We compared five topically applied corticosteroids of creams and ointments on DNA synthesis using rat skin. All preparations caused inhibition of DNA synthesis. The potency of inhibition was listed on the term of increasing order as follows: 0.1% hydrocortisone 17-butyrate, 0.12% betamethasone 17-valerate, 0.02% flumethasone pivarate, 0.05% clobetasol 17-propionate and 0.1% triamcinolone acetonide. Steroids in creams and ointments were in the same order. The studies indicated that topical application is a more potent inhibition than is intramuscular administration.
A combined antibiotic drug, Combipenix, (ampicillin and dicloxacillin) was evaluated clinically and experimentally. 1) Combipenix was administered to 30 patients with infectious skin diseases and found to be very effective in 3 cases, effective in 25 cases and ineffective in 2 cases. As a side effect, the gastric irritation was observed in 2 patients of them. 2) Organisms isolated from clinical specimens were mainly Staphylococcus aureus and S. epidermidis. 3) MIC of 21 organisms was measured and this combined antibiotic drug was confirmed to have potentiation actions on the experimental studies. Especially MDI-PC was very effective for Staphylococcus, while some strains of Staphylococcus were resistant to AB-PC. 4) On the experimental studies in rabbits, both AB-PC and MDI-PC concentrated more in the blood than in the skin. MDI-PC levels in the skin was lower than that of AB-PC. 5) Combipenix is considered to be a safe and effective drug for infectious skin diseases.