巻号一覧
29 巻, 12 号
選択された号の論文の21件中1～21を表示しています
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(第3報) Infusoriaの人工培養に就て(其の2)神立 誠, 高橋 直躬1955 年 29 巻 12 号 p. 915-918
発行日: 1955年
公開日: 2008/11/21
ジャーナル フリーFollowing the previous report, the experiments on the artificial culture of Infusoria from goat's rumen have been undertaken. The life span of Infusoria including Entodinia mainly, studied here, can be extended for about twenty days in vitro by addition of fresh white clover leaves at an amount of 5_??_6%; to the hay-RINGER'S extract culture medium. which is inoculated with rumen bacteria and incubated for 3 days.
It is observed that Infusoria are inan actively motile state and have frequent fission.
Infusoria can not survive at various concentrations of the clover juice in the absence of the other essential factors.
These effects of fresh white clover leaves on Infusoria cultures are not due to their inorganic substances.
When the fresh clover leaves are heated at 100° for 30 minutes, they do not forfeit their properties. So, it may be assumed that the effetcive factor of the clover leaves is a thermostable one. The same effective factor also exists in green peas and spinach leaves.抄録全体を表示PDF形式でダウンロード (1063K) -
稲神 馨1955 年 29 巻 12 号 p. 918-921
発行日: 1955年
公開日: 2008/11/21
ジャーナル フリーTryptophan metabolism of both normal and mutated silkworms were studied, and anthranilic acid, anthranilylglycine, sulphate of 3-hydroxykynurenine, glucuronide of 3-hydroxykynureine and 3-hydroxyanthranilic acid were detected -in the urine of the normal silkworm pupae.抄録全体を表示PDF形式でダウンロード (341K) -
稲神 馨1955 年 29 巻 12 号 p. 922-926
発行日: 1955年
公開日: 2008/11/21
ジャーナル フリーThe investigation of tryptophan metabolism in the silkworm with mutants related to this metabolism (ω1, ω2, rb), has shown that tryptophan metabolism of the silkworm differs a little from that of the mammal or microorganism.
A new tryptophan metabolite induced from 3-hydroxyanthranilic acid has been discovered.抄録全体を表示PDF形式でダウンロード (1075K) -
稲神 馨, 須藤 芳三1955 年 29 巻 12 号 p. 926-930
発行日: 1955年
公開日: 2008/11/21
ジャーナル フリー3-Hydroxykynurenine is found to be abnormally accumulated in the body fluid of a silkworm mutant called “Aka-Aka” (rb) which we. have discovered.
This abnormal accumulation in rb is caused by the inhibition of the metabolic courses in the normal silkworm, i.e. from kynurenine to anthranilic acid and from 3-hydroxykynurenine to 3-hydroxyanthranilic acid.
It may therefore be concluded, that the rb gene controls kynureninase, which has relation to this metabolism.抄録全体を表示PDF形式でダウンロード (445K) -
大村 京生1955 年 29 巻 12 号 p. 931-936
発行日: 1955年
公開日: 2008/11/21
ジャーナル フリーAscorbateの存在下pH7.5で調製したchloroplast fragmentsはphotosynthetic phosphorylation, oxidative phosphorylationの双方の活性を示す.このphotosynthetic phosphorylationの活性はaerobicでビタミンK3を添加しなくても極めて高く,酸素の存在. pyridine nucleotidesの添加に影響されない.抄録全体を表示PDF形式でダウンロード (1134K) -
(第3報)固定炉の恒温装置河野 哲郎, 佐藤 恵子, 鈴木 瑞枝, 磯部 育子1955 年 29 巻 12 号 p. 936-938
発行日: 1955年
公開日: 2008/11/21
ジャーナル フリーA new apparatus to keep the temperature of the high temperature furnace was designed. The circuit of SCHÖNIGER was modified as shown in Fig. 1. Furnace. B (about 200°), with a bimetal switch, was connected parallely with the high temperature funace (furnace A). The electricity would be supplied to the taps of 0_??_100V and 0_??_120V of the slide regulator alternatively so as to keep the temperature of the furnace A constant as wall as that of the furnace B, which was controlled by the bimetal switch. The temperature of the furnace was stabilized satisfactory as it was shown in Fig. 2. The ripple of the temperature at 1100° was about ±5°. The durability of the apparatus was more adequate than that of SCHÖNIGER's.抄録全体を表示PDF形式でダウンロード (266K) -
(第4報)ブランク値の変動の防止河野 哲郎, 佐藤 恵子, 鈴木 瑞枝, 磯部 育子1955 年 29 巻 12 号 p. 938-942
発行日: 1955年
公開日: 2008/11/21
ジャーナル フリーThe nitrogen purification unit and the main reaction unit in the apparatus for the micro-detemination of oxygen were examined and modified as shown in Fig. 1. A flow meter (Fig. 2). and a nitrogen tank which supplies a constant pressure were used to fix the analytical conditions. The temperature of the long furnace was also fixed with the stabilizer reported in the part 3 of this series(3). Nitragen gas was purified by passing it through the purification unit in the following way Copperoxide at 550_??_600°→Reduced copper at 450_??_500°→Reduced copper at room temperature→Silica gel→Calcium chloride→Ascalite→Anhydron.
