The Present experimental studies were conducted on the effect of hot springs upon amylase by means of “Wohlgemuth's 30 minuites test”. The results are summarized as follows;
1) Kaimonji hot spring had the activity of stimulating the power of diastase, but Takegawara and Yanagi hot springs had not.
2) It was found that the power of Amylase was weak under specific pH values of the hot springs (about 6.4-6.8), compared with the optinum pH, pH5.0,
3) When the hot spring waters were left in room temperature for one month after springing, Kaimonji hot spring showed a decrease of the stimulating activity upon the power of amylase whiles both Takegawara and Yanagi hot springs did not show any changes. Components of the hot springs decreased by 7% in Kaimonji, 8% in Takegawara and 14% in Yanagi, in the process.
4) When hot spring waters had been left for longer period, they evaporated until dry. The concentrated substances thus obtained were dissolved again in distilled water and used in the following experiments. The power of amylase by Wohlgemuth's method was weaker in the disolved solution than the fresh hot spring.
5) The more dilute hot springs had the stronger effect upon the power of amylase than the concentrated ones.
6) From the results mentioned above it was assumed that the principle effect of the Kaimonji hot spring upon the power of amylase was attributed to the “Fervoreffect” of Vouk.

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It is a matter of coarse that if blood taking preservation and transportation are done in accordance with the regulation of “Standard of citrate-added blood” transfusion will be safe. However, preservation of blood is very difficult. Recently we experienced 4 cases of bacterial coatamination of preserved blood and severe reactions. These symptoms were shock syndrome and peripheral vascular collapse. The Ist case was luckily saved, but the 2nd and 3rd were not. We conducted the examination of preserved blood and we found bacterial contamination. And yet these preserved blood were offered from a blood bank of which institution and technic are said to be excellent in our country; and we could find none of mistake in the preservation, transfusion, technic of transfusion and available period.
In our 4 cases, we isolated Gram negative bacillus by means of bacterial culture, and each of them were classified into Escherichia freundii, Paracolobactrum aerogenoides, Achromobacter and Flavobacterium. Morever, it must be notified that the multiplication of these 4 bacillus were better in room-temperature or ice-room than in 37°C; and the all of these 4 stains were ordinary plus by simple stain of smear of remained blood.
Having these unexpected accidents of 3 cases, we have a fear about the standard of the Welfare Ministry: “Blood must be examined with naked eye before distribution in the factory, and before transfusion in the clinic”. We could find the bacterial contamination by means of simple stain of smear of blood, while it was unable with naked eye to observe any abnormalities, especially in our cases.
From these results, we advocate the necessity of microscopy of simple stain of smear of blood, directly before transfusion.

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Cases of tuberculous lesions in contralateral lung, having pneumothorax in one side lung, were studied as to their changes of pulmonary tuberculosis in the opposite lung.
In case of bed rest, many cases became better and few became worse in pulmonary tuberculosis in the opposite lung.
In the cases of streptomycin therapy, recovery tendensy was greater than that of bed rest, as to the pulmonary tuberculosis of the opposite side. However in a longer observation, recovery ratios of these two cases became very close to each other and many cases showed remarkable recovery.
The bed rest played an important role in recovery of pulmonary tuberculosis in the opposite lung, while the chemotherapy increased the recovery ratio.
By a longer observation for 1-2 years, many cases recovered in their pulmonary tuberculosis of the opposite side, but only few cases showed remarkable recovery. It is necessary to conduct concentrated treatment in the first year.
The opposite lung must compensate the respiration efficiency of the lung which was under pneumothorax. However, during exercise or work the compensation will become much more, and the subsequent influence on the pulmonary tuberculosis should be cared.

