In order to investigate the contribution of plasma palmitic acid to the increase of total energy production in a cold environment, the metabolic parameters of plasma palmitic acid were measured in fed sheep on the 4th day after exposure to both thermoneutral (20°C, 70% relative humidity) and cold (0°C, 70% relative humidity) environments.
The mean turnover rate of the carboxy carbon atom (1-C) of plasma palmitic acid in acold environment (0.039±0.007mg/min•kg
0.75) was four-times higher than that in a thermoneutral environment (0.010±0.009mg/min•kg
0.75). The percentage of 1-C of palmitic acid oxidized to CO
2 in the total turnover rate increased fourteen-fold from 3.3 ±1.3 in the thermoneutral to 45.1±16.8% in the cold. The percentage of expired CO
2 derived from 1-C of palmitic acid in the total CO
2, therefore, increased twenty six-fold from 0.006±0.005 in the thermoneutral to 0.156±0.032% in the cold. The total energy production increased 2.5-fold from 3.06±1.01 in the thermoneutral to 7.66±2.63 kcal/hr kg
0.75 in the cold. The energy originating from 1-C of palmitic acid oxidation increased forty six-fold from 0.0004±0.0003 in the thermoneutral to 0.0184±0.0065 kcal/hr.kg
0.75 in the cold. Therefore, the percentage of the energy derived from 1-C of palmitic acid increased six-fold from 0.04±0.05 in the thermoneutral to 0.24±0.08% in the cold.
The data obtained from [1-
14C] palmitic acid were corrected to estimate the values for a whole molecule of palmitic acid. The mean turnover rate of plasma palmitic acid, the percentage of palmitic acid oxidized to CO
2 in total turnover rate, the percentage of expired CO
2 derived from palmitic acid in the total C02, the energy originating from palmitic acid oxidation, and the percentage of energy derived from palmitic acid were 4, 15, 30, 60 and 27 times higher in the cold than those in the thermoneutral, respectively. These results indicate that the turnover rate of plasma palmitic acid in a cold environment was greater than that in a thermoneutral environment, and that the contribution of plasma palmitic acid to the increase of total energy production increased significantly in fed sheep exposed to a cold environment.
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