The objective of this study was to investigate the seasonal incidences of yeast and the seasonal changes of yeast counts (cfu/m
l) in a high quality raw milk produced by healthy Holstein cows (≤1×10
5 somatic cells/m
l) bred in Obihiro University of Agriculture and Veterinary Medicine and in three local dairy farms near the University campus. The populations of yeasts in teat milk, bulk milk and lorry tank milk as well as in one of teat surfaces which were collected in each month from July, 1999 to June, 2000 were counted. The incidences of yeasts in teat milk, bulk milk, lorry tank milk, and in one of teat surfaces were 47.9%, 100%, 100% and 70.1%, respectively, whereas the mean of yeast counts in these samples were 1.8cfu/m
l, 28cfu/m
l, 66cfu/m
l and 52cfu/cm
2, respectively. The levels of yeast counts, which occupied over 90% of the cumulative frequency distributions of the incidence, were ≤10cfu/m
l (94.4%) in teat milk, ≤100cfu/m
l (97.8%) in bulk milk, ≤150cfu/m
l (91.7%) in lorry tank milk, and ≤150cfu/m
l (90.8%) in teat surfaces. It was recognized that the yeast counts in these raw milk samples and in teat surfaces overall increased from summer to autumn, and then decreased from winter to spring. It was found that the four major pathogenic yeast species concerning mastitis (
C. parapsilosis, C. lambica, C. catenulata, C. rugosa) in teat milk of healthy Holstein cows were detected in summer, not in winter.
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