It was researched how the tissue of the skin suffered changes when it was soaked in the lime solution. Tne results are as follows:
1 In case the raw skin was soaked in the saturated solution of lime, every cellular element of skin took various forms to be dissolved, and most of them vanished within 72 ho us. The cells of sweat glands and erectorpili muscles were the earliest and every cell in epidermis the next and the cell in hair follicle the last o be solved.
2 The keratin substance which composed epidermis was dissolved in the lime solution. But layers in epidermis separated not individually from one another, but together from the part of the corium. In this case the internal part of the epidermis melted and became one membran like plate.
3 The kittsubstances among the tissues were dissolved early, and the tissues became to be in isolated states. When this state was over, the remaining tissues(collagen fibres)swelled and occupied the space which was produced by the separation.
4 The collagen fibres began to swell immediatly after soaking, and increased the degree of expansion as time passed, but began to separate from one another after about 72 hours owing to the dissolution of kittsubstance. At the same time the fine fibres. also began to swell and continued to separate from one another for about 7 days. Afterwards the spaces was not recognized because of the swelling. The collagen fibres in papillar layers was earlier in separation and expansion than those in reticular layers.
5 The elastin fibres swelled little by little, and the changes in the form were not recognized so clearly.
6 Some of the cells of sebaceous gland made little change by lime soaking, but the nucleus in that cell made a change in form or was dissolved. The cytoplasma of the cell in raw skin were not able to be stained by acid fuchsin, but they were stained when soaked in lime solution.
7 The medulla of hairs did not make so much in change as others did. But the cells surrounding to support the hairs were dissolved, and so the hairs were isolated from one another into a floating condition. Moreover, this floating condition was thought to be enlarged by the spaces of sweat glands. Nevertheless, the hairs became fixed by the pressure of the swelled collagen fibre because of the soaking in the lime solution for a long time.
8 Accordidg to the facts mentioned above, it was thought to be best to dehair after the hairs were soaked for 4 or 7 days in lime solution at-18°C. In comparison with various former theories of dehairing it was proved that the chief cause of dehairing was the change in the form, produced by collapse of the tissue.
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