The reduced copper layer at room temperature was necessary, because the copper layer heated at 450_??_500° could not eliminate the last trace of oxygen, though the rate of the reac-tion was faster at a higher temperature.
Content of the oxygen in the nitrogen thus purified was estimated as shown in Table 1, and it was concluded that oxygen could be eliminated almost completely by this proposed method.
The standard deviation of the blank values at operating conditions without any sample was reduced almost to the half that of obtained in the previous apparatus(2) (Table 2). Mean values of the blank values differed when different reaction tubes with different reagents were used. But the values were comparable with the data of other workers (Table 3).抄録全体を表示PDF形式でダウンロード (480K) -
(第5報)銀網を用いる新定量法河野 哲郎, 佐藤 恵子, 鈴木 瑞枝, 磯部 育子1955 年 29 巻 12 号 p. 942-946
発行日: 1955年
公開日: 2008/11/21
ジャーナル フリーTwo modifications for the microdetermination of oxygen were proposed. Oxygen in the sample was converted to carbon mono oxide by the usual method, i.e. the pyrolysed products of the sample was passed through a layer of charcoal heated at 1100±5°.
1) Carbon monooxide was then oxidized with anhydroiodic acid (Hl3O8) and the amount of iodine liberated was determined gravimetrically, after it was caught by a silver net roll which was heated at 450_??_500°. The procedure is much simpler than that of UNTERZAUCHER's, yet the conversion factor is as profitable as that of his, i.e. 1mg iodine corresponds to 0.3152mg of oxygen, and the results of determinations were. satisfactory.
2) It was found that the samples which contained sulfur could be analysed satisfactory when the gas bubbled through water prior to contact with anhydroiodic acid. Hence a water bubbler was equipped between the ascalite scraper and anhydroiodic acid. It was supposed that the moisture might prevent the reaction between anhydroiodic acid and CS2 or COS.
Note. a) The oxydation furnace and the anhydroiodic acid furnace must be placed 1 cm apart to prevent the overheating of anhydroiodic acid. b) Ascalite scraper must be renewed within a month, otherwise a larger oxygen value would result. c) Anhydroiodic acid is pre-pared in the following way: Dissolve iodinepentaoxde (I2O5) in a little amount of water and filter the solution while it is hot. To the syrup, at room temperature, be added concd. HNO3, to give a final concentration of 68 vol/vol%, and mixed homogeneously. Heat the solution at 100°. Crystals of anhy lroiodic acid would appear gradually. About 80% of the reagent would be recovered in the first crop. The reagent is not hygroscopic and does not react with hydrogen.抄録全体を表示PDF形式でダウンロード (491K) -
小幡 彌太郎, 石川 芳典, 吉田 利男1955 年 29 巻 12 号 p. 947-949
発行日: 1955年
公開日: 2008/11/21
ジャーナル フリーThe fractionation procedure of the phosphorylase inhibitor in sugar beet is improved as shown in Diagrams 1 ahd 2. After fractional precipitation with ammonium sulfate, the sugar beet, components are divided into 8 fractions by the use of ethanol, acetone, chloroform, methanol and ethyl ether. The inhibition (%) is proportional to the amount of the inhibitor in the range of 10_??_40% (Fig. 1).
