In the previous paper the author, in a clinical approach, reported on the `difference between hookworm anaemia and anaemia of the blood donors. And also investigation was made on anaemia of dogs infected by the 4th stage of larvae.
In this study the author prepared cultured fluid which consisted of metabolic substances and excreted products of the 4th stage larvae. The fluid was injected into the gluteal muscles of the rabbits, the blood picture of which were examined. The results are as follow:
1) In 4-5 hours after the injection a remarkable decrease of erythrocytes leucocytes and haemoglobin was noticed. This decrease recovered to almost the normal level within 48 hours.
2) As for the percentage of the type of leucocytes 4-5 hours after the injection, neutrophils increased but lymphocytes decreased. This identifies the inhibition or fatigue of the bone marrow that has been described by Schilling.
3) In the controls, no change was seen.
According to this result and to the previous report, it is considered that hookworm anaemia is induced not only by bleeding but also by excreted products of the worm body.

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In order to study prognosis of splenectomy, 15 patients, 10 females and 5 males with banti's syndrome, who had been undergone splenectomy were observed particularly on their liver functions in the period of long times.
Some patients complained of post-operative exhaustion or fatigue, but there was no tendency noticed to get illness, for instance common cold, etc.. The liver function tests were made every three months. They were B. S. P. Retention, C. C. F., Cholinestelase activity, total cholesterol, alkaline phosphatase, prothrombin time.
The results are as follow:
Including B. S. P. the prognosis of the post-operative liver functions most of the patients indicated worse results. While by blood test recovery from anaemia was noticed and this condition persisted for a long time. The serum protein increased. In 15 cases an interesting relation was found between the liver function and blood picture, the more increased the hemoglobin, the better the results of the liver function.
Among patients of over 10 years after onset and of less than 10 years after onset the former have better prognosis.
From these results obtained, it is seemed necessary a thorough protection liver regiem be applied before and after the operations.

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³²p (Phosphoric acid) contaminated lactose mixed acetylsalicylic acid or other several drugs. Investigated distribution of the radioisotopes labeled lactose after vibrated bottle about surface and bottom layer.
Each ratio combination changed by vibrating for 30 minutes (120 vibrations per minute).
These changes were more variable heavy specific gravity than light specific gravity.

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First the author classified pulmonary tuberculosis patients into two groups: Patients without any aggravations in their anamnesis belonged to the “better group” and patients who have had aggravations in the anamnesis belonged to the “worse group”.
Secondly, according to the degree of improvement of their chest x-ray photographs pulmonary tuberculosis patients were classified into two types the “better type” and the “constant type”.
The thyroid functions were studied on each cases individually.
Furthermore, in proportion as the confidence limit in PBI (protein bound iodine) rate among healthy human beings, the PBI rate among the pulmonary tuberculosis patients were classified into three groups; higher group (more than 5.85γ/dl), the ordinary group (5.85-5.22γ/dl) and the lower group (less than 5.22γ/dl).
1) It is interesting that the individual FBI rate is far more widely distributed among the worse or the constant groups than among the better group. The cases of their higher PBI group showed no difference between better, worse and constant groups. However, in the “ordinary” cases, more difference was noticed in the better group than in the worse.
And the cases of lower group more difference was noticed in the “worse” category than in the better whereas the constant type had trends to be found more frequent than the better type.
2) The significant difference is noticed that individual basal metabolic rate shows wider distribution in the worse and the constant than that of the better.
3) The significant difference is also noticed that each I¹³¹ up-take rate by the thyroid gland after 1, 3, 5 and 24 hours shows wider distribution in the worse and the constant than in the better. Especially after 24 hours all cases of the better group are found to be within normal range. While some cases of either worse group or constant type are not found within the normal range.
Summarily, among the pulmonary tuberculosis patients, anamnesis with or without occurance of aggravation and the degree of improvement which is found on the chest x-ray photographs varies the thyroid function.
It seems that the function is more unstable among the worse group and the constant type than that of the better group.

