Effects of γ-irradiation on lactating rats and her suckling youngs were studied. Lactating mothers were exposed to γ-ray but suckling youngs were not. Mothers and litters were weighed almost every day to their weaning.
Suckling youngs' body weights increased at normal rate for 2 weeks' experiments after 200r irradiation. In 400r or more irradiation, the litter's weight gain were depressed. In 800r irradiation, the litter's weight gains showed a decrease of 140% against the control group on the tenth day.
However, mothers' body weights decreased with mere 200r irradiation. Though with 400r or more the weights decreased, it had seen some increase period in the first week. In the case of 800r, their weights decreased rapidly and all of them died in 10 days.
In all cases, the mother's death was followed by her suckling young's death.
These results suggested that the effect of γ-irradiation on animals included two independent effects. These were the effect on mother's growth and that on lactation. In this experiment, the effect on a mother's growth was stronger than that on her mammary gland.
Whole-body irradiation of 800r on lactating mothers could not stop their lactation completely till the day of mothers' deaths.

View full abstract

The major objective of this investigation was to study the effect of Morea on the stability of abnormal milk in relation to the salt balance, especially in the relation of the behaviors of calcium and phosphorus to calcium phosphocaseinate.
All the milks, which exhibit normal acidity but coagulate by alcohol test even in fresh condition soon after milking, are generally called "the normal acidity and alcohol-test positive milk". In the present paper, this type of milk is expressed briefly in "abnormal milk".
As a result of this experiment, it was found that the supply of Morea as a medical ration had no effect on the cows producing abnormal milk in the later lactation period, viz., the sensitivity of milk to alcohol test increased by the feeding of Morea. In this case, the Salt Balance of Abnormal Milk and Morea (I) amount of calcium or phosphorus dissolving in milk and that of calcium or phosphorus binding to casein were fairly far from their normal values. But, some favorable effect of Morea as a medical ration on the curing of cows giving abnormal milk in the early lactation period might be expected, not only from the increase of % casein N in total N (casein number) in milk but also from the approach of the salt balance, i. e., the ratio between calcium and phosphorus, with special referench to calcium phosphocaseinate, toward normal value.
The average values for normal milk found in the present experiment are given as fol- lows: total protein 3.00g, casein 2.31g, casein in total protein 77 per cent; total Ca 118.1 mg, ultrafiltrable Ca 35.9mg (30.7 per cent of total Ca), total P 91.2mg, ultrafiltrable P 31.4mg (34.6 per cent of total P) per 100ml; Ca: P 1.33, Bound-Ca: P1. 42 bound-Ca 35.6mg, bound-P 26.0mg per g casein.

View full abstract

1) The sialic acid content in casein measured with SVENNERHOLM'S resin method gave slightly higher value than WARREN'S thiobarbituric acid method.
2) SVENNERHOLM'S resin method was preferably convenient to determine the sialic acid in skim milk, and the sialic acid content in whole casein was measured easily with WARREN'S thiobarbituric acid method.
3) The sialic acid content in individual milks varied from 0.40 to 0.59% and that in colostrum immidiately after calving showed remarkably high values.

View full abstract

In order to ascertain the influence of inclination of tube upon erythrocyte sedimentation rate (ESR for short) in domestic animals, the variation rate with the change of tube inclina tion were calculated at the range of 0°-5°slope to vertical;
Slope variation rate=ESR at 5° slope to vertical-ESR at the vertical position/5 (unit in mm)
The results obtained are shown in Fig. 1. The slope variation rate increased to maximum value and then decreased. The correlation between the erythrocyte sedimentation types and the slope variation rate was shown in Fig. 2. The appearance of the maximum point delayed by the sedimentation types moved from acceleration types to retardation types (Table 1). The change of the slope variation rate seemed to be in close connection with the erythrocyte sedimentation types.
Moreover, the variation ratio with the change of the tube inclination were calculated;
Slope variation ratio=Slope variation rate/Average ESR. at the vertical position×100(%)
The results obtained are shown in Table 2 and Fig. 3.
The influence of the tube inclination upon the erythrocyte sedimentation in domestic animals were so great that the sedimentation rate should be measured at the exact vertical position.

View full abstract