After hydrolysis of most of the inhibitory fraction (80_??_90% EtOH fraction, shown in Table 2), only one reducing sugar, namely arabinose, and an ether-soluble substance, m.p. 139_??_140° are obtained. For the latter, the LIEBERMANN-BURCHARD's reaction shows a reddish-violet-violet color.抄録全体を表示PDF形式でダウンロード (261K) -
近藤 民雄, 伊藤 博之, 須田 元茂1955 年 29 巻 12 号 p. 950-952
発行日: 1955年
公開日: 2008/11/21
ジャーナル フリーAn investigation of the bark of Aesculus turbinata BLUME has revealed the existence of fraxin, aesculin, and cerin. The latter compound is a monohydroxy ketonic triterpenoid, previously studied by DRAKE et al.抄録全体を表示PDF形式でダウンロード (297K) -
(第4報)トチノキ心材の抽出成分近藤 民雄, 古沢 亘江1955 年 29 巻 12 号 p. 952-954
発行日: 1955年
公開日: 2008/11/21
ジャーナル フリーThe heartwood component of Aesculus turbinate BLUME has been investigated. β-Sitosterol and fraxin were isolated and identified. Three further compounds, i.e. aesculetin, fraxetin and glucose, were detected by the paper chromatographic procedure.抄録全体を表示PDF形式でダウンロード (278K) -
(第6報) (1) Phageに依る菌株の分別 (2) 所謂Sluggish FermentationとPhage作用との関係について木下 祝郎, 寺本 賢一郎1955 年 29 巻 12 号 p. 955-957
発行日: 1955年
公開日: 2008/11/21
ジャーナル フリー1. 3種のphageを用いて菌株の分別を行つた.その結果5型に分類可能である事が判明した.この中I型に属する菌株が最も多く, III型に属するものが最も少なかつた.
2. sluggish fermentationの原因としてphage MがKH 30号菌に対して作用しsluggishnessが再現し得る事を実証した. Phage Cも同様な作用が見られ,一般にlytic actionの弱いphage-host系では所謂“眠り”は生ぜずsluggish型を呈するものと解される.抄録全体を表示PDF形式でダウンロード (282K) -
(第2報)抹香鯨脳レシチンについて五十嵐 久尚, 座間 宏一1955 年 29 巻 12 号 p. 958-961
発行日: 1955年
公開日: 2008/11/21
ジャーナル フリーPhosphatides were extracted with petroleum ether from sperm-whale brain, and from these phosphatides were separated into lecithin and cephalin fractions with large amounts of alcohol. The cadmium chloride procedure was used for the purification of the lecithins.
The characteristics of these lecithins were found as follows.
P 3.61%, N 1.68%, N:P=1.03:1, Choilne 14.40%, N:Choline=l:0.98,
Iodine value 71.5
From the hydrolysate of lecithins, glycerophosphoric acid, choline and fatty acids were obtained.
These fatty acids were fractionated into solid and liquid fractions according to Twitchell's method; the latter fraction was separated into low- and high unsaturated fractions by the Li-Salts-acetone method. The solid (Table 2) and low unsaturated fatty acids (Table 3) were analysed by fractional distillation of their methyl esters, and the high unsaturated fatty acid (Table 4) by fractional crystallization of bromide from organic solvents.
These lectihins contained the following acids:
Palmitic 15.45%, Stearic 14.0%, Arachidic 6.4%C22H46O2. 3.5%, C24H50O2. 5.3%, Oleic 23.7%, C20H40O 2.5% and high unsaturated fatty acid (mainly arachidonic 25.2%)
From these results it can safely be stated that there are differences between the fatty acid composition of sperm-whale lecithins and that of other animals.抄録全体を表示PDF形式でダウンロード (243K) -
(第3報)スケトウダラ脳レシチンについて座間 宏一, 五十嵐 久尚1955 年 29 巻 12 号 p. 961-964
発行日: 1955年
公開日: 2008/11/21
ジャーナル フリーCrude lecithins were extracted with 95% alcohol from the pollak brain.
The cadmium chloride procedure was adopted for purification of the lecithins.
The characteristics of these lecithins found were as follows:
P 3.69%, N 1.63%, N:P=1:1.002, Choline 13.43%, N:Choline=1:0.96,
Iodine value 92.6
From the hydrolysate of lecithins, glycerophosphoric acid and fatty acids were obtained. These fatty acids were fractionated into solid and liquid fractions according to TWITHCHELL's method, the latter fraction being further separated into both low and high unsaturated fractions by the Li-salts-acetone method. Each fraction was analysed by fractional distillation of its methyl esters (Tables 2, 3, 4).
These lecithins contained the following acids:
Myristic 4.3%, palmitic 19.5%, stearic 9.9%, arachidic trace, C14H28O2 1.6%,
Zoomaric 10.0%, oleic 32.9%, linolenic 0.4%, C20H32O2 6.9% and C22H34O2 14.0%.
