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Volume 38, Issue 6
Displaying 1-6 of 6 articles from this issue
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Hiroshi SUTOH1967 Volume 38 Issue 6 Pages 233-244
Published: June 25, 1967
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSDownload PDF (961K) -
3. On the Fractionation of Conjugated Lipids in Cow's Milk by Thin-Layer ChromatographyToru DOI, Shigeru MORI, Kazuo MINO1967 Volume 38 Issue 6 Pages 245-251
Published: June 25, 1967
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSPhospholipids and glycolipids in milk were fractionated by silica chromatography and florisil chromatography, and each fraction was further fractionated thin-layer chromatography.
According to the extracting condition in this experiment (milk 1500ml, viz. acid-precipitation, extraction with ethyl-alcohol: ethyl-ether: chloroform=3:1:1, silica for chromatography 50g, celite 25g, column 2.3×50cm, elution with chloroform 300ml and chloroform: metylalcohol=1:4 400ml), extraction of sphingomyelin was not possible.
Consequently the extract can not be regarded as perfect conjugated lipids, but it was recognized that the extracted lipids consisted of five phospholipids and three glycolipids on thinlayer chromatography.
Namely lecithin, cephalin and three unidentified constituents were detected as phospholipids and two of the unidentified phospholipids were ninhydrin + (plasmalogen, lysophosphatidylethanolamine ?) and one was ninhydrin - (phosphatidyl-inositol?).
The ammonium molybdate reagent (I) (3g ammonium molybdate dissolved in 25ml water and mixed with 10ml of 1N HCL and 5ml of 60% HCL) which was devised by the authors was better in coloration and stability than other coloring reagent reported for detecting conjugated lipids except for the defect that the reagent should be prepared every times before using.
The anthrone reagent (1g anthrone dissolved in 50ml conc H2SO4 and mixed with 25ml water) which was also devised by the authors gave bluish green color for glycolipids and enabled the detection of the glycolipids which were mixed with phospholipids.View full abstractDownload PDF (414K) -
X. An Influence of Level of Nitrogen Fertilization and Stage of Maturity on Chemical Composition and Feeding Value of Teosinte Forage (Euchlaena mexicana Schrad.)Takashi MIAKI1967 Volume 38 Issue 6 Pages 252-256
Published: June 25, 1967
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSIn the present study, the influence of 2 levels of nitrogen fertilization viz. 48kg N (high level) and 12kg N (low level) per 10a, on the chemical composition, digestibility as well as digestible nutrients of teosinte forage was determined in connection with the stage of maturity viz. early vegetative (in August), middle vegetative (in September), late vegetative (in October), boot and head stages.
In order to estimate the digestibility of teosinte forage, male rabbits were used and the forage was fed in fresh form cut in small pieces to them.
The results obtained were summarized as follows:
1) Due to the high level of nitrogen fertilization, the content of crude protein increased during the stage of early vegetative to late vegetative whereas NFE content decreased.
2) The digestibility of crude protein increased due to the high level of nitrogen fertilization throughout all the stages of growth except middle vegetative stage, while a significant increase (P<.05) was observed at the late vegetative and boot stages.
3) Due to the high level of nitrogen, DCP content significantly (except P<.05 at boot stage, P<.01 being at other stages of growth) increased during all the stages of growth, while TDN content did not increase at all growth stages.
4) Regardless of the level of nitrogen fertilization, DCP content declined as the growth stage progressed, having shown lowest value at the head stage, and especially in high level of nitrogen fertilization it remarkably declined.
TDN content showed a little decline at the middle vegetative stage and then rose gradually.
5) The content of crude protein was highly correlated with the DCP content, its correlation coefficient having been 0.963 (P<.001).View full abstractDownload PDF (329K) -
Masataka YUHARA, Hiroshi WADA1967 Volume 38 Issue 6 Pages 257-261
Published: June 25, 1967
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSThe study was carried out to compare the growth promoting effect of hexestrol dicaprylate on broiler with that of diethylstilbestrol and hexestrol. The influence of vehicle on the effect of the compound was also tested in this study.
Two hundreds and ten newly hatched chicken of the Kimber 44 broiler were used. These birds were divided into seven groups of 30 birds each, consisting of 15 males and 15 females, and raised for 7 weeks and then killed.
