Immunopathological studies were performed on the renal tissue of 20 biopsy cases and 7 autopsy cases of lupus nephritis by means of the direct immunofluorecent method and on that of some of them by the electron-microscopic enzymelabeled antibody method. The results obtained were as follows.
1) Serological abnormality, i. c. more than 20 per cent in serum anti-DNA antibody level and less than 20 in CH50 level was seen in 88 per cent of the active lupus nephritis group (Shur’s classification) and in a few of the inactive and without group.
2) Staining patterns observed in the glomeruli of lupus nephritis by the fluorescent antibody method can be classified into three groups: linear, granular and lumpy patterns.
3) The linear, granular and lumpy patterns were observed in the majority of the inactive or without group, active group and cases expired by uremia, respectively.
4) The majority of cases displaying the granular and lumpy patterns belonged to the group showing more than 20 per cent in anti-DNA antibody level and less than 20 in CH50 level and many of cases displaying the linear pattern to the group showing less than 20 per cent in anti-DNA antibody level and more than 20 in CH50 level.
5) Localization of IgG and
β1c was almost same in the granular and lumpy patterns. No
β1c was detectable in the linear patterns.
6) The cases showing the granular pattern revealed electron-dense deposits in the glomerular basement menbrane by electron microscopy and fine granules indicating the localization of
γ-globulin within the deposits by the enzyme-labeled antibody method.
7) Fluorescence in the granular and lumpy patterns was remarkably reduced by acid treatment and the eluate was observed to contain anti-nuclear factor. Hence, it was presumed that the deposits seen in these patterns contained anti-nuclear factor-nuclear substance complex.
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