P. salmoneostramineus was cultivated on unsterilized rice and wheat straws which were ground or chopped to investigate the effect of non-sterilization on the fiber composition and digestibility of the straws. Rice and wheat straws were chopped to 3 to 5cm length or ground to 1mm in size and then stuffed into a 450ml mayonnaise bottle. The grain spawn of
P. salmoneostramineus was inoculated to unsterilized straws. As a control,
P. salmoneostramineus was inoculated to sterilized straws. Wheat and rice straws were cultured at 20°C under semi-anaerobic conditions for 30 and 50 days, respectively. Then they were cultured for 30 days under aerobic conditions to generate fruit-body. The mycelia of
P. salmoneostramineus spread 30 days after inoculation in all straws except in unsterilized chopped rice straw. The contents of organic matter and cellulose tended to be lower in unsterilized straws than those in sterilized straws, and those of the hemicellulose and lignin in unsterilized straws tended to be higher than in sterilized straw. Higher values of
in vitro organic matter digestibility (OM digestibility), neutral detergent fiber digestibility (NDF digestibility) and gas production were obtained in sterilized straws and unsterilized ground wheat straws as compared with intact rice and wheat straws, and lower values were obtained in unsterilized chopped straws as compared with intact straws. Furthermore, OM and NDF digestibilities of unsterilized ground rice straw were greater than those of intact rice straw, but the gas production of unsterilized ground rice straw was lower than that of intact rice straw. To improve
in vitro digestibility of straw cultured with
P. salmoneostramineus under unsterilized conditions, it is important to crush the straw and suppress the weed-fungi activity, in order to have a better nutrition supply for basidiomycetes.
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