuo NOTSUMATA, Yutaka INAGAKI, Man ...
1989 Volume 86 Issue 4 Pages
950-954
Published: 1989
Released on J-STAGE: December 26, 2007
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Masamichi YAMADA, Yukiji OTANI, Takeshi AZUMA, Tsutomu AMAKAWA
1989 Volume 86 Issue 4 Pages
955-959
Published: 1989
Released on J-STAGE: December 26, 2007
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Tokimune SHIBATA, Tetsuo HAYAKAWA, Takaharu KONDO, Motoji KITAGAWA, Hi ...
1989 Volume 86 Issue 4 Pages
960-964
Published: 1989
Released on J-STAGE: December 26, 2007
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Takeshi YOSHIDA, [in Japanese], [in Japanese], [in Japanese], [in Japa ...
1989 Volume 86 Issue 4 Pages
965
Published: 1989
Released on J-STAGE: December 26, 2007
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Makoto OHARA, [in Japanese], [in Japanese], [in Japanese], [in Japanes ...
1989 Volume 86 Issue 4 Pages
966
Published: 1989
Released on J-STAGE: December 26, 2007
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