A polymer was prepared from poly(dichlorophospazene)[(−N=PCl2−)n] by substituting 86mol% of its chlorine groups with ethylamino groups and hydrolyzing the residual chlorine groups. This polymer, named PBEAP86, had film-forming ability and the film showed solubility in water at temperatures below 3°C. The polymer thus dissolved was gradually degraded in water. These properties of PBEAP86 were compared with those of one of the polymers prepared in the previous paper, which had a higher content of ethylamino group of 93mol%. The formation of complex structure of PBEAP86 with poly(acrylic acid) was also investigated by the help of measurements of infrared spectroscopy, differential scanning calorimetry, X-ray diffraction and tensile testing.
Hydroxyapatite-pulp composite fiber (HAPC) which has the structure of wood pulp fiber coated by hydroxyapatite (HAp) is referred to as HAp fiber. Tested pulps used in this investigation are commercially available soft wood breached kraft pulp (N-BKP) and hard wood breached kraft pulp (L-BKP). Preparing HAPC as starting materials, new type HAp sheet (25×25cm2, basis weight 75-100g/m2, HAp20∼40weight%) was relatively easily developed with paper making technique using tetragon type hand-sheet machine. HAp sheet has white color surface looked like commercial filter paper and high affinity to biomolecules, because the sheet surface is completely covered by HAp powders. This new sheet making technique using HAPC is simpler and cut down the price in comparison with conventional technique for making inorganic filler paper with the retention aids. HAp sheet has similar mechanical strength to commercial filter paper 51B and is easy to manipulate. HAp 36.8weight% sheet LP-2 adsorbed BSA 13mg/(g of sheet) or salmon sperm DNA 4.6mg/(g of sheet).
In order to produce bleached pulps in high yields from softwoods, the behaviors of cooking and bleaching was investigated. In the sulfite-formaldehyde-anthraquinone (SFQ) cooking of akamatsu (Pinus densiflora), the high-yielded chemical pulp with 49.5 of kappa number was produced in a 54.5% yield. However, the rejects yield was much higher than that of the ezomatsu (Picea yezoensis) wood pulping. Akamatsu wood was shown to be appropriate for polysulfide-anthraquinone (PS-AQ) pulping but be inappropriate for the SFQ method. Although kraft pulp could be bleached to high brightness in the nitrous acid pretreatment and hydrogen peroxide followed by chlorine dioxide and peroxide bleaching (NP1DP2) process, the brightness of the SFQ pulp did not increase very much. In the ozone pretreatment and sulfite delignification followed by ozone and peroxide bleaching (ZS-Z1P) process, the SFQ pulp could be bleached to high brightness with acceptable viscosity. However, the yield decreased largely. The AEOPHDP process standing for the acid pretreatment for the manganese reduction, oxygen-pressurized hydrogen peroxide, hypochlorite, chlorine dioxide, and peroxide bleaching process was shown to be effective for bleaching of PS-AQ pulp to high brightness with acceptable viscosity, but be ineffective for bleaching of SFQ pulp.
New type cell culture bed apatite sheet was made from Hydroxyapatite-pulp composite fiber (HAPC) and polyethylene fiber by the method as wide as possible to open surface area using paper making technique. It has become apparent that apatite sheet has good structure for cell culture bed. The sheet has many 10-200μm diameter pores which were formed by ribbon tape shape HAPC. And pore had suitable apatite surface on which mammalian cell grow. These pores linked from top side of sheet to bottom side, and its surface area was 0.32m2/g. CHO-K1 cell were statically cultured in high density on apatite sheet bed which was set in 60mm plastic dish with 7ml Ham F-12 medium includeing 10%FBS. Cell culture bed apatite sheet is expected to be used extensively for cell culture procedure by researcher working in biological industry.
A gram-negative bacterium strain AS assimilating disodium terephthalate as the sole carbon and energy sources was isolated from soil. The strain AS grew aerobically and produced a yellow metabolite (λmax=405.6nm) with growth. The metabolite fluoresenced a light blue color by irradiating ultraviolet light of wavelength 365 nm with UV lamp. The metabolite was stable on heating at 100°C. The molecular weight was below 10, 000.