VITAMINS

Online ISSN : 2424-080X
Print ISSN : 0006-386X

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Volume 61, Issue 10

Displaying 1-26 of 26 articles from this issue

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Changes of Enzyme Activities on the Choline Metabolism in the Rat Liver

Haruhito TSUGE

Article type: Article
1987 Volume 61 Issue 10 Pages 471-483
Published: October 25, 1987
Released on J-STAGE: March 16, 2018

DOIhttps://doi.org/10.20632/vso.61.10_471

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Liver is the most important site for choline metabolism and possesses all enzymes relating to three different metabolic pathways. In order to find the metabolic change of hepatic choline-flux concerning sex differences, senility and administration of hepatotoxine, two enzyme activities, one is choline dehydrogenase (EC 1.1.99.1), the initiating enzyme for oxidation pathway, and the other is choline kinase (EC 2.7.1.32) for Kennedy pathway, were measured with rat liver. A close correlation between choline dehydrogenase activity and body weight as well as liver weight was obtained on the normal feeding rats aging from 25 day-old to 12 month-old, totaling 132, independent of sex. Choline dehydrogenase activity of senile rat was significantly depressed. Upon ethionine feeding, choline dehydrogenase activity was considerably depressed. On the other hand, choline kinase activity was activated twice, presumably, for the metabolic compensation.

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Purification and Properties of Intracellular Thiaminase II of Bacillus Aneurinolyticus

Toshiyuki NAKATSUKA, Kiroku SUZUKI, Yoshihisa NAKANO, Shozaburo KITAOK ...

Article type: Article
1987 Volume 61 Issue 10 Pages 485-492
Published: October 25, 1987
Released on J-STAGE: March 16, 2018

DOIhttps://doi.org/10.20632/vso.61.10_485

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Thiaminase II (Thiamin hydrolase) of Bacillus aneurinolyticus was purified to homogeneity. The enzyme activity was spectrophotometrically determined by measuring the rate of decrease in absorbance at 318 nm. The optimum pH and temperature were 9.4 and 65℃, respectively. Apparent activation energy was calculated to be 16.1 kcal/M from Arrhenius plots, and the Km value was 8.4 μM. Some sulfhydryl reagents enhanced the enzyme activity by 180-300%, while EDTA had no effect. Zn^lt:2+> among metal ions tested strongly inhibited the enzyme activity.

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Effect of Dietary N^1-Methylnicotinamide or Trigonelline on the Growth and the Niacin Metabolism in Weanling Rats

Katsumi SHIBATA, Hiroshi TAGUCHI

Article type: Article
1987 Volume 61 Issue 10 Pages 493-499
Published: October 25, 1987
Released on J-STAGE: March 16, 2018

DOIhttps://doi.org/10.20632/vso.61.10_493

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The conversion of N^1-methylnicotinamide (MNA) and trigonelline (TRG) to niacin and their toxicity in rats were investigated. The conversion of MNA into nicotinamide in rats did not seem to occur since the niacin effect was not observed when MNA was administered with tryptophan-imbalanced-niacin-free diet to weanling rats. When a large amount of MNA or TRG was administered to weanling rats, no growth retardation was observed. All of the TRG taken was excreted unchanged into urine. On the other hand, 10% of MNA taken was metabolized to N^1-methyl-2-pyridone-5-carboxamide and 70% of MNA was excreted unchanged. Even when a large amount of TRG or MNA was administered to rats, urinary excretion of niacin did not increase. From these results, it is concluded that the conversion of MNA and TRG into niacin does not occur and MNA and TRG are inert and very safe compounds in rats.