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Electrocardiographies of pulmonary tuberculosis were reported by many investigators. While the electrocardiography (ECG) gives the electrical potentials of heart, the ballistocardiography (BCG), introduced by Starr, presents a part of cardiac forces. As Starrs high frequency type BCG-apparatus was so bulky that it was inconvenient for general Dock, devising an electromagnetic type has published many interesting results. By means of Dock's simple apparatus, the author has recorded simultaneously BCG and ECG curves in 272 patients with pulmonary tuberculosis, and compared the findings with their symptoms, classified as far advanced, moderately advanced and minimum. Results were as follows:
1) Both BCG and ECG were found normal in 42% of all the cases.
2) Abnormal BCG and normal ECG were found in 34%.
3) Normal BCG and abnormal ECG were found in 12%.
4) Both BCG and ECG were found abnormal in 11%.
5) Abnormal BCG were found most commonly in far advanced cases and least in minimum cases.
6) After Dock's classification, the first grade of abnormal BCG was most and the fourth grade was least in their number.
7) As to the abnormal curves, K-notching, slurring, marked L wave and early M pattern were found in most cases.
8) The numbers of frequency of abnormal ECG curves were in the following order myocarddamages; right ventricular conduction disturbances; incomplete bundle branch block.
9) Generally speaking, abnormal curves were found more in advanced cases and less in minimum cases.

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By means of the same method as in the inoculation of cl. perfringens into guinea pigs, staphylococcus aureus was inoculated to guineapigs, which showed series of symptoms of local purulent inflammation and fever in general after certain incubated period.
The virulence was prominent in staphylococcus aureus which has moderate activity of hemolysis and of plasmacoagulation and the virulence was rather unremarkable in staph. aureus which has less activity of plasmacoagulation.
The coccus which has no power of plasmacoagulation did not show any activity of provocating symptoms in the animal.
It would be concluded that the activity of plasmacoagulation is in proportion to that of pathogenesis, as previously mentioned by other authors. The author utilized these results as criteria for the evaluation of immunologic effect. There were no remarkable differences between spontaneous healing, cases treated with living or killed bacilli, except cases treated with toxoid. This exhibited slight prohibition of symptoms.
As to the evaluation of the power of antibiotics against the inoculated inflammatory foci, the animals died within a few hours after inoculation of penicillin, without rupture or incision of abscess, even though penicillin was small in amount. The patho-histologic findings were similar to that of experimental anaphylaxis.
It is not clear whether this result has any relationship to penicillin anaphylaxis.
Only the following possibilities would be taken in considaration; i. e., existence of common constituents in penicillin and in staph. aureus; inoxication of unknown substance produced by deterioration of bacteria by penicillin treatment.
If abscess developed into ulcer or fistula or if it was incised, the animal servived notwithstanding the inoculation of penicillin at the same time.
The intervening factors should be separated from each other, in the further study.
It is obvious that the inflammation foci due to staph. aureus, which has resistance against penicillin, will have rather less influence by penicillin.
Experiments of streptomycin performed in the similar manner did not give the above findings.
Experiments utilizing the other kinds of antibiotics will be reported in the future paper.

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In order to study the nature of anamie by ancylostomiasis the authors investigated morphological and physical changes of the blood and the bone-marrow from 19 patients with Ancylostoma dubini.
It was found that 79 per cents of the investigated cases showed 3 million level of blood cell counts. No evident relation was observed between the degree of anemia and number of expelled worms, although in many cases fewer worms were expelled when anemia was slight. The anemia was of hypochromic and microcytic nature. Number of reticulocytes in our cases was smaller than those in hemorrhagic anemia and larger than those in aregenerative anemia. There was no close connection between numbers of leukocytes and of expelled worms, nor any evident relation between acidophile percentage and expelled worms, nor between the anemia and occult blood reaction in stool, but there was some relation between occult blood reaction and expelled worms. Number of nucleated cells in bone-marrow was in normal range, and it seems noteworthy that when the anemia was of medium grade, nucleated cells tended to increase. The number of erythroblasts was also in the same relation as that of nucleated cells to the anemia. Basophilic erythroblasts in bone-marrow increased in proportion with anemia, whereas the polychromatophilic and normochromic increased when anemia was slight. Nucleus relatively matured in many cases. There was no tendency of increase of karyorrhexis subsequent to the exacerbation of anemia, but a picture of so-called maturearrest was observed. In leukocyte system, shifts to right or left wasnot distinct. No remarkable change was seen in thrombopoiesis. In bone-marrow tissues from severe anemia, slight edema and adiposis were observed. These findings were different from those in pernicious anemia by Rohr, which has mature-arrest. The etiology of ancylostomatic anemia can not be explained neither by hemorrhagic theory nor by hemolytic theory.

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