All issues
Volume 38, Issue 8
Displaying 1-7 of 7 articles from this issue
- |<
- <
- 1
- >
- >|
-
Chuhei YAMAUCHI1967 Volume 38 Issue 8 Pages 321-327
Published: August 25, 1967
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSDownload PDF (630K) -
The Effect of Selection and Inbreeding on the Ribonucleic Acid Content of Mammary Gland in MiceYung-Yi SUNG1967 Volume 38 Issue 8 Pages 328-333
Published: August 25, 1967
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSIt is clearly established that the milk yield can be affected by the quantity and quality of the parenchyma of mammary gland. It is also known that the desoxyribonucleic acid (DNA) content of the mammary gland can indicate the quantity of the parenchyma of mammary gland and that the intensity of protein formation can be expressed by the ribonucleic acid (RNA) content which may correlate with the quality of the parenchyma of mammary gland.
The present experiment was undertaken to study the changes of the nucleic acid content in the mammary gland at the peak-period of lactating mice in order to make advance in future selection experiments.
All the mice used were CFW strain. Fourty one female mice were divided into 4 groups at random and sacrificed on 10, 12, 14 and 16 days postpartum, respectively. Using dry powder of the abdominal-inguinal mammary glands, the fraction of nucleic acid was extracted by the method of SCHNEIDER. DNA and RNA contents were determined following a YAGI's modification of DIscaa and MEJBAUM, respectively. Litter size was adjusted to 8 pups per mother at birth and to 6 pups on 5 days postpartum. The body weight changes of mother and litter were recorded. Weight of fresh and dried fat free tissue (D.F.F.T.) of mammary gland were measured and total DNA-P, total RNA-P contents and the ratio of RNA-P/DNA-P were estimated. The results obtained were summarized as follows:
1) Mean and standard error of the weight of mother, weights of fresh and D.F.F.T. of mammary gland, total DNA-P, DNA-P/mg D.F.F.T., total RNA-P, RNA-P/mg D.F.F.T., RNA-P/DNA-P ratio and pup weight are shown in table I. There were no significant differences among the days of lactation in those characters since the variances were fairly large.
2) The deviation of those characters at 12 days postpartum was smaller than these of other days of lactation.
3) The coefficient of correlation between total DNA-P and D.F.F.T. was not significant. 4) The curve of RNA-P/DNA-P seemed to be parallel with the pup weight gain (Fig. III).
5) The total RNA-P remained higher during 10 to 12 days than during 14 to 16 days, but the total DNA-P indicated a tendency to decrease gradually from days 10 to 16 of lactation (Fig. I, II.).
6) Highly significant coefficients of correlation were found between RNA-P/DNA-P and litter weight gain (r=0.45). When. the effect. of days of lactation was held constant, its partial correlation coefficient was also significant (r=0.41), (Table II).
7) On improving milking ability, high DNA, RNA and RNA/DNA ratio are necessary and from the evidences obtained in the present experiment of small variation in measures at 12 days postpartum, it is considered that the selection of mice on milking ability with the ratio of RNA/DNA on 12th day of lactation might be, useful.View full abstractDownload PDF (344K) -
Shichiro SUGAWARA, Saburo TAKEUCHI1967 Volume 38 Issue 8 Pages 334-338
Published: August 25, 1967
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSThe tubal secretion rate and the chemical composition of tubal fluid were investigated in the rabbits which had been treated with gonadotrophins to induce the estrus, as well as. at the time of spaying and with spayed one receiving estrogen and progesterone.
In the rabbit treated with gonadotrophins, the secretion rate and the contents of glucose, lactic acid and volatile fatty acid were highest when she was in heat. Glucose and volatile fatty acid increased in the progestational stage, slightly.
After castration, the rate of tubal secretion decreased to about two-third of diestrous level. The secretion rate and the content of chemical components of the castrates increased following estrogen treatment. After the administration of progesterone, glucose and volatile fatty acid were found to increase in their concentration.
These changes in the tubal secretion and the amount of the chemical constituents of the fluid from the rabbit receiving the ovarian hormone were similar to the results obtained in the gonadotrophin injected animals.View full abstractDownload PDF (361K) -
1. The Amount of Nicotinic acid in Rumen Content and the Chemical Form of Nicotinic Acid SubstancesNaomi TAKAHASHI, Koichi KUBO, Junnosuke OSAWA1967 Volume 38 Issue 8 Pages 339-344
Published: August 25, 1967
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSTwo goats, male and female were fed on hay and concentrate for a week, and then the rumen contents were collected through fistula. The amounts of rumen nicotinic acid were estimated by the method of bioassay using Lactobacilius arabinosus.
As the results the authors recognized that the amount of 25 to 40γ of nicotinic acid was contained in one gram of dry matter of rumen contents which were collected before feeding in the morning. This value increased after feeding of hay and concentrate under such conditions.
