The two feeding experiments were simulta_??_eously carried out with four mature milk cows which had been averaged 10kg milk yields a d 500kg live weight.
One group with two cows received a ration composed partly of Tapioka ampas 2.26kg and mixed concentrates 0.68kg as the substitutes for the beet Pulp 3.11kg per 1000kg live weight and the other had Pineapple bran 2.72kg and mixed conc_??_ntrates 0.68kg in place of them too, being equal to one another in the dig stible pure protein and starch value.
The quantitative cha_??_ges of milk and milkfat etc. produced by two groups during the feeding of different rations had been studied.
The conclusions that may be drawn from thiswork are; the feeding of both Tapioka ampas mixture and Pioeapple bran mixture so prepared that th_??_ir lack of protein are balanced by high protein mixture which almost its protein is from ground soy bean oil cakes, shows on an average higher increase in milk and milk fat than the feeding of beet pulp as the control.

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About 130 materials of various stages have been used in this study. The results of observations are as follows:
1) The parenchymatous cells of the pineal body are derived from the ectodermal ependyma cells, while the stroma of the body are formed by the mesenchyme together with the neuroglia and acidophilic granule cells.
2) The parenchymatous cells of the pineal body contain the basophihc granules which have been discharged in various functional stages of the cells. These observations have support the idea that the cells are the secretory type.
On the other hand. grocups of special type of degeneration cells hitherto undescribed) aru found scattered among the ordinary parenchymatous cells They are finally change themselves into the lipoidal substance.
Thus, the pineal body of the fowl seems to have both secretory types of merocrine and holocrine gland.
3) The secondary follicular formation of the cells has oeen observed. The formation begins with the degenetation of the cells which lie in the center of the cell cluster. The cells surrounding the follicular cavity change into the peseudo. ependyma.
4) The pigment cells (which supposed to contain the lipopigment) appear among the ord. inary parenchymatous cells of the pineal body, in the later developing stages of the embryos and maintain their existence throughout their lives hereafter.

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1 A modification of the method of T OMAS et al. for the determination of arginine in protein hydrolysates was discussed.
2 It was thown shat the amount of tyrosine in the samples and the varying amounts of hypobro. mite used for the estimation were highly important factors contributing to consistent results.
3 The arginine value obtained for a vacum dried lactoprolactin, was 3.02 per cent by the modification of the ThomAS methed and was lower than the values of beef and sheep prolactin reported by EVANS et al.

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In the ejaculated semen of the stallion there can be found some quantities of viscous, gelati. nous material, of which characters or source qu'teunknowa. We have studied these problems andobtained results as follows.
1. Quantity of the gelatinous material varies considerably in different seasons. In the bree. ding season, it is seen in 83.9% out of 205 cases of 15 stallions. The average volume is 63 cc, the maximum being 300cc. The average volume ratio of the material for total semen is 42.0 to 93.8%. The volume deviates in a wide range according to the breed or individua. lity of stallions and also to the, time of collec. tion. In the no.a.breeding season, however, the material can not or scarcely found in the semen. Sexual stimulation is also one of the deciding factors as for the volume of the fraction.
2. The colour, turbidity or smell, of the material are almost the same as of the fluid semen, except the viscosity being, extremely higher. PH of the material is higher by 0.1-1.0, in average 0.38 than that of the fluid.
3. Many spermatozoa are contained in the mat. erial, sperm concentration per 1/1, 000 mm³ Leing 16 to 160, in average 74.5 and total number of sperm being 240 to 16, 020 million, in average 5, 716milion. Motility of the sperm in the freshly ejaculated fraction is almost the same as in the fluid fraction, while the activity is much slower than that of the latter.
4. It seems probale that the material comes from the seminal vesicle, being vigorously secreted

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The material is the ovaries of a jenny ass assumed to be in the pro-oestrus. One ovary had two follicles before and after the ovulation, while another one was in the resting period. They were investigated anatomically and histologically. The ovarian changes, traced by rectal palpation in the previous report. could be ascertained.
1. The sexual organs of the jenny are much larger than the mare's in the proportion with the body: the ovary is remarkably large, but the relative position between the genital tracts and the ovary is the same as the mare's.
2. Two follicles in one ovary can develop in the same time and they ovulate successively with short interval.
3. The ovulation groove is situated in about the center of the inner curve of the ovary and except the groove surface, is covered with the tough peritoneum. The ovulation takes place at the ovulation groove only.
4. The relation between granulosa cells or theca interna and the maturation of the follicle is almost the same as that of mares.
5. Anatomical and histological observations of the follicle immediately after ovulation are almost the same as that of the mare, that is, thin whitish-yellow layers are seen in its rims and the inner cavity is filled with bloody materials. It is ascertained that the formers develop to the corpus luteum rapidly.
6. It seems that the origin of the luteal cells is the theca interna. In the large follicle just before the ovulation, theca interna cells are rem. arkably enlarged and develop to the so-called "theca luteal cells". After the ovulation, theca interna sends forth rapidly luteal cells into the cavity of the ruptured follicle. The appearance in which granulosa cells develop into the corpus luteum could not be obtained from this material.

