In order to answer the question, which is the essential component of Perilla-anthocyanin, 'perillanin' or 'shisonin', leaf extracts from two common varieties of the plant were subjected to paper chromatographic study. The results have shown that the anthocyanin is nothing other than 'shisonin', i.e.p-hydroxycinnamic acid derivative of 3 : 5-o-diglucosidylcyanidin, as described by Kuroda and Wada. There was found no evidence in favor of the presence of Kondo's 'perillanin'.
1. The sugar in Lycopus lucidus Turczaninoff were investigated by means of paper partition chromatography. 2. Glucose and galactose were present in all parts of the plant. Fructose was confined only in the stolon. 3. In addition to the monosaccharides and a hexasaccharide lycopose, sucrose, raffinose, and stachyose were found.
Anatomical and morphological studies have been carried on the sporophytes, especially on the leaves, of Cheiropleuria bicuspis var. integrifolia from Hachijo Island. Gametophytes which persist imperfectly on some juvenile plants are also observed. There is an abundance of particular and primitive characteristics, such as, (a) the protostele showing a dorsiventrality in the dominant number of the protoxylem groups on its dorsal side ; (b) a marked dimorphism shown as the sterile and fertile leaves ; (c) firm and leathery laminas which are frequently bicusped at their tips and are provided with 4-6 parallel veins formed by repeated bifurcations of bundles of Marattia-type in the nor-articulated and wiry petiole, etc. An assortment of such characteristics of the sporophytes apparently shows an independent systematic position of Cheiropleuria among the Leptosporangiate ferns, and, thus, strongly supports the opinion of Nakai, who established an independent family Cheiropleuriaceae on the basis of some particular characteristics of the gametophyte.
1.The karyotypes of five species and three varieties in Ranunculus are determined as follows: R. japonicus K(2n)=14=2A1m+2A2m+2Bm+2C1st+22Cst+2C3st+2tDst R. yakushimensis K(2n)=14=2A1m+2A2m+2Bm+2C1st+2C2st+2C3st+2tDst R. auricomusK(2n)=28=4A1m+4A2m+4Bm+4C1st+4C2st+4C3st+4tDst R. ternatus var. glaberK(2n)=16=2A1m+2A2sm+2A3sm+2B1st +2B2st+2Bst+2Cst+2Dst R. ternatus var. quelpaertensisK(2n)=16=2A1m+2A2sm+2A3sm+2B1st +2B2st+2B3st+2Cst+2Dst R. cantoniensisK(2n)=32=4A1m+4A2sm+4A3sm+4B1st +4B2st+4B3st+4Cst+4Dst R. sceleratusK(2n)=32=12Am+4Bm+12Cst+4Dst R. reptans var. flagellifoliusK(2n)=32=12Am+4Bsm+16Cst 2. Comparing the 7-basic complement in fig. 1 with the 8-basic one in fig. 3, we find the former to differ markedly from the latter in the absence of a chromosome corresponding to h in fig. 3 and the presence of a satellite g in fig. 1. 3. From the karyotype analysis, the race with the basic number 8 is divided into two groups. The first includes R. cantoniensis and the two varieties of R. ternatus, and the second R. sceleratus and R. reptans var. flagellifolius.