From these results, it can safely be stated that there are differences between the fatty acid composition of pollack brain lecithins and that of other animals.抄録全体を表示PDF形式でダウンロード (388K) -
柳下 一愛, 伊勢田 駿1955 年 29 巻 12 号 p. 964-967
発行日: 1955年
公開日: 2008/11/21
ジャーナル フリーThe ethanolic extract of the dried bark of Platanus occidentalis LINN. contains a portion insoluble in water and a 10%, NaOH solution. From the ether-soluble part (insoluble part 0.38%) of this portion, hydroxytriterpenic acid was obtained via its potassium salts (1.85%). The free acid (1.72%) was recrystallized from ethanol, giving colorless needles, m.p. 310_??_311°, which were proved to be identical with betulinic acid isolated from the calyx of Japanese persimmon. The ether-soluble neutral substance (0.75%), after hydrolysis, yielded sitosterol and stearic acid. From the 10% NaOH-soluble portion, three acidic parts were separated extracting the freed acidic ethereal solution with Na2HCO3-, Na2CO3- and NaOH-solutions. Yields were 0.01%, 0.02% and 0.02% respectively. Besides these, 0.08% of the neutral part was also obtained. From the heartwood of the same plant colorless needles were obtained, m. p. 310_??_311°, which were proved to be identical with betulinic acid by admixing them With the authentic specimen.抄録全体を表示PDF形式でダウンロード (403K) -
高井 康雄, 小山 忠四郎, 加村 崇雄1955 年 29 巻 12 号 p. 967-972
発行日: 1955年
公開日: 2008/11/21
ジャーナル フリーThe metabolic pathway of microorganisms, which are responsible for the development of reductive conditions, in water-lodged paddy rice soils was pursued by the estimation of Eh7, Fe++/Fe+++Fe+++ number of various bacteria, NH4+, NO3-, S--, gaseous components (02, CO2, N2, H2, CH4) and organic acids contained in the reduced layer. The results obtained were as follows:
(1) In the earls stage of incubation process, Eh7 dropped rapidly and Fe++ was reduced, both aspects of these transformations thus forming a contrast.
(2) The peaks of bacterial number followed in the order: firstly bacteria by plating, secondly those by the deep tube method and lastly sulfate reducing bateria after incubation.
(3) Nitrate disappeared in the initial stage of incubation, and ammonia was liberated along with the progress of reduction.
(4) After the reductive conditions reached a certain level, active sulfide formation initiated.
(5) Speaking about gaseous metabolism, O2 disappeared within 1 day of incubation. Later, CO2 was evolved and accumulated from 4 to 9 days. After vigorous accumulation of CO2, the decrease of CO2 accumulated and evolution of CH4 occured contrastly, and H being concur-rently evolved.
(6) The amount of organic acids produced within an incubation period of 2 to 9 days markedly decreased later.
From the above results, it may be concluded that this is the first attempt made to typify respe_??_tive metabolic steps in the water-lodged paddy soils. This may be stated as follows:
The 1st step: oxybiontic metabolism by aerobes or faccultatively anaerobes.
The 2 nd step: unoxybiontic metabolism by faccultatively or strictly anaerobic heterotrophs.
The 3 rd step: methane fermentation and sulfate reduction, in which CO2, H2, organic acids or alcohols, produced in the foregoing 2 steps or this step were consumed as metabolites.抄録全体を表示PDF形式でダウンロード (522K) -
(第3報) E. coliのCell Suspension及びCell-free Extractsによるα-Ketopantoci acid及びPantoic acidの生合成松山 晃1955 年 29 巻 12 号 p. 973-977
発行日: 1955年
公開日: 2008/11/21
ジャーナル フリー(1) The enzymatic, formation of a-ketopantoic acid from, β, β-dimethylpyruvic acid and formic acid by the cell suspension and cell-free extracts of Escherichia coli was shown in Table 1 and Table 2, respectively, and further confirmatibn was made by means of paperchromatography of the enzymatic reaction mixture.
(2) As the cofactors of this reaction leucovorin, V. B6 (pyridoxal ), V. B12. and ATP were effective.
(3) It was presumed that the function of folic derivatives in one-carbon transfer was attributable to their labile formyl group, and the stages requiring both V. B6 and V. B12 or each individually, differed from those catalyzed by folic acid derivatives.
(4) ATP might be probably necessary in the reaction
FAH4→FAH4-CHO.
(5) As one-carbon source of this reaction formic acid was more effective than formalde-hyde or methanol.抄録全体を表示PDF形式でダウンロード (455K) -
(第4報) α-Ketopantolactoneの2. 4-dinitrophenylhydrazoneの分光学的検討(その1)松山 晃1955 年 29 巻 12 号 p. 977-982
発行日: 1955年
公開日: 2008/11/21
ジャーナル フリーKPL-DNPの〓吸収帯について次のことを明らかにした.
