Rabbits were fed on diets containing 1.0% chromic oxide. They received a single injection into the marginal auricular vein of an isotonic solution containing radioactive phosphate and slaughtered 5 or 20 hours after injection. Chromic oxide, radioactive phosphorus and total phosphorus were determined in the contents of various portions of the gastro- intestinal tract.
The results obtained are summarized as follows.
1) The chromic oxide content of dry matter was a little higher in the stomach and fairly lower in the small intestine than in the feed, and rose gradually down to the anus.
2) The radiophosphorus content of dry matter was very low in the stomach, but very high in the small intestine and lowered remarkably down to the anus.
3) The total phosphorus content of dry matter was very low in the stomach, but fairly high in the small intestine and in the cecum, and lowered gradually down to the anus.
4) Remarkable absorption was thus confirmed in the large intestine, especially in the colon and rectum, in the rabbit

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1. Relations between the fluorine contents of the animal body, drinking water, and feedstuffs, and chronic fluorine poisoning were studied by determining the fluorine contents in them.
2. It was found that fluorine contents were higher in bones than in teeth, and in cattle than in horses.
3. The fluorine contents of teeth, bones, and hairs of cattle raised in hi h-fluorine areas were 2 to 10 times, 2 to 10 times, and 2 to 5 times, respectively, as high as those in low-fluorine areas. Thsre was no difference in fluorine content among the blood, liver, muscles, and milk.
4. In high-fluorine areas, such as the Aso volcano region, the fluorine contents of bones or teeth of domestic animals with mottled teeth were 2 to 5 times as high as those of domestic animals without mottled teeth.
5. The fluorine content of drinking water in high-fluorine areas was 5 to 40 times as high as that in low-fluorine areas, but there was no difference in fluorine content with regard to feedstuffs.
6. When fluorine was administered to rats and rabbits, the fluorine contents of bones and teeth were 5 to 8 times, and those of hairs, blood, heart, spleen, brain, and muscles were about 2 times as high as those in normal animals. Thre was no difference, however, in fluorine content with regard to the liver, kidney, and lung.

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Italian ryegrass was cut on Aprill 30 (I), May 10 (II), May 19 (III, before heading), and May 24 (IV, heads just showing), and four lots of sun-dried hay were made. They were analyzed and evaluated for digestibility in rabbits. The results obtained are summarized as follows.
1. The later was cut the grass, the poorer was the hay made of it in crude protein and crude fat and richer in crude fiber. Differences in compsition between hay III and hay IV were remarkable.
2. The later was cut the grass, the lower was the digestibility of all the nutrients of the hay made of it. The digestibility of hay IV decreased remarkably.
3. The later was cut the grass, the poorer was the hay made of it in digestible crude protein and total digestible nutrients. The contents of digestible crude protein and total digestible nutrients of hay IV lowered remarkably.
4. Yields per are of dry matter, digestible crude protein, and total digestible nutrients were calculated, and the optimum stage of maturity to cut the grass was discussed.

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In order to investigate the change in the quality of proteins of grass during growth, nitrogen distribution in lorcharj grass (Dactylis glomerata) was observed. The leaf, stem and head at various stages of growth, were extracted successively with water, 10% NaCI, 70% ethanol, 0.3% NaOH, and hot 0.3% -alkaline 60%-ethanol. Then total and protein nitrogen in the extracted fractions were derermined.
Results obtained are, as follows
1) In each part of the plant, total nitrogen was distributed mostly in both the hot-alkalineethanol-soluble and the water-soluble fractions, and the ratio, of total water-soluble nitrogen decreased as the plant grew.
At the later stage of growth, the ratio of NaOH-soluble and insoluble nitrogen in the leaf increased. The insoluble nitrogen in the stem and ethanol-soluble and NaOH-soluble nitrogen in the head also increased at the later stage (table 2).
2) The distribution of protein nitrogen changed in the same way as that of total nitrogen. However, the degree of change during growth was higher in protein nitrogen than in total, nitrogen (table 3).
3) As expressed with the percentage for dry matter of the sample, water-soluble and hotalkaline-ethanol-soluble protein nitrogen decreased rapidly as the grass grew. In the other fractions protein nitrogen was generally very small in quantity and its level hardly changed. Only in the head of the grass at the mature stage, however, the level of ethanol-soluble and NaOH-soluble protein nitrogen was considerably high, showing the character of proteins of the grain (fig. 4).
4) Non-protein nitrogen (NPN) was distributed mostly in the water-soluble fraction, but. a considerable amount of NPN was found in the hot-alkaline-ethanol-soluble and NaCI-soluble fractions.
In the leaf, the ratio of water-soluble NPN increased as the plant became mature. On the contrary, hot-alkaline-ethanol-soluble NPN decreased (table 4).
5) When the nitrogen content of each extracted fraction was calculated as percentage for dry matter of whole plant, taking account of the ratio of the leaf, stem, and head at various stages of growth, a rapid linear decrease of water-soluble protein nitrogen during growth was, found very clearly. Hot-alkaline-ethanol-soluble protein nitrogen also decreased distinctly (fig. 6).

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Nitrogen distribution and its change were studied with the aftermath of orchard grass (Dactylis glomerata). The grass which grew after cutting on May 15 (I), May 15 and July 5 (II), and July 5 and September 19 (III), respectively, was analyzed in the course of growth, by using the method described in the previous report.
Results obtained are as follows :
1) In all samples, more than half of the total nitrogen or protein nitrogen was extracted with hot alkaline ethanol. The ratio of water-soluble nitrogen was rather low in (I) and (II), and its level lowered as the grass grew. The ratio of water-soluble nitrogen was higher in (III) than in (I) and (II), but lower than in the grass of first-cutting in spring.
The ratio of insoluble nitrogen was high in (I) and (II), especially in the later stage of growth, more than 20% of protein nitrogen being found in the grass (table 2).
2) The content of hot-alkaline-ethanol-soluble protein nitrogen was as highas 1∼2% of dry matter of the sample. The water-soluble protein nitrogen content was much lower in (I) and (II) than in (III). The insoluble nitrogen content was considerably high in (I) and (II), and the protein nitrogen extracted with NaCI, ethanol, or NaOH was very low in percentage of dried material (fig 2).
3) Non-protein nitrogen was found much in the hot-alkaline-ethanol-soluble fraction, which was followed by the water-soluble and NaCI-soluble fractions (fig. 3): 4) Judging from the data above and the results described in the previous paper, remarkable differences in nitrogen distribution and the content of protein nitrogen or non-protein nitrogen in each fraction were found between the first-cut grass and the aftermath. Also between the grass which grew after cutting in summer and that in autumn, an evident difference in nitrogen distribution was recognized.

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1. This is a supplementary study to the previous work on the behaviors of added calcium and released phosphorus, and includes a time-course study on non-protein nitrogen (NPN) produced from.κ-casein by proteolysis with pure rennin.
2. To make clear the behaviors of ion-exchangeable and non-ion-exchangeable calcium in the soluble part produced from the casein solution, the authors worked out for a hypothetical explanation.
3. The produced amounts of phosphorus and NPN were variable according to the methcd. of precipitating and filtering para-(κ)-casein. Such difference affects the components of the dialysate which remain.
4. The proteolysates produced from κ-casein by the action of rennin in the presence of calcium were different both quantitatively and qualitatively from the products produced with the absence of calcium. The amount of calcium added to the substrate solution under the rennin. activity affected the results of dialysis.
5. A spot of rennin with calcium was found on paper-chromatogram. When there was no calcium near this spot and a spot (Rf) of aspartic acid, the former spot was not detected with ninhydrin.

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