In the previous paper, it was reported that the cardiac hypertrophy was produced as a parmanent effect of high altitude exposure in mice. The forced exercising test was carried out on mice at sea level produced the cardiac hypertrophy resembling to that mentioned above. Fifty mice of ICR-JCL strain born at sea level were used and randomly divided into four groups; ten for control, and fifteen for test groups in each sex. For 50 days from the 3rd to the 10th week after birth, the exercising groups were loaded daily by the treadmill for mice under the time schedule in which total distance of running was 37, 630m. At the 11th week after birth, electrocardiograms of these mice were recorded and the organ weights and the thickness of ventricular walls were measured. Difference between the control and theexercisinggroupswastestedstatistically(α=0.05)ineachitem.
The results obtained are as follows: 1) The kidney(in both sexes), the spleen(in male), the liver(in both sexes), the brain(in female) and the heart(in female) weights per body weights(0/00) of the exercising group were significantly greater than those of the control group. 2) No significant differneces were recognized in adrenal weights of both groups in both sexes, although adrenal weight increased in mice exposed in high altitude remarkably. 3) The right ventricular walls of the exercising group increased in thickness and the ratio of the right to the left markedly decreased in both sexes. 4) The durations of QRS complex prolonged significantly in the exercising group of both sexes, but no circulatory disorders were found in all individuals. 5) These results demonstrated that the cardiac hypertrophy without dysfunciton was produced by the high altitude exposure and/or by the forced exercise in mice, as a common responce to these conditionings.

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The contents of Na and K in pork from 65 pigs of accurately known history were determined by flame hotometry and the differences due to various factors(year, season, breed, muscle and sex) were analyzed statistically. The data on Na and K content of pork wereclassified into five groups by the factors and summarized in Tables 1, 2, 8 and 9. The(overall) means of 219 samples were as follows: Na, 63.0±1.3mg% and K, 326±4mg%(wetbasis); Na, 58.0±1.5mg% and K, 298±3mg%(ash basis). There were marked differences in Na and K content between muscles, and the muscle differences in Na content were largerthan those in K content. The differences between years and between breeds were significant, while those between seasons and between sexs were scarcely significant.

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Up to the present, the susceptibility to estrogen of immature chicken has been expressed by the serum vitellin reaction regression equation(Y=bX+k) obtained from the degree of the serum vitellin production during 10 days after the beginning of estrogen administration by the author et al. In this case, the serum vitellin production was detected by the use of immunological precipitin test. However, the above mentioned immunological method was laborious and time consuming. Accordingly, the author considered that the more simplified and rapid method of measuring the serum vitellin concentration must be adopted. Two tenth ml of blood was collected from the wing vein into the1ml injection-syringe filled previously with 0.8ml of 0.85% physiological saline solution(containing 2% sodium citrate in order to prevent blood coagulation). Blood floating solution collected from the estrogen-administered chicken daily was centrifuged and the supernatant solution was used for observation of serum vitellin reaction and also of turbidity. For the measurement of turbidity, a certain amount of acetone was added into a part of the supernatant solution and proteinic substance in blood plasma was precipitated. The supernatant fluid was discarded after centrifugation and the precipitate was homogenized with a certain amount of distilled water. Then, the turbidity of the fluid mentioned above was measured with a turbidimeter and the correlation between the serum vitellin reaction titers and the turbidity was estimated. Simultaneously, the amounts of acetone to be added to blood plasma solution, the hours of immersion in acetone and suitable temperatures were examined. On the other hand, the chickens were sacrificed after 10 days from the beginning of estrogen administration and the blood serum collected from the chickens were ultracentrifuged (40, 000 r. p. m., 105, 400×g, 4hrs). Each layer (three layers) of the blood serum seperated by ultracentrifugation was used for observation of serum vitellin reaction and turbidity.1. The best method for measurement of turbidity was as follows: One-half ml acetone was added to 0.2ml of blood plasma solution. The mixture was incubated at 25°C for 2hrs. After the incubation, the mixture was centrifuged and the supernatant fluid was discarded. Five ml of distilled water was added to the precipitate and homogenized. Then, the turbidity was measured. 2. The correlation coefficients between the vitellin reaction titers and the turbidity came up to 0.832(male), 0.892(female), after 3 or 5 days from the beginning of estrogen administration, respectively. 3. The correlation coefficients between the vitellin reaction regression coefficient and the regression coefficient of the turbidity on the duration of estrogen administration were 0.526(summer), 0.807(autumn) in female and 0.708(summer), 0.753(autumn) in male. 4. Three layers(top and middle layers and the precipitate) obtained by the ultracentrifugation of the estrogenized chicken serum were se-perated visually with a pipette. The correlation coefficients between the serum vitellin re-action titers and the turbidity of each layer were 0.937(top layer), 0.466(middle layer) and 0.043(precipitate) respectively. From above results, it appeared that the turbidity method by use of acetone would be applicable in place of the conventional serum vitellin reaction method by precipitin test.

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For good preservation of pickled light skin, more detailed investigations are necessary not only on the pickling condition but also on the selection of raw material and the beamhouse operations. Calf skins were left standing in the green state for the different hours and applied with or without salt curing for 40 days. They were then processed into pickled skins and followed by one or three months storage. From solubilized nitrogen content of the pickled skins after storage and properties of the chrome upper leather prepared from them, it can be said that pickled skin may be prepared well from both the green and the salted stock if it is quite fresh, but when not so fresh, it should be prepared directly from the green stock. When solubilized nitrogen content of the pickled skins after storage, which have been prepared under the different conditions of reliming and pickling, as well as the properties of resultant chrome leather are both taken into consideration, the reliming should be stopped within one or two days and the pickling should be done using more than 12% sodium chloride and 1.0-1.4% sulfuric acid, if the shelf time of pickled skins is three months.

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The relationship between respiration and glycolysis, and motility of goat spermatozoa were studied under aerobic condition, using fluoride, mono-iodoacetate, 2, 4-dinitrophenol, arsenite, cyanide and fluoroacetate as metabolic inhibitor. The results obtained are as follows. 1. Fluoride and mono-iodoacetate inhibited the aerobic glycolysis, oxygen uptake and motility of spermatozoa in the presence of fructose. 2. 10-4 and 10-3M cyanide inhibited the oxygen uptake while the lactic acid production was stimulated greatly, as followed by good motility of spermatozoa. Similar com-pensatory glycolysis was observed by the addition of arsenite and 2, 4-dinitrophenol, though the initial vigorous motility was not supported. Fluoroacetate inhibited res-piration and motility of spermatozoa, but it was of no effect on aerobic glycolysis. 3. 2, 4-dinitrophenol appeared to inhibit the oxidative phosphorylation of spermatozoa

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The effect of chlorpromazine hydrochloride(CPH) on survival and metabolism of goat and bull spermatozoa was examined. The results obtained are as follows. 1. The addition of 10 to 40mg per cent of CPH was beneficial to the survival of spermatozoa, particularly of bull spermatozoa, which stored with egg-yolk citrate buffer at 4°C. 2. CPE stimulated the oxidation of pyruvate, acetate and fructose by spermatozoa at lower levels while it inhibited the oxidative activity and motility of spermatozoa at the 10mg level. 3. CPH inhibited the anaerobic glycolysis of spermatozoa when it was added to the sperm suspension with glucose before the preliminary incubation period.

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