The morphological changes of skin mast cells of rat and man under various experimental conditions were studied with electron microscope. Different mast cell changes in response to each stimulus were recognized. In most cases of them degranulation was induced. Fundamentally, cellular changes were classified in four types which were morphologically consisent with secretory mechanism of the gland cells. Secretory activity of tissue mast cells was discussed.
We have experienced a case of Acropathie ulcéromutilante. The patient is a 16 years old boy. Since ca 3-4 years ago, he has ulcer of left sole with sensory disturbance, and ulcer is not currable for the treatment. This diseas has both features of type de Thevéard and type de Giaccai in Acropathie ulcéromutilante.
A 13-year-old girl with the clinical picture of ichthyosis hystrix was reported. Some biopsy specimens of the case, investigated histologically and electron microscopically, showed a pattern characteristic of epidermolytic hyperkeratosis. The therapeutic effects of topical application of an ointment containing 0.1 % vitamin A acid were examined in the case. The effective result appeared after one month on the lesion with a marked keratolytic action clinically and histologically.
An electron microscopic study on the skins of human fetuses of 21 weeks of menstrual age revealed the following structures in some undifferentiated-type cells: (1) a certain foamy structure contained within a glycogen area consisting of clustered vesicles (Fig. 3, F). Each of these vesicles showed the profile limited not by a unit membrane, but by a less electron dense thin layer of circular form; (2) an empty structure contained within a glycogen area (Fig. 4, E); (3) a myelin figure enclosing various amounts of glycogen area (Fig. 5, My1, My2); and (4) a single membrane-limited dense body containing electron dense matrix (Fig. 4, D). These structures seemed to suspect that every one of them may represent one aspect of precursor stage of the lipid formation occuring in the undifferentiated-type cell and being closely associated with the glycogen metabolism.
Electron microscopic study on squamous cell carcinoma in burn scar revealed followings, concerning its horny cell (keratotic and parakeratotic cells) layer in cancer nests. Keratin pattern was noticeable in keratotic and parakeratotic cancer cells, but appeared to be incomplete in various degrees. Their cytoplasm contained many round structures, some of which were presumed to be derived from degenerated mitochondria or from abnormally changed intercellular spaces, but others seemed to be of a lipid nature.