1) In the treatment with several anti-tuberculous agents, behaviours of acquired resistance were investigated. Resistance examination was conducted in the 4th, 8th, and lath month after the termination of the treatments of SM and PAS in combination, SM alone continuously, SM alone intermittently, PAS alone continuously, TB1 alone continuously and INAII continuously.
The results obtained were as follows In each case, except the cases readministered with the same drugs, the acquired resistance began to reduce or even disappear in the 4th month and almost vanished completely in the 18th month, however, a single case retained 100γy resistance against SM. Mode of administration was indifferent. Furthermore, the disappearance of higher resistance retarded than that of lower ones.
2) As for the resistance in relation to dosis and periods after the termination of the treatment, no cases acquired resistance in the 18th month for SM, and during 12 months for PAS, TBl and INAH.
3) Resistance was examined two times for SM, PAS, TB1 and INAH treatments cases. The cases to be investigated were classified into 3 groups, i. e., sensitive group, lOy group and 100γ resistant group, and further they were divided into 2 groups, i. e., groups treated with and without same and other drugs in the period between the first and second examination. In case no retreatment was applied, all the sensitive cases retained their sensitivity, and of the 10γ-resistant group for SM were sensitive. No less than half of the other groups also became sensitive. On the other hand, the 100γ-resistant groups for SM retained their resistance at the same degree except one case. In the retreated group with respective drugs, the sensitive cases showed remarkable increases in the resistance. The resistance of the 10-100γ-resistant groups also increased to the same or higher degree. When retreated with another drugs, all the sensitive cases were still sensitive and the 10-100γ-resistant groups became sensitive or gave the same degree of the resistance. No increased resistance was observed.

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Normal white male rabbits were injected with 5 cc of Ag-elecroid pull icc of physiological saline solution, two times at the interval of 24 hours, and the rate of pseudo-eosinophile cells, nuclear shift, pliagocytosis (indian ink particles), amounts of serum albumin and globulin, concentration of acid-fuccin in the aqua anterior, dead coli bacilli agglutinin value of serum and the aqua anterior were investigated. The results were as follows:
1) The phagocytosis of pseudo-eosinophile cells were accelerated by the injection of the said fluid and showed the maximum level on the lst-2nd day subsequent to the initial injection. It fell off to the original level by 7-16 days after the 2nd injection.
2) The amount of serum globulin increased by each injection and reached to the largest on the 1st-7th day subsequent to the 2nd injection. It returned to the original amount by 10 th day after the 2nd injection.
3) The counts of pseudo-ecsirophile cells increased gradually through the injections. They were highest figures on the 1st-3rd day subsequent to the 2nd injection and recovered their original figures by 6-9 days.
4) As for the nuclear shifts of pseudo-easinophile cells, left-shift was seen subsequent to the 1st injection; number of nuclei became minimum on the 1st-2nd day of 2rd injection; normal conditions were regained by 10 days after the 2nd injection.
5) The concentration of acid-fuccin in the aqua anterior decreased for 1-3 weeks subsequent to the injections.
6) Seven weeks after the preliminary immunization through dead coli bacilli, a group of rabbits were injected with the said fluid (Ag-elecroid). Their serum agglutinin increased by the 2nd week. No changes were seen in the aqua anterior.
7) Body weights and body temperature were not affected, practically.

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In the preceding two papers, the author reported on the quick and quantitative determination of the drug sensitivity of TB bacilli. As for the clinical applicaticn cf this method, the author conducted the measurement of the sensitivity of TB bacilli, isolated from TB patients, who had been treated with SVI, PAS, TB₁, INAH, singularly or in combination. The results were as follows:
In siingle adninistration, the resistant strains of the concentration of above 10 mg per ml were generally measured when the administration of SM amounted at 30g, PAS at 530g, TB
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, at 6g and INAH at 16g. In proportion to the increase of dosis, the grade of the drug-resistance and its frequency increased, On the other hand, when the drugs were administered in cornbination, the appearance of the resistant strains was inhibited or considerably delayed' Even if the resistant strains developed in the combined administration, the grade and frequency of drug resistance were smaller than in case of single administration. Incidentally, antituberculous drugs shall be administered in combination, in order to inhibit or delay the appearance of the resistant strains.
The quick and quantitative determination of drug ersitivity cf TB bacilli through the slide culture method has a great clinical value in the reliability of its results and its simplicity of technique.

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In the last paper, the authors made a comparative study on the relationship between clinical conditions of the development of tuberculosis and the eosinopenic response, especially, in the reference to the latter, and deduced therefrom that in very severe cases, the functions of adrenocortex fall, so that the eosinopenic response becomes weak. However, based on the eosinopenic response alone may not enough, although it may be clinically easy and convenient In the present study, the authors examined 45 tuberculosis cases, from the viewpoint of endocrinology, the eosinopenic response, uric acid creatinine ratio, and urinary 17-ketosteroid and also the Robinson-Kepler-Power test was conducted, whereby the results in their relation to the clinical conditions were analysed.
As for the urinary 17-ketosteroid, not only its total quantity of excretion for 24 hours, but also the quantity of increase in 17-ketosteroid-creatinine ratio and 17-ketosterold were investigated. As to the method of ‘storess’, ACTH method of intravenous injection was conducted.
Results were analysed by stochastic method. The results were as follows:
1) Risk of stochastic error being less than 5%, the esinopenic response in serious cases of tuberculosis did not exceed 50%.
2) In the comparison without discrimination of the level of eosinopenic response, according to different conditions of tuberculosis, on the condition that eosinopenic response is less than 50%, no remarkable difference was found between slight and moderate TB cases, however, serious cases showed low eosinopenic response. The risk of stochastic error in this case did not exceed 5%.
3) As for uric-acid creatinine ratio, stochastically accurate conclusion was not obtained
Both the eosinopenic response and uric-acid creatinine ratio are considered to be the signs of albumin-water metabolism in adrenocortex functions, however, according to these results, the former seems to be more proportional to, and responsible for the clinical conditions of tuberculosis than the latter.
4) As for urinary 17-ketosteroid, the total quantity of the excretion for 24 hours in serious TB did not exceed 5mg. Besides, Bolinger's ‘17-ketosteroid creatinine ratio’ failed to gave results in our experiments. As for quantity of increasein 17-ketosteroid, no remarkable results were obtained.
5) In the R-K-P test, two cases out of the 45 cases were of positive reaction. Incidentally the two cases were severe cases. From these results, it may be stated that water metabclism in severe TB is impaired (risk of stochastic error being less than 5%)
6) Putting together the results of various tests on he functions of adrenocortex, and considering the relationship with the clinical conditions of TB, the following conclusion is stated:
In case of severe TB, various functions of adrenocortex are impairid badly, in a number of ways.