At 4 weeks of age, six groups were injected with 7.5mg, 12.5mg of hexestrol dicaprylate in oil, 7.5mg, 12.5mg of hexestrol dicaprylate in paste, 7.5mg of hexestrol in oil and 7.5mg of diethylstilbestrol in oil singly at the back of the head. One group served as control.
Hexestrol dicaprylate showed a more rapid and higher growth promoting effect than hexestrol and diethylstilbestrol when compared on the bases of the same dose. In regard to the dose of hexestrol dicaprylate, the weight gain was fairly higher in the 12.5mg group than in the lower dose group in both vehicles respectively. The effect of the hormone on weight gain was prolonged in paste than in oil.
Dressing data showed an increase of abdominal fat deposit and of weight of the uterus and oviduct in the hexestrol dicaprylate injected group in comparison with the groups injected with diethylstilbestrol or hexestrol.View full abstractDownload PDF (262K) -
IV. Fatty Aldehyde Composition of Plasmalogens in Cattle Muscle TissuesTakeo NAKANISHI, Kyozo SUYAMA1967 Volume 38 Issue 6 Pages 262-268
Published: June 25, 1967
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSThis study aimed at elucidation of fatty aldehyde composition of plasmalogens in cattle muscle tissues as influenced by the location of muscle, degree of fatness andage. The content of fatty aldehyde in fatty acid, fatty aldehyde mixture of phospholipids and the component fatty aldehydes of plasmalogens in M. masseter, M. longissimus dorsi and M. biceps femoris of each carcass of lean cattle, fattened cattle and calf were determined by gas liquid chromatography.
The results obtained were as follows:
1. In the cases of lean and fattened cattle, the content of fatty aldehydes was higher in M. masseter than in M. longissimus dorsi and biceps femoris, while in the case of calf any significant variation in fatty aldehyde content did not occur depending upon the location of muscle tissue. Further, the muscle tissues of fattened cattle showed lower percentages of fatty aldehydes in general as compared with those of lean cattle.
2. Palmitaldehyde, stearaldehyde and olealdehyde accounted for about 80% of the total aldehydes found in muscle tissue plasmalogens. Of them all, the palmitaldehyde was the highest in the percentage content.
3. In lean and fattened cattle, M. masseter plasmalogens were higher in the percentage of palmitaldehyde and lower in the percentage of stearaldehyde than other muscle tissue plasmalogens. In phospholipids, however, the variation in fatty aldehyde composition was narrower than that in fatty acid composition, depending upon the location of muscle tissue.
4. There was a considerable variation in the fatty aldehyde composition of plasmalogens among the lean cattle, fattened cattle and calf muscle tissues, i.e. calf muscle plasmalogens were higher in the percentage of olealdehyde (10-13%) and lower in that of stearaldehyde (11-15%) than lean cattle muscle plasmalogens (C1=18=4-7%, C18=25-30%) and fattened cattle muscle plasmalogens (C1=18=7-9%, C18=18-25%).View full abstractDownload PDF (430K) -
XII. The Feeding Value of Corn, Sudangrass, Sorgo and TeosinteKuniyasu TAJI, Kazuo KOYAMA1967 Volume 38 Issue 6 Pages 269-274
Published: June 25, 1967
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSIn order to determine each feeding value of the different sowing time and that of the different cutting frequency of representative summer forage crops, the digestion trials of corn sown between March and August, sudangrass in April, sorgo in April and May and teosinte in May were conducted.
Materials for the digestion trials that were collected by the same quantity of hay (water content 20%) on each cutting date in the cutting periods extended from 25 to 35 days in principle were mixed and fed for rams.
The results were as follows:
1. In the feeding value of corn, the minimal value was shown by the crop sown in March and the maximum value was shown by it sown in April. Now, the crop sown in June grew rapidly, but that feeding value was less than those of the crops sown in April, July and August.
2. The feeding value of sudangrass in the same stage of growth did not show significant difference, and so that had no relation with the cutting frequency.
3. In the feeding value of sorgo cut between the heading stage and the milk stage, that of the crop cut from late July to early September showed the best result.
4. In the feeding value of teosinte, that of the first cut (the vegitative stage) was richer than that of the second (the heading stage). In the crop sown in May, the feeding value of it by early sowing was richer than that by late sowing.View full abstractDownload PDF (394K)
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