A technique to derivatize cellulose under mild conditions was devised. First, double bonds (allyl groups) were introduced into cellulose, and then the allylcellulose was converted to (2, 3-dibromopropyl) cellulose. The dibromo derivative was fairly stable and could be stored for more than 6 months at -20°C. Amino compounds reacted on (2, 3-dibromopropyl) cellulose at 37°C, and covalent bonds were formed between them. The cellulose derivatives prepared by this technique include |3-amino (or alkylamino)-2-bromopropyl|cellulose, |3-(ω-aminoalkylamino)-2-bromopropyl|cellulose, |3-(2-phosphoxy-ethylamino)-2-bromopropyl|cellulose, |3-anilino (or sulfanilino)-2-bromopropyl|cellulose. Cytochrome c
3 and flavin-adenine dinucleotide were combined to cellulose by this technique. The degree of substitution of the above derivatives depended on the type of amine, and was 0.38 when hexylamine was the ligand. Ligands having a thiol group was bound to cellulose at 100°C. Some of these compounds had affinity for copper ions and ionic dyes, ion-exchange capacity, and resistance to cellulase. The cytochrome c
3-combined cellulose had biochemical reactivity to transfer electrons to produce hydrogen gas from protons in a medium by the catalytic action of hydrogenase. The activity of 10mg of the cytochrome c
3-combined cellulose corresponded to 0.33nmol of free cytochrome c
3.
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