日本農芸化学会誌
Online ISSN : 1883-6844
Print ISSN : 0002-1407
ISSN-L : 0002-1407
23 巻, 6 号
選択された号の論文の14件中1~14を表示しています
  • 田邊 脩, 中村 忠夫, 都留 春夫, 内田 哲生, 山口 米子
    1950 年 23 巻 6 号 p. 229-230
    発行日: 1950/01/01
    公開日: 2008/11/21
    ジャーナル フリー
    I. We selected Aspergillus oryzae suitable for Amylo-process.
    Results obtained It is decided that 3 strains (Asp. orzae HAYAKAWA, Asp. oryzae A, Asp. Sake E.) are the most powerfull for liquefaction and saccharification of starch, and the most suitable for Amylo-process.
  • 田邊 脩, 都留 春夫
    1950 年 23 巻 6 号 p. 230
    発行日: 1950/01/01
    公開日: 2008/11/21
    ジャーナル フリー
    II. We selected Black Aspergillus suitable for Amyloprocess.
    Results obtained: It is decided that 2 strains (Black Asp. R-15-0635 and R-7-0436) are the most powerfull for liquefaction and saccharification of starch.
  • 芦田 淳, 中村 定市, 遍々 古孝子
    1950 年 23 巻 6 号 p. 231-237
    発行日: 1950/01/01
    公開日: 2008/11/21
    ジャーナル フリー
    d-Glucose in alkaline solution is in equilibrium as shown in formula (1).
    If there is an acceptor of the hydrogen anion formed in this equilibrium, the reaction will proceed to the right and produce d-gluconic acid. As the acceptor one of the following was used ketones (cyclohexanone or acetone), double bond-containing compounds (cinnamic acid or maleic acid), nitrobenzene, or atonial oxygen (air). d-Glucose and the equivalent amount of one of these acceptors were dissolved in about 2% aqueous solution. of sodium hydroxide After the Raney nickel was suspended, this reaction mixture was shaken at the room temperature.
    In all cases except nitrobenzene d-glucose was oxidised to d-gluconic acid, while these acceptors were reduced. In the case of nitrobenzene d-glucose was further oxidised.
  • 小田 信, 平野 稔
    1950 年 23 巻 6 号 p. 237-239
    発行日: 1950/01/01
    公開日: 2008/11/21
    ジャーナル フリー
    1. プルフリッヒ比色計を用いヒドロオキシラミン法により比色すれば培地(200~600u/mL)中のペニシリン直接定量しうる。所要時間は30分以内,誤差は10%前後である。
    2. D=10mmの場合,培地用係數は3773,ペニシリンGに對しては3790である。
    3. ペニシリナーゼを使用せずともアルカリ處理で充分である。囘收試驗は84~100%である。
  • (第3報)大麻纎維原料のペクチン質を分解する一好氣性細菌と分解せざる一好氣性細菌に關する問題
    中濱 敏雄, 青木 純次
    1950 年 23 巻 6 号 p. 240-244
    発行日: 1950/01/01
    公開日: 2008/11/21
    ジャーナル フリー
    1. 大麻纎維原料の醗酵精練に對して有用なる2種の好氣性細菌を分離し便宜上それぞれ大麻I5菌,及び大麻F菌と假稱した。
    2. 分類學的の特徴を檢索いた處,大麻F菌はBacillus mesentericusの變種と認められたのでB. mesentericus var. AOKIと命名した。
    3. 大麻I5菌は大麻原料中のペクチン質を分解する酵素を生産し之に依り精練作用を有する事を認め得た。B. mesentericus var. AOKIは大麻原料のペクチン質に對し認む可き分解作用を有せざるにかゝわらず大麻原料の浸漬液に移植する事に依り著しく醗酵を促進せしめる事が出來た。之は本菌がペクチン質分解力を有する有用微生物と推定する事が出來る。
  • (第4報)大麻の醗酵作用を有する嫌氣性細菌とその共棲微生物に關する問題
    中濱 敏雄
    1950 年 23 巻 6 号 p. 245-249
    発行日: 1950/01/01
    公開日: 2008/11/21
    ジャーナル フリー
    大麻原料より其の醗酵精練作用を有する2株の嫌氣性細菌を分離し夫々AnB菌及びF14B菌と假稱した。
    AnB菌及びF14B菌に依る大麻原料の純粹醗酵試驗を行ひ之等の嫌気性細菌がペクチン質分解作用を有する事を確めた。
    ペクチン質分解力を有せざる好気性細菌B. mesentericus var. AokiとAnB菌又はF14B菌との共棲の下に大麻原料の醗酵精練は滿足に行はれる。從つて上記好氣性細菌わ接種する事に依り精練作用が促進されるのは本菌が豫め原料に生育する嫌氣性有用細菌と共棲する爲であろうとの前報の推定を確める事が出來る。
    AnB菌及びF14B菌はペクチン分解力を有する好氣性3號菌及び大麻I5菌とも共棲する。
    AnB菌及びF14B菌の分類學的特性を檢索し夫々Clostridium sphenoides及ひせClostridium butyricumの一變種である事を認めた。
    終りに臨み本研究に就て御指導を得たる京大片桐,北原兩先生に對し深謝の意を表する。
  • 第17報補遺
    阿部 又三
    1950 年 23 巻 6 号 p. 250-251
    発行日: 1950/01/01
    公開日: 2008/11/21
    ジャーナル フリー
