Laying hens were fed the diets supplemented with 10% of fat or oil (tallow, lard, soybean oil or cocount oil) for a period of 50 days, and the changes of fatty acid compositions of yolk lipids and the fatty acid distribution in yolk triglyceride affected by dietary fat and oil were studied. The feeding of diet supplemented with tallow or lard resulted the increase in C
18:1 acid, the decrease in C
16:0 and C
18:2 acids in total lipids of egg yolk and the decrease in C
20:4 and C
22:5 acids in phospholipid of eggs. The feeding of soybean oil resulted the decrease in C16:0 and C
18:1 acids and considerable increase in C
18:2 acid in egg yolk lipids, and in phospholipid fraction of eggs, C
22:5 decreased and C18:0 decreased on soybean oil feeding. The feeding of coconut oil resulted the increase in C14:0 and C16:1 acid and the decrease in C
18:1 and C
18:2 acids in total and neutral lipids of egg yolks, and on the other side, resulted the increased in C
18:1 acid and the decrease in C
20:4 and C
22:5 acids in phospholipid of eggs. The fatty acid distribution in triglyceride was determined with the aid of pancreatic lipase hydrolysis. Although the fatty acid distribution in triglyceride of tallow was remarkably different from that of lard, not so much difference was observed between the yolk lipid from hens fed tallow and from hens fed lard on the fatty acid distribution in their triglycerides. The yolk lipid from hens fed soybean oil was considerably different with that from hens fed coconut oil on the fatty acid compositions of triglycerides, but in any case, C
16:0 and C
18:0 acids were found to be preferentially esterified at the 1, 3-position in triglycerides and C
18:2 acid predominantly linked at the 2-positions. The triglycerides in yolk lipids such as SUU and SUS tended to decrease and SSS, SSU and UUU increased on tallow feeding to hens. On lard feeding, SUS decreased and UUU increased in yolk lipids. The feeding of soybean oil resulted the considerable decrease in SUS and the increase in UUU in yolk lipids, and on the contrary, coconut oil feeding resulted the increase of SSS, SSU SUS and USU, and the decrease of SUU and UUU in yolk lipids. Concerning to the major component triglycerides in yolk lipids, PLP, PLO and OLO decreased and POO and OOO increased on tallow feeding to hens. On lard feeding, PLP and POPt decreased and OOO increased in egg lipids. The soybean oil feeding to hens resulted the decrease of POP, POSt POO and OOO in yolk lipids, and the distinct increase of PLP, POPt, StLO, PLL, OLO and OLL. On the other hand, coconut oil feeding resulted the decrease of PLP, POO, PLO, OOO and OLO, and the increase of MyOP and MyOO in yolk lipids. (Abbreviation used in this were as follow; S: saturated, U: unsaturated, P: palmitic, Pt: palmitoleic, St: stearic, O: oleic and L: linoleic acid).
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