(1) 3500～3000cm-1のregionには～3300cm-1, ～3200cm-1及び～3100cm-1に各1乃至2本の吸収帯かおり, A異性体では～3330cm-1, B異性体では～3200cm-1に比較的強い吸収があり, C-N結合に関して前者がanti型,後者がsyn型の幾何異性体であると推定される.
(2) 稀薄四塩化炭素溶液のスペクトルから，これらの吸収にはいずれも分子間水素結合が影響していないと考えられる.
(3) 両異性体にみられる～3100cm-1の吸収はaromatic vc-H,吸収帯である.
(4) 四塩化炭素の稲荷溶液中で加熱することによりvc-H吸収帯に著しい変動かおこり,～3300cm-1の吸収は消滅し,新たに～3030cm-1に相当顕著な吸収が出現すること,及びこれがプロトンの転移によるN…H-O型分子内水素結合の生成にもとつくものと推定されることを明かにした.
(5) anti型にみられる～3200cm-1の吸収帯はdipicrylamineにもみられ,これは-C6H3(NO2)2乃至-C5H2(NO2)3のような強い電子吸引効果をもつ基が置換した>N-H結合にふるものと考えられる.抄録全体を表示PDF形式でダウンロード (504K) -
(第4報) α-Ketopantolactoneの2, 4-dinitrophenylhydrazoneの分光学的検討(その2)松山 晃1955 年 29 巻 12 号 p. 982-985
発行日: 1955年
公開日: 2008/11/21
ジャーナル フリーBoth VC=O bands in the infrared absorption spectra and the ultraviolet absorption maximum of 2, 4-dinitrophenylhydrazone of α-ketopantolactone (KPL-DNP) have been studied and the results obtained are as follows:
(1) In the region 1800_??_1700cm-1 each KPL-DNP isomer show splitting VC=O bands. With the solution spectra, VC=O bands appear at _??_1775cm-1 and _??_1745cm-1.
(2) In comparison with the spectra as Nujol mull, absorption at _??_1745cm-1 with anti-isomer and _??_1775cm-1 with syn-isomer weakens relatively in chloroform solution and strengthened in pyridine solution. From these facts the relationship between one of the splitting bands and the polarity of solvents may be suggested. Therefore, the splitting of VC=O bands may be attributed to the intermolecular interaction.
(3) In the ultraviolet absorption spectra, a shift of the maximum of KPL-DNP (near 350mμ) toward longer wave lenghth in the polar solvents is observed. Thus, the quinoid structure probably contributes to the excited state of this compound.抄録全体を表示PDF形式でダウンロード (332K) -
(第5報) 2-ケトグルタールアルデヒドの合成(其の一)麻生 清, 杉沢 博, 磯 俊男1955 年 29 巻 12 号 p. 985-987
発行日: 1955年
公開日: 2008/11/21
ジャーナル フリーフルフラールから2, 5-diethoxy-tetrahydrofurfural diethyl acetalを合成し,これを0.1N HClで開環させて2-ketoglutaraldehydeをtrisemicarbazoneとして得た.抄録全体を表示PDF形式でダウンロード (281K) -
小出 英興, 長山 英男, 志村 憲助1955 年 29 巻 12 号 p. 987-990
発行日: 1955年
公開日: 2008/11/21
ジャーナル フリー1) 5令中の蚕の絹糸腺,消化管及び脂肪体の各組織中には種種のtarnsaminaseが存在することを明らかにした.しかし体液ではtransaminase作用は認められなかつた.
2) 5令中transaminase活性度の変化はいずれも同様の傾向を示し, 5令4日前後に於て最も強く, 5令初期及び熟蚕期には低下している.この傾向は絹糸腺に於ける絹蛋白質の合成速度と平行するようである.
3) 19種のアミノ酸とpyruvic acid, oxalacetic acid及びα-ketoglutaric acidの3種のα-ケト酸との間の反応を絹糸腺組織について検索し, glntamic-pyruvic, glutamic-oxalacetic及びaspartic-pyruvic transaminaseの3種が特に顕著であるが,その他のアミノ酸に関するtransaminase作用も明らかに認められ,これらの各種のtransaminaseは蚕体内でのアミノ酸の桐互転換に重要な役割を演じているものと推定される.抄録全体を表示PDF形式でダウンロード (365K)
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