The hemoglobin (Hb) types detected by starch gel electrophoresis, starch gel electrophoresis in 6M urea and iso-electric focusing were mutually compared and analyzed. At the same time, the gene and haplotype frequencies of Japanese native horses were also studied. The following results were obtained: 1. The A, A/a and a phenotypes of the Hb types according to BRAEND and STORMONT's classifcation
1) coincided with the 1, 1/0 and 0 types of the Hb-α (dup.)locus, respectively. Accordingly, the Hb
A and Hb
a alleles were considered to be the same marker gene as the Hb-α
1 and Hb-α
0 alleles. The haplotype A characterised by means of IEF was then specifically observed in individuals with the Hb
a (or Hb-α
0) allele. 2. The Hb
a (or Hb-α
0) gene and haplotype A were observed in four breeds; Hokkaido-, Kiso-, Tokara-and Yonaguni-native horses of the Japanese native horses. The highest frequency was 0.533, for Tokara-native horses. 3. With regard to haplotypes, Noma-native horses only had type B
I/B
I. The haplotype B
I was not observed in Tokara-native horses, but the haplotypes B
II and A were observed, being 46.7 and 53.3%, respectively. However, haplotype A was not recognized in Misaki-native horses, although the frequencies of haplotypes B
I and B
II were 43.8 and 56.3%, respectively.
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