Then the water extracts of cell of rumen bacteria and protozoa were prepared, and the chemical form of nicotinic acid substances existing in these fractions were examined by the method of paperchromatography with the solvent of butanol and 1.5N of ammonia water.
In the fraction of bacteria, nicotinic acid was always detected as the orange-yellow spot of Rf value of 0.24 by KöNIG colour reaction with the reagent of cyanogen bromide and paminoacetophenone but no other spot was detected. In the fraction of protozoa and liquor from which the microorganisms being excluded, no spot whatsoever of KONIG colour reaction was detected. But five or six fluorescent spots with the methyl ethyl ketone and ammonia under the irradiation of ultra-violet ray were recognized in the three fractions of bacteria, protozoa and liquor through all the experiments.
According to these results it was concluded that as the pyridine compound only nicotinic acid existed in the bacterial fraction and five or six nicotinic acid substances which belong to the pyridinium compound existed in all the fractions.View full abstractDownload PDF (1127K) -
II. Comparison between the Gains of Spring and Autumn Born Young Steers from Pasture AloneKenroku HAYASHI, Minoru OTA, Takeshi IZAWA, Zenkichi TERUYA, Saburo TA ...1967 Volume 38 Issue 8 Pages 345-350
Published: August 25, 1967
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESS1. Twenty heads of both autumn (A) and spring (S) born Japanese black steers (7.8±0.3 and 13.0±0.4 months old respectively at the start of grazing) were tested on the gains from both native and improved pastures alone.
2. During the whole grazing period of 19/May-20/Oct. the gains of A were 101.8±12.1kg (0.661kg daily) on improved pasture and 84.8±6.1kg (0.551kg daily) on native pasture, and the gains of S were 88.4±18.6kg (0.574kg daily) and 57.0±7.4kg (0.370kg daily) respectively. So the gains of A were higher and they varied less than the gains of S on both pastures.
And the gains from improved pasture were higher and they varied less than the gains from native pasture with A and S.
3. The difference between the gains of A and S was bigger on native than improved pasture, and the difference between the gains from both pastures was shown more remarkably by S than A.
As to seasonal changes of the difference between the gains of A and S, the remarkable difference was observed only during the middle grazing period on improved pasture, whereas during the whole grazing period on native pasture.
From analysing these results, it was presumable that unfavourable enviromental conditions, especialy pasture conditions affecting low nutritional level for animals, forced worse inflence upon S than A.View full abstractDownload PDF (432K) -
Young Sang LEE, Daisaku KUBOTA, Hiroshi MORIMOTO1967 Volume 38 Issue 8 Pages 351-357
Published: August 25, 1967
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSThese experiments were planned to investigate the calcium metabolism and requirement of calcium of laying hens.
The basal diet used in these experiments were about the same diet that were used in the previous experiments, reported on this journal. All experimental diets contained 0.70% of phosphorus, and the calcium contents were 1.46%, 3.09% and 4.5%, respectively.
The experimental methods used in these experiments were exactly the same that were used in the previous experiments.
As a result of these experiments, it was made clear that the balance of the closest to the ±0 line was 3.09% in calcium. From these facts, it was found that the proper requirement of calcium for caged White Leghorn hens was about 3.00 per cent.
Through the results of the above experiments and the previous experiments, it was found that the proper requirements of the phosphorus and calcinm for caged White Leghorn hens, when their rate of egg production was normal, were about 0.70% and 3.00%, respectively.View full abstractDownload PDF (417K) -
VII. On the Operation for Cecum Fistula in Rabbit, and Excretion of Orally Administrated Cr2O3 to Hard and Soft FecesTsutomu YOSHIDA, Makoto KANDATSU1967 Volume 38 Issue 8 Pages 358-363
Published: August 25, 1967
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSTwo kinds of operation to make fistula into cecum of rabbit were established and described fully in the report.
Test diets containing 1%. Cr2O3 were given 150g per day to the operated animals, and Cr2O3 excreted in hard feces (h. f.) and soft feces (s. f.), occasionally that in the cecal contents, were determined. The results obtained are summarized as follows.
1) In the h. f. more Cr2O3 was contained than in the s. f., both of which were collected for 24 hours. Same results were also shown in the both feces collected at an interval of 8 or 2 hours in a day.
2) In regard to the Cr2O3 content of the h. f. which was much different from the s. f., it was suggested that the different extent of absorption in the large intestine might be responsible for the formation of the h. f. or s. f..
3) Less percentages of Cr2O3 in the cecal contents were shown at night.
4) There were the differences of fecal Cr2O3 content between the feces collected at the various times.View full abstractDownload PDF (1667K)
- |<
- <
- 1
- >
- >|