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The effect of several antibiotics on the spermlongivity of the rabbit semen which was preserved in room temperature was observed. Acetosulfamin, penicillin G, Streptomycin and Streptothricin group substances (No.36 substance, Seki-2, Seki-3, and Seki-7 substances) were used as the antibiotics.
The semen-diluter was compenoated with the phosphate buffer solution to three groups of pH 7.6, 7.0 and 6.5 respectively. The antibiotics were contained in each these groups of preserved semen as follows. The density of Acetsulfamin was mede up 0.5mg×10^-1, 0.5mg×10^-2, 0.5mg ×10^-3, 0.5mg×10^-4, and 0.5mg×10^-5 per ml of diluted semen, and those of the others were made up 1mg 10^-1, 1mg×10^-2, 1mg×10^-3, 1mg ×10^-1, and 1mg×10^-5 per ml. As the control spermlongivity of the preserved semen was observed without carried on in sterilized condition, so there were no effect of bacteria.
The average spermlongivities cf diluted semen without respect to the density of antibiotics were shown in the table.
1) In the case of pH 7.6, the sperm lived longer in Penicillin G, streptomycin, Seki-3 substance and No. 36 substance in control. In Seki-2 substance, the lengivity was equal to control, 86 and in Acetsulfamin or SEKI-7 substance, it was shorter than in control. Particularly, SEKI-7 substance had a toxity for spermatozoa.
2) In the case of pH 7.0, the longest spermlongivity was observed in SEKI-2 substance. In Acetsulfarmin, No. 36 substance and Streptomycin it was longer than in control, , In Penicillin G and Seki-7 substance, it was SRELATIVELY-3 shorter hanin control, and the shortest was in SEKI-3 tsubs- tance.
3) In the case of pH 6.5, all the spermlongivity of the preserved semen containing these antibiotics were shorter than the control, and it was remarkably shorter in SEKI-3 and SEKI-7 substance.
4) Feing compared one another those three kinds of pH the spermlongivity was considerably longer in pH 7.0 than in the cases of pH 7.6 and 6.5. The shortest was in pH 7.6.
5) The antibiotics used, except SEKI-7 substance had no toxity for spermatozoa in the case of pH 7.0 and 7.6, and SEKI-3 substance showed, however, a little toxic effect on spermatozoa in the case of pH 7.0.
Those results above mentioned suggest that six kinds of those antibiotics, namely Acetsulfamin, Penicillin G, Streptomycin, No. 36 substance, Seki -2 substance and Siki-3 substance may be able to applied to artificial insemination of domestic animals, if the condition of the medium is considered.Particularly, the application of Streptomycin, No. 36 substance and Seki-2 substance, of which actions in keeping the motility of spermatozoa suitably appear in the neutral or the basic, may give a satisfactory result

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Tow Holstein steers., three young Saanen goats (tow males, one female) and young Middle Yorkshire hogs were rangel for four month (from July to Oct., 1945) on the natural forest pasture of Uryu Experiment Forest of Hokkaido University, and their growth rate was measured monthly.
1) Steer No. 1 which was 7.5 month of age at the begining of this experement gained 830g live weight daily, and No. 2 (5.5 month old), gained 662g on each average during the whole period. The authers recognize that the better growing results. were obtained by rainging on the natural pasture mainly consisting of bamboograss rather than the normal daily gain of 568g corresponding to their age.
2) The growth rate of three experimental goats also exceeded the nolmal namely the daily live w e ight gain of the female goat was 153g and 91 those of male goats were 110g and 154g, while the standard daily live weight increase of corresponding age are 93g in female, 102g in male.
3) On the contrary the piglings could not reach to the normal growth for reason of too poor digest abililty for coarse fodder to derive their neeessary nutrients thoroughly from such pasture grasses. Their daily increase were 194g and 238g which were inferior to the normal gain of 370g.
4) At the end of the experiment the typical ones of each group were sraughtered to examine each slaughtering percentage. The resurts of the slaughtering test are as follows: Slaughtering percentage of total percentage meat to live weight Steer No.1 51.21% 28.19% Male goat No. 2 30.17% 15.07% Hog No. 2 66.77% 47.79%
From the above data it is recognized that the steer No 1. was just medium flesh condition of Holstein cattle, but the pigling No. 2 was not desirably plump.
It may be concluded from this study that the natural forest pasture mainly consisting of bamboo-grass in Hokkaido can be utilized for fattening catlle and oat.

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1) For tht purpose of improving Berkshire swine Sus scrofa var. domesticus Brisson) in Ka. goshima prefecture, Japan, the physical characters of 154 swine, aged 1.5 or more, were biometrically studied.
Ten characters of the body were measured, and various biometrical values, the mean value, standard deviation, variation coefficient, correlation coefficient and regression equation were calculated.
The normal type shown in the tabl was determined in the usualway.
2) It was observed that 50.65% fitted into the normal type.

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