Ovotransferrin (OTf) was purified from different species of birds by two different methods; one using CM- and DEAE-Sephadex column chromatography, and the other using immuno-adsorbent column chromatography. By both methods, OTf was purified as a single protein from albumen of chicken, quail, duck, and pigeon. The molecular weight of OTf of chicken, quail, duck, and pigeon are 77500, 75100, 76400, and 77500, respectively when measured by sodiumdodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE). A double immuno-diffusion test showed that precipitin lines of purified OTf and transferrin (Tf) of each bird fused together on an agar gel with chicken OTf antiserum, while the lines between different birds showed a spur pattern. This result shows high similarity of OTf and Tf within a species. Single radial immunodiffusion tests and immunoadsorbent column chromatography showed the content of OTf in albumen of chicken, quail, duck, and pigeon to be 12.37±0.20%, 11.20±0.60%, 1.61±0.63%, and 13.04±1.30%, respectively. These OTfs and Tfs, purified by using an immuno-adsorbent column, were shown to be a single polypeptide on SDS-PAGE, suggesting that both OTf and Tf do not show the moleculer weight heterogeneity observed in bovine Tf.
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