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Masaki MATSUO
Article type: Article
1969 Volume 39 Issue 1 Pages
1-10
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
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Experimental studies on the uptake of vitamin E-^<14>C in various organs and tissues were performed using normal and at 14,18 days of pregnancy female albino rats weighing 150〜200 g by means of liquid scintillation method. The uptakes of vitamin E -^<14>C were investigated on the hypophysis, adrenals, uterus, ovary, blood, liver and feces at the expected times. The uptake of hypophysis was lower than the other organs. Adrenals and ovary showed prominent uptake indicating the high affinity with vitamin E. It was found that the uptake of the uterus was high during the pregnancy showing the function as the organ for storing of vitamin E. The uptake in placenta increased with the advancement of pregnancy and the excretion of vitamin E-^<14>C into amniotic fluid was cosiderably low.
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Ichiro KUROKAWA, Toshiyuki KIMURA, Yoshiko UENO, Keiko NAGAHAMA, Tomok ...
Article type: Article
1969 Volume 39 Issue 1 Pages
11-15
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
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The influence of vitamin E on the osmotic fragility of preserved erythrocyte was investigated. The blood samples were obtained from 10 normal persons in citrate・phosphate・glucose solution. Alpha-tocopheryl-acetate was added with the concentration of 10,20 or 50 mg%. The osmotic fragility was measured by Dacie's method, once or twice a week for 3 to 4 weeks. The osmotic fragility was evidently increased in control and tocopherol groups, but it was less marked in the latter. The protective potency of the increase of osmotic fragility was the order of 50mg>20mg>10mg of tocopherol. These results show that vitamin E lessens the increase of osmotic fragility of erythrocyte in preserved blood.
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Toshikazu TABATA, Yasuyuki SHIMIZU
Article type: Article
1969 Volume 39 Issue 1 Pages
16-20
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
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In order to establish the fluorometry of tocopherols, dl-a-tocopherol acetate was saponified by potassium hydroxide with manganese dioxide. Strong fluorescence was observed on the unsaponifiable matter which showed a maximum at 385 mμ. Fractionating the fluorescent substances by thin layer chromatography, and the most conspicuous fluorescent spot was extracted with benzene from the chromatogram. The fluorescent spectra was identical with that of the original unsaponifiable matter. Linear relationship was noted between the amount of the unsaponifiable matter in benzene and the intensity of fluorescence at 385 mμ if the original dl-a-tocopherol acetate was limited under 0.3 mg per ml of the benzene. These facts suggest a possibility to determine the tocopherols fluorometrically.
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Toru ADACHI, Yukio NAKAJI, Masato ASAHINA, Toru SHIINA
Article type: Article
1969 Volume 39 Issue 1 Pages
21-25
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
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After intraperitoneal injection of riboflavin tetranicotinate (B_2-NiA) equivalent to 150 mg/kg of nicotinic acid, the amount of NAD in the mouse liver attained the maximum value at 8 hours, which was considerably higher than in the case of nicotinic acid and almost the same as that in the case of nicotinamide 150mg/kg. Larger dose of B_2-NiA caused to delay the time to the maximum amount of NAD, but the amount was rather small. However, the amount of NAD in the liver 8 hours after oral administration increased as the dose of B_2-NiA increased. The amount was more than in the case of nicotinic acid, but much less than nicotinamide. The total amount of riboflavin in the liver exhibited transient increase after intraperitoneal injection of B_2-NiA. That was attributed to increase of free riboflavin, B_2-NiA and its partial hydrolysates, and the amounts of FAD and FMN were constantly kept in the same as the normal values. In the case of oral administration of B_2-NiA, the total amount of riboflavin in the liver was not changed, and B_2-NiA and its partial hydrolysates were not demonstrated in the liver, though they were found in the large amounts in the intestinal contents.
