The author previously reported a remarkable transformation of a mature rhizoidal cell of
Nitella flexilis into an internodal one as a result of isolation from the rest of the plant and of culture
in vitro. This metamorphosed cell was developed to a small plant consisting of 'leaf' cells, internodal cells, nodal cells and rhizoidal cells.
In the present experiments, the rhizoidal cells cultured with the rest of the plant were also transformed to have the shape of the internodal cell, containing many nuclei and chloroplasts. Furthermore, shoots and rhizoids were newly formed from these metamorphosed cells. The suitable illumination for this metamorphosis was about 500lux with fluorescent light and adequate pH was around 6.6. Some plant growth hormones, vitamins and amino acids, e.g., IAA, NAA, vitamin B
2, C, aspartic acid and glutamic acid, promoted this metamorphosis at appropriate concentrations.
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