The present investigation was performed to examine wether the polygraphic monitoring and recording method developed by SUGANO et al (1962) for mouse was applicable or not in determining the normal values in several physiological factors, i. e. body temperature, heart beat, respiratory rate, blood pressure and sedimentation rate of erythrocyte in chick. The summarized results were as follows:
(1) The experiments were made by using white leghorn chicks belonging to the same strain. The age of the chicks distributed from 1 to 10 weeks old after hatch. Growth of the chicks used was recognized as normal, with reference to weekly increase of body weight. The sexual difference was found in the chicks older than 6 weeks.
(2) The body temperature of 1 week old chicks was about 40.8°C in males and was about 40.6 in females, and increased gradually untill it reached at about 41.3°C in the both sexes of 10 weeks old chick, passing through the maximum value of 41.7°C in 6 weeks old chicks.
(3) The heart beat rate continued to increase to reach its maximum of 470 in 2 to 4 weeks old chicks and decreased to about 370 in the 10th week of age in which the sexual dif-ference in the rate was detected, females showing higher values.
(4) The respiratory rate showed a remarkably wide range of variation, but it presented a gradual decrease accompanying with growth until it dropped to about 40 in 10 weeks old chicks.
(5) An increase of the blood pressure was presented during the initial six weeks of growth and it reached to its maximum value of 140mmHg in the 10th week.
(6) The sedimentation rate of erythrocyte was 2.0 to 2.5mm per 30 minutes at the age of 6 to 10 weeks after hatch.

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Tne purpose of the present experiment was to examine the changes of physiological values possibly produced by the medication of a few of agricultural chemicals such as Diethyl Pnitrophenyl monothiophosphate (Parathion) and 1, 2 dicarboethoxyethyl dithiophosphate (Marathion) and make a detailed analysis of the changes from the view point of polygraphy in growing chicks. The results obtained were as follows:
(1) Principal symptoms appeared in Parathion and/or Marathion poisoning in chicks presented a similarity to those observed in adult fowls and mammals medicated with them. Urination, lacrimation, salivation, piloerection and defecation etc. were usualy detected and muscular tremor was also frequently recognized. The vigorous difficulty of breathing and convulsion produced by injection of a large quantity of the drugs were always followed by death. The toxic potency of Parathion was presumed to be about 10 times stronger than that of Marathion.
(2) The injection of Parathion into the pectoral muscle produced a prompt decrease of the heart beat rate and respiration frequency and a sudden decline of blood pressure. In this case, the death occured usually in few minutes after medication, despite quantity of the chemicals injected being distributed over a wide range of 15 mg/kg to 60 mg/kg.
(3) The drug effect was influenced by density of the solution injected. An adequate dose for observing the typical phenomena in phosphate poisoning was 720 mg/kg in Marathion and 60 mg/kg in Parathion, by which the chicks could be kept alive long enough to the observation time of about 60 minutes.
(4) The changes in physiological measures in case of the poisoning by organic phosphate medication followed after the above-mentioned clinical or external phenomena. A prompt decrease of the heart beat and a rapid decline of the blood pressure appeared almost simultaneously. An acceleration of respiratory movement gave rise immediately to occure before the death and its abrupt cease accompanying with vigorous convulsion.
(5) It seems that Parathion acts further stronger to 4 weeks old chicks than 6 or 8 weeks old chicks from the vieow point of length of their living time.

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The phospholipids in milk were analyzed 5 times by ROESE-GOTTLIEB method (no sodium chloride addition), ROESE-GOTTLIEB method (sodium chloride addition), methyl alcohol-chloroform direct extract method, TCA precipitation BLOOR reagent extract method, acid precipitation BLOOR reagent extract method and ammonium sulfate salting out BLOOR reagent extract method, and the results were studied by Tukeys multiplex examination method.
There were no significance among ROESE-GOTTLIEB, method (no sodium chloride addition), ROESE-GOTTLIEB method (sodium chloride addition), methyl alcohol-chloroform direct extract method and acid precipitaticn BLOOR reagent extract method which the authors devised, but between TCA precipitation BLOOR reagent extract method and other five methods there were significances with the significant level of below 1%, and also there was significances between ROESE-GOTTLIEB method (no sodium chloride addition) and ammonium sulfate salting aut BLOOR reagent extract method with the significant level of below 5%.

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In our previous reports, it was demonstrated that the procedure consisting of extraction by kaolin and purification by column chromatography on tricalcium phosphate was effective for detection and estimation of gonadotropic activity in the urine of goat. However, it was found that the technique of column chromatography was laborious and time-consuming. In the present report, a simplified procedure for purification of the kaolin extracts obtained from the urines of female goats was described.
The kaolin extracts (crude UGS) from the urines of dry-estrous cyclic, pregnant, lactating goats and postmenopausal woman were dissolved in 0.002 Mdisodium hydrogen phosphate and purified further by batchwise adsorption of inert proteins onto Ca3 (PO4) 2. The supernatants [unadsorbed fraction on Ca3 (PO4) 2] were collected, adjusted to pH 5.5 and filtrated. The precipitates by addition of cold acetone to the filtrates were designated as UG. In addition, materials which had been adsorbed to the Ca3 (PO4) 2 were eluted with 0.02 M-trisodium phosphate. These eluates were adjusted to pH 5.5 and centrifuged. The supernatants were added cold acetone and the precipitates were dried. The final products were designated as UG'.
Shince the solutions of UG obtained from the goats' and human urines were clear and non-coloring, the sugar contents of these solutions were measured by the method described previously. However, the sugar levels in UG, could not be measured because of its ten or brown color. Total gonadotropic activities of UG and UG' were assayed biologicall by the uterine test in immature mice.
In the biological assay of UG preparations obtained from the urines, significant increases of uterine weights of the assay animals were found at the dose levels of 16 to 24 hour-equivalent of urine in cyclic, 24 hour-equivalent in pregnant and 32 to 48 hour-equivalent in lactating goats. The sugar values of UG fractions of cyclic and lactating goats measured by the chemical assay were related closely to the biological activities. Gonadotropic activities of UG' from the goat's urine could not be detecsed until the dose level of 48 hour-equivalent of urine.
It was concluded that the simple method for purification of the kaolin extracts reported in the present study was suitable to prepare the urinary gonadotropin concentrates from female goats in various reproductive states.

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A theoretical study was made on the loss of accuracy of the progeny test of a dairy bull when a part of sample daughters happen to have actually been sired by bulls other than the one supposed to have sired the sample.
The following formula was derived for the accuracy (correlation between the mean phenotype of the sample daughters and the breeding value of the bull concerned) in such a case of misidentification of parentage:√(1-k)2h2n/4+(n'-1)h2+4(n-1)c2, where n'=n{(1-k)2+k2/n}.
Here, besides that h2 stands for the heritability, n for the size of sample and c2 for the part of the phenotypic correlation between sample daughters that is due to any causes other than genetic influence of the sire (s), k represents the portion of the sample that has been got of sires different from the one under test and m the number of these actual sires.
A few numerical examples were given of accuracy of progeny test for a variety of combina-tions of selected values of all the factors appearing in the formula, i. e. k, m, h2, n and c2.
General conclusion, so far as the heritability is between 0.1 and 0.3 and the size of sample daughter group between 20 and 30 as is often the case in progeny testing of dairy bulls, is that the average losses of accuracy of progeny test due to misidentification of sire-daughter relationship are roughly 5% and 10% for k=0.1 and k=0.2, respectively, the accuracy expected when k=0 and also c2=0 being set at 100%.

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