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Nobuyasu MIZUNO, Masaru AOKI, Akira KAMADA, Noboru YATA
Article type: Article
1969 Volume 39 Issue 1 Pages
26-32
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
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The effect of vehicles on percutaneous and intradermal absorptions of pyridoxine (PIN) and pyridoxine 3,4-dioctanoate (PIN-DK) was studied in rabbit. PIN was easily absorbed from an oleyl alcohol vehicle but not from hydrophilic vehicles. Irrespective of hydrophilic or lipophilic vehicles, PIN was easily absorbed from all vehicles planted into derm of the rabbit abdomen. The blood concentration reached maximum within one hour after the plantation. It was concluded that the barrier in rabbit skin was important for the percutaneous absorption of PIN. A study of percutaneous and intradermal absorptions of PIN-DK revealed that the blood concentration increased slowly and was influenced slightly by the change of vehicles. For the percutaneous absorption of PIN-DK, the presence of barrier had little influence. PIN-DK was found to penetrate little through the skin with an in vitro experiment, but PIN, a product of PIN-DK after an enzymatic hydrolysis, did. It was considered that PIN-DK could permeate into the barrier because of its lipophilic property but could not penetrate into blood because of its poor water solubility. The rate limiting process for the percutaneous absorption of PIN-DK was concluded to be an enzymatic hydrolysis during the penetration through the skin.
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Taku TSUJIMURA, Katsuko MICHINAKA
Article type: Article
1969 Volume 39 Issue 1 Pages
33-38
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
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When thiamine standard solution in 25% KCl in 0.1N HCl is determined fluorometrically similarly to the eluate from zeolite, some KCl preparations labeled as specifially pure JIS reagent showed remarkably high blanks. However, the eluate from zeolite showed always low blanks. Calculation from the standard values corrected with high blanks led to erroneous values, but the values calculated from the blank which had been obtained by adding a drop of concentrated HCl to the thiochrome solution in isobutanol gave reasonable values. It is applicable to both ferricyanide and cyanogen bromide methods. In case of KCl・HCl solution showing high blanks, the values become higher with the increase of thiamine in the presense of NaOH, but the high blanks failed to appear in the absense of either thiamine or NaOH. The KCl preparations showing high blanks lost the blank fluorescence after heating at 600℃ for 4 hours or the KCl・HCl solution is allowed to stand at room temperature for more than ten days or is boiled for a short time. These properties were similar to the contamination of ferrcyanide in KCl preparation. Fe^<3+> had no effect on the blank fluorescence but the contamination of ferricyanide in KCl preparations with the iron content lower than the permitted limit of JIS standard gave remarkably high blanks. The tests for ferricyanide ion in KCl preparations using FeSO_4 or benzidine were not sensitive enough to detect the amount of ferricyanide supposed to be present in KCl preparations.
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Hideo NAKAYAMA, Kimitoshi NIGOTA
Article type: Article
1969 Volume 39 Issue 1 Pages
39-45
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
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A mutant strain, Escherichia coli W2-3,which required either thiamine or succinate for aerobic growth was isolated. Anaerobically the mutant grew on glucose-ammonium salts medium as well as the wild type. The amounts of thiamine required for a half-maximum growth of the mutant were observed to be at least 10 times more than that required by another thiamine-less mutants, whose metabolically blocked steps involved in thiamine biosynthesis had been determined. No experimental evidence, however, could be obtained in this mutant to suggest that the pathway to thiamine was blocked genetically. It seems likely that the reductive pathway to succinate is active under anaerobic but not under aerobic conditions and the addition of external thiamine restores the aerobic formation of succinate from α-ketoglutarate.
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Johji YAMAHARA, Hiroe KISHI, Eiichi HIRAOKA
Article type: Article
1969 Volume 39 Issue 1 Pages
46-50
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
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Deoxythiamine (DOB_1) is a new type of vitamin B_1 antagonist (Vitamins 36,326. 1967) . S-Benzoyldeoxythiamine (BDOB_1) was synthesized from DOB_1 by applying the method of benzoylation. of B_1 and its B_1 antagonistic effect was compared with that of DOB_1 on microorganisms. BDOB_ itself showed no inhibitory effect on the growth and on B_1 uptake of L. fermenti and Kl. apiculata while the coexistence of cysteine led to remarkable B_1 antagonistic effect. It was observed that BDOB_1 was changed by cysteine to DOB_1 in the medium which resulted in inhibitory effect.
