The adipose tissue fats from different dept sites of swine with soft fat and normal fat were investigated by stereospecific analysis and argentation thin-layer chromatography procedure to reveal some features of soft fat pork lipids. Fats of swine with soft fat contained more C
18:2, C
18:3 and total unsaturated fatty acids and less C
16:0 and C
18:0 acids than those of normal fat. The adipose tissue lipids, both of soft fat and normal fat, had similar fatty acid distributions in triacylglycerols with most of the C
14:0, C
16:0 and C
16:1 acids in position 2 and much of the C
18:0 acid in position 1, although kidney leaf fat of swine with normal fat with high C
18:0 acid content had significant amount of this acid in position 3. On the other hand, C18:1 and C
18:2 acids preferentially esterified to positions 1 and 3. The lower C
16:0 acid concentration of soft fat than that of normal fat were mainly accounted for the difference of this acid content in position 1. And the large amounts of C
18:2 acid in soft fat were resulted from the higher concentrations of this acid in all three positions of soft fat triacylglycerols than in those of normal fat. Back fats of soft fat pork had less concentrations of C
18:0 acid in psition 3 than those of normal fat in corresponding of position, and it seemed that these decreased amounts of C
18:1 acid in position 3 of soft fat triacylglycerols were largely compensated by the increased amounts of C
18:2 acid in the same position. The amounts of component triacylglycerols of adipose tissue fats were calculated with results from the known fatty acid distribution in the intact triacylglycerols, assuming a 1-random, 2-random, 3-random arrangement. Fats of swine with soft fat contained more S
2U and U
3 triacylglycerols and less S
3 and S
2U than those of the corresponding depot site fats of swine with normal fat. The triacylglycerols of adipose tissue fats were separated according to degree of unsaturation by argentation thin-layer chromatography. The proportions of the more saturated triacylglycerol species such as S
3 and S
2M were significantly less and the proportions of the more unsaturated species with three or more double bonds such as SMD, SD
2 and M
2D were greater in soft fat pork lipids than in normal fat. (Abbreviations used in this were as follows; S: saturated, U: unsaturated, M: monoenoic, and D: dienoic fatty acid)
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