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In the present paper, the influence of thiouracil upon the development of experimental liver cancer of rats induced by o-amido-azo-toluene was investigated histopathologically. Various organs, especially liver and thyroid of rats, that were used for the catalase measurement in the preceding paper, were stained by hematcxylin-ecsin and miciosopically examined. The results were as follows
1) Control experiments (rats were fed by o-a-t only) By 20-25 days, cell mitosis were seen irregularlly throughout the liver lobules, on the 30th day, radially arranged hyperplastic liver cells were seen around the portal areas, dispersing into the adjacent regions. Their cytoplasms had increased acidcphile nature, On 40th day, on the contrary, liver cells inclined to basophilism nature. Hyperplns'a of bile duct epithelium could not be seen until 76 days of the obser ation. On 102nd day, hypertrophic hyperplasia and cell polymorphism of liver cells became more evident, the lobular structure of liver were less distinct. On 122-128th day, relatively large ball-like adenomatous nodules of liver cells developed from the portal areas, while cells of several other portions fell into necrosis or degeneration. On 290th day, the neoplastic changes did not develope yet, in spite of the enormous hyperplasia of bile ducts epithelium. On 303rd day, the peripheral borders of the adenoma became more basophilic nature, slightly indistinct and vasculized, accompanied by neither necrosis nor degeneration. These basophilic cells showed increased polymorphisrn and in some parts rosett-shaped formations were found.
On the 321th day, these adenomas were almost transformed into hepatomas (carcinoma hepatocellulare) and large hepatoma nodules were proved in liver. Thyroid glands enlarged after 10 days of experiment, and they were histologically of parenchymatous struma or of adenoma natures. By 300 days, parenchymatous or follicular adenomas were predominant. 2) Combination of o-a-t feeding and intraperitoneal injection of methylthiouracil solution:
In this series, hyperplasia of liver cells appeared earlier than that of the control. By 23 days, basophilic liver cells increased in liver lobules. By 50 -103 days, hyperplasia of liver cels were limited to the portal branch regions and the liver cells in the central zones inclined to atrophic degeneration. On the 100th day, necrotic foci of various size appeared in liver tissues. Adenomas began to appear by 155th day. On Y00th day, adenomatous growth were faint and necrotic foci increased On 3eoth day, precancerous adenomas of basophilic nature appeared, and on 370th day, typical hepatomas associated with cholangiomas finally developed. So far as the thyroid glands concerred, from the start of the experiment, hyperplasia of follicular epithelium and decrease of colloidal mass were marked. By 100th day, some of the glands showed follicular adenomas or parenchymatous struma consisting of basophilic swollen follicular epithelium.
As the result of the experimental liver cnacer induced by o-a-t., its development was ddlayed about 63 days by the administration of thiouracil. The results were confirmed both pathohistologically and biochemically.

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The authors have recently several cases, in which gastric symptoms, such as acidismus, gastralgia, vomitus or nausea, resistance with oppressive pain at the ileocoecal region, irregular bowel movements and frequent emaciation were predominant. Symmetrical rcentgernoiogical examination of the gastro-intestinal tracts, stomach and ducdenum did not give any notable changes, however, in the small intestine, the passage of the x-ray contrastmedia was slowed down, and at the end of ileum, abnormal x-ray images, corresponding to the pressure resistance of the ileocoecal region were found. These abnormal x-ray pictures were sometimes organically consistent to the extent of ‘ileitis terminalis’, however, in some cases they were fairly variable. It is thought that the functional disorders, such as spasmus, or the increased irritability are more pronounced. These cases took chronic progress and protracted and resist against medical ti eirp es, including antibiotics therapy.
Upon the data of our 11 cases, the authors propose to name the complex of these symptoms as ‘ilex-terminal syndrome’.

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The author has reported in the last paper the improved micromethod for the quantitative analysis of dextran and caramel, which was proved to be superior to the Bloom & Wilcox's method. In the present paper, the author reports the results of the examination of the rate of recoveries of dextran and caramel in serum and urine, of the time of retention in blood and of the rate of discharge in urine, by means of the improved method.
The recoveries of dextran (alkali-digest method) and caramel (deproteinizing by 5% of trichloracetic acid) in serum and urine were 96-99% and the method was proved to be useful for the biochemical application. Examination of the excretion rate of 100ml of dextran and caramel in the normal adult human blood, revealed that the former remained over 72 hours, as Bloom and Wilcox reported, and the latter excreted within 6 hours.

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