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Johji YAMAHARA, Hiroe KISHI, Eiichi HIRAOKA
Article type: Article
1969 Volume 39 Issue 1 Pages
51-54
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
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Oxydeoxythiamine (oxy-DOB_1), NH_2 group of primidine moiety of DOB_1 was substituted by OH, was synthesized from DOB_1 by applying the method of oxythiamine synthesis from B_1 and its B_1 antagonistic effects on microorganisms were compared with those of DOB_1 Oxy-DOB_1 had no inhibitory effect on the growth and on B_1 uptake of L. fermenti and Kl. apiculata. From these results, it seemed Ithat NH_2 group of pyrimidine moiety was essential to work as B_1 antagonist. To make clear the importance of NH_2 group, other oxy-derivatives of B_1 antagonists, such as chloroethylthiamine and amprolium were synthesized and their effects were tested. Same results as oxy-DOB_1 were observed with oxy-chloroethylthiamine and oxy-amprolium on the growth and on B_1 uptake of the microorganisms.
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Kijuro OBARA, Shigeru ONO, Masao HATANO
Article type: Article
1969 Volume 39 Issue 1 Pages
55-58
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
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The changes of phospholipid metabolism in rat liver were investigated when riboflavin or its butylester was administered orally. The amount of liver phospholipid did not change in either free or butylester of riboflavins administration. The incorporation rate of ^<32>P into phospholipid fraction was found to be maximum after 24 hrs. in riboflavin administration but it was 48 hrs. in butyrate. On the other hand, specific activity of phospholipid fraction of both groups were maximum at 48 hours. Phosphatidylcholine and phosphatidylethanolamine fraction. showed high radioactivities and these tendencies were found remarkably in the groups of butyl ester. Phosphatidylcholine concenrtation was found to be increased by administration of large amount of butylester. From these results, it suggests that the administration of riboflavin induces the increase of turnover of phospholipid in the liver and the phosphorylation process of phospholipid is coupled with that of riboflavin and elevetion of specific activity of phosphatidylcholine fraction play an important role for the regulation of fatty acid metabolism in the organs, such as liver and blood vessels.
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1969 Volume 39 Issue 1 Pages
59-
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1969 Volume 39 Issue 1 Pages
59-60
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
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[in Japanese], [in Japanese]
Article type: Article
1969 Volume 39 Issue 1 Pages
60-
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1969 Volume 39 Issue 1 Pages
60-
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1969 Volume 39 Issue 1 Pages
60-61
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1969 Volume 39 Issue 1 Pages
61-
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
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[in Japanese]
Article type: Article
1969 Volume 39 Issue 1 Pages
61-62
Published: January 25, 1969
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[in Japanese]
Article type: Article
1969 Volume 39 Issue 1 Pages
62-
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
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[in Japanese], [in Japanese]
Article type: Article
1969 Volume 39 Issue 1 Pages
62-63
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
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[in Japanese], [in Japanese]
Article type: Article
1969 Volume 39 Issue 1 Pages
63-64
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1969 Volume 39 Issue 1 Pages
64-
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
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[in Japanese]
Article type: Article
1969 Volume 39 Issue 1 Pages
64-65
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1969 Volume 39 Issue 1 Pages
65-
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
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[in Japanese], [in Japanese]
Article type: Article
1969 Volume 39 Issue 1 Pages
65-66
Published: January 25, 1969
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1969 Volume 39 Issue 1 Pages
66-
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
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[in Japanese], [in Japanese]
Article type: Article
1969 Volume 39 Issue 1 Pages
66-
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
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[in Japanese], [in Japanese]
Article type: Article
1969 Volume 39 Issue 1 Pages
67-
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1969 Volume 39 Issue 1 Pages
67-68
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
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[in Japanese], [in Japanese]
Article type: Article
1969 Volume 39 Issue 1 Pages
68-69
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
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[in Japanese]
Article type: Article
1969 Volume 39 Issue 1 Pages
69-
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1969 Volume 39 Issue 1 Pages
69-70
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
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[in Japanese], [in Japanese]
Article type: Article
1969 Volume 39 Issue 1 Pages
71-
Published: January 25, 1969
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[in Japanese]
Article type: Article
1969 Volume 39 Issue 1 Pages
71-72
Published: January 25, 1969
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[in Japanese]
Article type: Article
1969 Volume 39 Issue 1 Pages
72-73
Published: January 25, 1969
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[in Japanese]
Article type: Article
1969 Volume 39 Issue 1 Pages
73-74
Published: January 25, 1969
Released on J-STAGE: February 17, 2018
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