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Susceptivility for PZA on H37Rv in vitro was investigated. For this purpose various kind of hydrogen liquid or solid nutrient medium were applied.
1) With solid media (KH₂PO₄ media) under pH 7.5-5.5 by 10^-1mg demonstrated complete resistance at 3, 000γ, under pH 5.0 to 50γ. However, by applying 10^-4mg indicated under pH 7.0 at 3, 000γ, under pH 6.5-5.5 to 1, 000γ, and under pH 5.0 to 10γ. This was decided at the number of 1/3 colonies against the contrast.
2) Liquid media (Kirchner media) - under pH 7.0-6.0 by 10^-1mg, growth of the bacillus were seen up to 3, 000γ.
However, under pH 5.5 growth was demonstrated up to 1, 000γ and under pH 5.0 inhibition of bacillary growth was at 10γ. When 10^-4mg of bacillus was inoculated the growth was variable dependent upon pH, pH 7.0 to 3, 000γ, pH 6.5 to 1, 000γ, pH 6.0 to 3, 000γ and under than pH 5.5 showed no growth.
This indicated that the inhibition of growth of tubercle bacillus was seen under the following conditions : the greater H37Rv quantity the higher concentration needed, and the more acidity the media inclined the lower concentration needed.
The INH was added to the above mentioned kinds of nutrient medium and the similar test was made.
1) Solid media with 0.01γ/cc INH - under pH 7.0-6.0 demonstrated complete resistance at 1, 000Wγ, under pH 5.5 to 50, and pH 5.0 to 10.
2) Liquid media demonstrated the similar phenomenon.
This indicated that a synergic relation exists between PZA and INH.
For the purpose to study variety of susceptivility for PZA, investigation was made on two different conditioned media: 3% K₂PO₄ media with pH ca. 6.2 resisting up to 3, 000γ and 6% KH₂PO₄ media with pH ca. 5.5 resisting up to 1, 000γ. And 57 patients who had been unsuccessfully treated by SM, PAS and INH were tested.
The results are as follow:
1) Before their chemotherapies, the case of emergence of PZA-resistant strain was none.
2) One month after their therapies the percentage of emergence was 33.3.
3) After 3 months 73.7% and after 6 months 89.2%.
4) Compare with the INH-sensible strain, the INH-resistant strain had higher percentage on the emergence of the PZA-resistant strain. And this difference was noticed particularly in the early stage of the therapy which was estimated at least less than 4 months.
5) Six months' PZA-INH therapy showed decrease of both the SM resistant and PAS resistant-strain.

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In this paper the secondary reactions followed by chemical treatment with a lame amount of INH are reported.
The experiments are made on DD strain mice, Aoyama bacillus, and INH. The nutrition consists of starch, milk casein, soy bean oil, McCallum salt and panvitamins.
Following results are obtained
When a large amount of INH (Iscotin pro kilo 250mg) are given to mice, remarkable difference were noticed in their mortality; the mice with high protein diet showed 25% of their death rate, while the mice with low protein diet showed 100%.
This result indicates the necessity of protein diet in the nutrition of TB mice.

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Into the white of the hen's eggs (white Leghorn) Al mg and 1.0 mg of virulent human type of tubercle bacilli “Kiyo HI” were inoculated The chickembryos were harvested 4 times, in 12th, 15th, 18th and 21st days following the beginning of incubation.
The quantitative measurement of tubercle bacilli colonies by isolation culture in the liver and the lung, and the histologic examination for the two organs were made. The author obtained the following results.
1) It was considered that the tubercle bacilli “Kiyo HI” inoculated into the white were less virulent for the chickembryos than the tubercle bacilli inoculated onto the chorio-allantoic membran previously reported by foreign investigators.
2) Tubercle formation of the liver was observed in 4 cases out of 31 cases (129%), that of the lung being only one case (3.2%) from 15 days' incubation to 21 days' one in this study.
3) It was shown that tubercle bacilli of the liver in number were increased more than those of the lung.
4) Microscopic appearance of the liver was characteristic of monocytic and eosinophilic infiltration around the vessels and that of the lung was consisted of only the infiltration of eosinophils at the perivascular areas.
5) Influence of virulent human type of tubercle bacilli “Kiyo HI” for the chickembryos was found remarkably different between liver and lung. The difference of the tuberculous reaction in the two organs may be due to the affinity of each organs to tubercle bacilli.
6) It was proved that tubercle bacilli inoculated into the white of eggs invaded the body of the chickembryos following the development of vascular system through the time of incubation.

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