    In the previous paper(1) the author reported that the quantity of the ergot alkaloid which could be found both in the mycelium and in the culture medium at a certain period of the culturing process was considered to be the balance, in the former, between the total alkaloid permeated into cells and that decomposed therein, while in the latter, between the total alkaloid excreted into the culture medium and that permeated into cells therefrom, by that time; that is, the decrease of the ergot alkaloid in the culture medium culturing the ergot fungus was attributable, not to the decomposition, but to the permeation into cells.
    In the present work the author has dealt with the observation that an ergot alkaloid in certain solutions decreases in the same manner as in the culture medium culturing the ergot fungus when these were subjected to microbial contamination. The study has resulted in the conclusion that the observation should also be ascribed to the permeation of alkaloid into cells.
  • (第1報)家蠶膿病體のプロテアーゼ作用に及ぼす水素イオン濃度及び加温の影響
    吉原 典子
    1950 年 23 巻 6 号 p. 252-254
    発行日: 1950/01/01
    公開日: 2008/11/21
    ジャーナル フリー
    The multiplication of viruses depends upon the denaturation or polymerization of normal protoplasma proteins in living tissues. Therefore, in order to clarify the mechanism of virus formation the metabolism in the cells must be investigated. As it has been generally recognized that the virus molecules are no more than the polymerized nucleoproteins, I have begun to study the changes and properties of proteases in the virus-diseased tissues. At first, the investigation on the nature of proteolytic enzymes in polyhedral-diseased silkworms was performed. The silkworm is the most suitable animal for this purpose, because it grows very rapidly and chemical changes in its body are vigorous. Furtheremore, this insect is easily attacked with a virus disease. The polyhedral-diseased silkworms were treated with acetone and an enzyme praparation was prepared. The proteolytic activity of this preparation is somewhat low in acid side, most weak at neutral reaction and strong at higher pH. The action of the protease is very weak below 10°, strongest at 50° and over 70° the activity is losta in few minutes. At all times it was found that the protease activity of diseased body is considerably higher than the activity of healthy one.
  • (第2報)家蠶に於ける實驗的バイラス發病と蛋白酵素との關係
    吉原 典子
    1950 年 23 巻 6 号 p. 254-257
    発行日: 1950/01/01
    公開日: 2008/11/21
    ジャーナル フリー
    In the previous paper some properties of proteases of virus-diseased silkworm were described. I have assumed that before the formation of virus molecules in living cells protoplasma proteids, especially nucleoprotein must be rearranged. Furthermore it is supposed that the proteolytic enzymes in living tissues participate certainly in this rearrangement of proteins. As it was reported, in the silkworm body the virus can be formed by artificial means. A group of silkwors was warmed, the second group was fed with mulberry leaves which were wetted with a hydroxylamine solution and the third was given barium peroxide. The treated worms were attacked with a polyhedral disease. It was found that the proteolytic action of worm body was elevated by these treatments. The maximal activity of proteases was observed in the virus-diseased worm.
