The consumption of soysauce and its loss per person per day were investigated. The amount of loss of soysauce was calculated from the amount of salt examined in the foods or/and liquid remainded in used dishes and pans.The samples were collected from thirty meals in ten days of a certain dormitory. It was observed that the average consumption of soysauce was 34.3 ml and the average ratio of its loss to the amount of the consumption was 9.6% with ranges from 3.9 to 17.9%.
An investigation was made on the diets among the 30 Katori settled families extracted arbitrarily from the 56 ones. Since each one of the settlers (who were the object of this survey) showed much more nutrients intake than the average one for farmer in the other parts of this country, the economic conditions of these families seem to be good as for settlers of the newly cultivated land. But as some of them (23.3%) took no animal proteins, it was assumed that there was an extreme difference between them. This could be also recognize from their consumption of seasonings, fishes, and its products. The mode of their living differs very much between them by the various, complicated conditions of location, soil, weather and farming plan; consequently, for the promotion of their diets, more systematic investigations must be undertaken in this settled village.
When vitamin A, absorbed previously on defatted soybean flours, was mixed with another materials at the beginning of manufacturing process, the loss of vitamin A in this “Miso” was 10% approximately after fermentation, and the decrease of vitamin A content during the storage for 2 months at 27°C. was very slight. Though the dispersion of vitamin A in “Miso” was not uniform at the early period of fermentation, the uniformity became high in the products owing to the tank-change process repeated several times during the fermentation and crushing. On the other hand, the direct addition of vitamin oil onto nonfortified “Miso” was carried out easily by the use of a suitable mixer, and the high uniformity of vitamin A dispersion was obtained in spite of the simple addition. The loss of vitamin A in this “Miso” was 2-3%, after 2 months storage.
The l-ascorbic acid content of green bean seedlings increased with the germination, when the plant was grown on the media with SH inhibitors, such as p-chloromercuribenzoate (PCMB), NaASO2, and CH2ICOOH, but this was reverse with SH compounds such as reduced glutathione (GSH), and cysteine. On the contrary the formation of l-ascorbic acid from D-glucurono-lactone, which is said to be the precursor of that vitamin during the germination was inhibited by the presence of SH inhibitors such as PCMB; but SH compounds such as GSH or cysteine increased this biosynthesis.
1) In the lumen of rat small intestine, D-glucurono lactone was converted to l-ascorbic acid, and this was promoted by the presence of SH compounds, such as glutathionine (GSH) or cysteine, but inhibited by P-chloromercuri benzoate (PCMB), NaAsO2, or CH, ICOOH. 2) In the case of ascorbic acid formation from d-glucose, Robinson-ester, or Harden Young-ester neither of SH compounds nor their inhibitors were observed to have this effect. 3) Yet the biosynthesis of l-ascorbic acid from Cori-ester was conspicuously accelerated by oxidized glutathione (GSSG) or PCMB, and inhibited by GSH and cysteine. 4) These results suggest us that the phosphorylation of D-glucose to Cori-ester is the first and direct pathway of the biosynthesis of l-ascorbic acid from D-glucose in intestine.
The mineral contents of the mother's milk, which was taken by the rachitic infants, differed greatly among the individuals, so that the definite tendency could not obtain. However, generally speaking, the amounts of the total calcium, dialytic calcium, phosphorus, sodium, and potassium were comparatively greater in the rachitic mother's milk, and those of the copper, and iron were a little smaller than those of the normal mother's milk.
Owing to the individual differences, the amounts of the minerals were scattered greatly in the feces of the rachitic infants who had taken the non-artificial feeding. However, the contents of the calcium, phosphorus, iron, and copper were generally greater and that of the potassium showed a reverse tendency, comparing with these contents in the feces of the normal infants.
硝酸加水分解法によるコリンのライネッケ定量法 (Ackerman法) は操作が簡易で殆んどあらゆる試料に適用出来る利点をもつが, 標準偏差が大きく, 結果がやや不正確である。著者はこの方法を検討し, n-propano洗灘時にNa2CO3, NaNO3が析出し, 結果を不正確にすること, 水解温度は800℃前後が適当であること等を知り, 更に他の条件についても検討を加えて, 食品及び飼料分析に一応適用の出来る方法を設定した。終りに終始御指導をいただいた学部長西本卯三博士及び中村定市講師, 資料を御送り下さつたU. S. Dep. of Biochem. and Nutrition, Dr. C. J. Ackcrman並びにBeckman Spectrophotometerの使用にあたり御援助をいただいた農学部栄養化学研究室の方々に深謝致します。