The presence of iron in the rumen mucosa of cattle was examined by electron probe X-ray microanalysis. A great deal of iron was detected in the dark brownish coloured mucosa obtained from adult cattle, while a very small quantity of iron was found in the uncoloured mucosa obtained from fetal cattle. Therefore, it is assumed that the intensity of dark brownish colour depends on the level of iron in the mucosa. In agreement with histological demonstration, iron was localized in the pigments deposited in the epithelium of mucosa. Out of 16 elements (Al, Br, Ca, Cl, Co, Cr, Cu, Fe, I, K, Mg, Mn, Os, P, S and Zn) analyzed, calcium, iron, kalium, manganese, osmium, phosphorus and sulphur were detected distinctly in the dark brownish coloured mucosa. Next to iron, phosphorus was found in relatively large amounts and its distribution almost agreed with that of iron. Accordingly, iron may be combined with phosphorus and contained in the pigments in the form of iron-phosphate. Since most of pigments appeared as masses which attached to the outmost layer of epithelium and were negative for melanin test, it is thought that the pigments are not producted in rumen tissue but originated in rumen contents.
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