  • 和田 八三久, 新保 外志, 小田 實, 南雲 仁一, 志村 憲助
    1950 年 23 巻 6 号 p. 258-261
    発行日: 1950/01/01
    公開日: 2008/11/21
    ジャーナル フリー
    The apparent molal volume of amino acids in aqueous solutions increases with the ionic strength of other simple ions. The relation between molal volume and ionic strength was explained according to Kirkwood's theory. From the fact that this relation is equal for various α-amino acid, it was concluded that the hydration of hydrocarbon part was not accompanied with the electrostriction. Thus the two kinds of hydration are epxected for amino acids, or, more generally, for all water-soluble organic substances.
  • (第3報)一新Amide “Theanine” に就て
    酒戸 彌二郎
    1950 年 23 巻 6 号 p. 262-267
    発行日: 1950/01/01
    公開日: 2008/11/21
    ジャーナル フリー
    The author discovered a new amide in the water extract of Japanese green tea, and named it “theanine”. This substance crystallizes in the form of colorless needle; molecular formula C7H14O3N2, melting point 217_??_218° (decompose), and [α]12 D=+7.1. It presents strong ninhydrin reaction. It is very soluble in water, and is dissolved in 2.6 times of water at 0° and 1.8 times of water at 100°, but insoluble in ethyl alcohol and ether. By hydrolysis of theanine, it gives L-glutamic acid and ethyl amine nearly quantitatively. From the fact that it shows no biuret reaction and that the natural glutamine is γ-amide, it seems very probable that theanine may be L-glutamic acid γ-ethyl amide. It is the first time that ethyl amine has been discovered, though not in a free state, in plant kingdom. The author has also studied the distribution of theanine in each stage of tea leaf developments. Gyokuro is rich (about 1%) in it, but Sencha is poor. It seems, therefore, that theanine assumes the analogous rôle of glutamine or asparagine in other plants though the destination of ethyl amine is yet unknown.
  • (第4報) Paltreubinの分離
    酒戸 彌二郎
    1950 年 23 巻 6 号 p. 268
    発行日: 1950/01/01
    公開日: 2008/11/21
    ジャーナル フリー
    The author recently isolated a colorless needle crystal in the unsaponifiable part of ether extract of tea. Molecular formula of this substance is C30H50O2, m. p. 257_??_259°, and does not give Liebermann's reaction. It closely resembles paltreubin of JUNGFLEISCH and LEROUX (1906) as shown in the table above. Therefore, it seems to be paltreubin itselfmost probably.
  • (第5報) Theanineの合成
    酒戸 彌二郎, 橋爪 斌, 岸本 安生
    1950 年 23 巻 6 号 p. 269-271
    発行日: 1950/01/01
    公開日: 2008/11/21
    ジャーナル フリー
    Previously, one of the authors discovered a new amide in the water extract of Japanese green tea and named it theanine which was assumed to be L-glutamic acid γ-ethyl amide. Now we attempted to synthesize it according to the following scheme.
    L-Glutamic acid→L-glutamic acid γ-ethyl ester
    N-cbz-L-glutamic acid γ-ethyl ester
    N-cbz-L-glutamic acid γ-ethyl amide
    →L-glutamic acid γ-ethyl amide.
    L-Glutamic acid γ-ethyl amide obtained here had m. p. 217_??_218° (decompose) and [α]20D=+7.0. The mixture with natural theanine (m. p. 217°) showed no depression of its m. p. Their Cusalts were also completely identical. Therefore, it is provedd that natural theanine is L-glutamic acid γ-ethyl amide itself.
  • (第3報) Phosphorylaseの乾燥標本の調製
    丸尾 文治
    1950 年 23 巻 6 号 p. 271-274
    発行日: 1950/01/01
    公開日: 2008/11/21
    ジャーナル フリー
    Dry preparation of potato phosphorylase was prepared by dioxane precipitation. One part of phosphorylase solution was poured into two parts of dioxane, and the precipitate was centrifuged and washed by dioxane and ether. Then the precipitate was dried in a vacuum desicator.
    Phosphorylase action of this dry preparation was comfirmed by a) iodine color reaction of synthesized starch, b) formation of inorganic phosphate from CoRI-ester, c) effect of starch on the rate of formation of inorganic phosphate from CoRI-ester and d) formation, of CORI-ester from starch and inorganic phosphate. This dry preparation is stable and kept its action for two years at room temperature.
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