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Vitamin B_1 Activity in Lactobacillus Fermenti of Vitamin B_1-like Substances Existing in Canned Mackerel and Vitamin B_1 Content of Canned Fish, Pork, Beef and Vegetables

Tomio ICHIKAWA, Akiko TSUDA, Masako SAKURADA, Reiji YOSHIKAWA

Article type: Article
1987 Volume 61 Issue 10 Pages 501-504
Published: October 25, 1987
Released on J-STAGE: March 16, 2018

DOIhttps://doi.org/10.20632/vso.61.10_501

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Vitamin B_1 content of canned fish, pork, beef and vegetable was determined by three methods using various solvents and oxidation reagents. Vitamin B_1 activity of vitamin B_1-like substance existing in canned mackerel was investigated using Lactobacillus fermenti. 1) The method using K_3Fe(CN)_6-oxidation and benzene : n-butanol extraction seems to give true vitamin B_1 values of canned foods examined but does not by K_3Fe(CN)_6-oxidation and isobutanol extraction. 2) Vitamin B_1-like substances existing in canned mackerel exhibited no vitamin B_1 activity in L. fermenti.

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[title in Japanese]

Article type: Appendix
1987 Volume 61 Issue 10 Pages 505-508
Published: October 25, 1987
Released on J-STAGE: March 16, 2018

DOIhttps://doi.org/10.20632/vso.61.10_505

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1987 Volume 61 Issue 10 Pages 509-
Published: October 25, 1987
Released on J-STAGE: March 16, 2018

DOIhttps://doi.org/10.20632/vso.61.10_509_1

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1987 Volume 61 Issue 10 Pages 509-510
Published: October 25, 1987
Released on J-STAGE: March 16, 2018

DOIhttps://doi.org/10.20632/vso.61.10_509_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1987 Volume 61 Issue 10 Pages 510-
Published: October 25, 1987
Released on J-STAGE: March 16, 2018

DOIhttps://doi.org/10.20632/vso.61.10_510

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

Article type: Article
1987 Volume 61 Issue 10 Pages 511-
Published: October 25, 1987
Released on J-STAGE: March 16, 2018

DOIhttps://doi.org/10.20632/vso.61.10_511_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

Article type: Article
1987 Volume 61 Issue 10 Pages 511-
Published: October 25, 1987
Released on J-STAGE: March 16, 2018

DOIhttps://doi.org/10.20632/vso.61.10_511_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1987 Volume 61 Issue 10 Pages 511-512
Published: October 25, 1987
Released on J-STAGE: March 16, 2018

DOIhttps://doi.org/10.20632/vso.61.10_511_3

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1987 Volume 61 Issue 10 Pages 512-
Published: October 25, 1987
Released on J-STAGE: March 16, 2018

DOIhttps://doi.org/10.20632/vso.61.10_512

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[title in Japanese]

[in Japanese]

Article type: Article
1987 Volume 61 Issue 10 Pages 513-
Published: October 25, 1987
Released on J-STAGE: March 16, 2018

DOIhttps://doi.org/10.20632/vso.61.10_513_1

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[title in Japanese]

[in Japanese]

Article type: Article
1987 Volume 61 Issue 10 Pages 513-514
Published: October 25, 1987
Released on J-STAGE: March 16, 2018

DOIhttps://doi.org/10.20632/vso.61.10_513_2

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1987 Volume 61 Issue 10 Pages 515-516
Published: October 25, 1987
Released on J-STAGE: March 16, 2018

DOIhttps://doi.org/10.20632/vso.61.10_515

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1987 Volume 61 Issue 10 Pages 516-
Published: October 25, 1987
Released on J-STAGE: March 16, 2018

DOIhttps://doi.org/10.20632/vso.61.10_516_1

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1987 Volume 61 Issue 10 Pages 516-517
Published: October 25, 1987
Released on J-STAGE: March 16, 2018

DOIhttps://doi.org/10.20632/vso.61.10_516_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1987 Volume 61 Issue 10 Pages 517-518
Published: October 25, 1987
Released on J-STAGE: March 16, 2018

DOIhttps://doi.org/10.20632/vso.61.10_517

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1987 Volume 61 Issue 10 Pages 519-520
Published: October 25, 1987
Released on J-STAGE: March 16, 2018

DOIhttps://doi.org/10.20632/vso.61.10_519

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1987 Volume 61 Issue 10 Pages 520-
Published: October 25, 1987
Released on J-STAGE: March 16, 2018

DOIhttps://doi.org/10.20632/vso.61.10_520_1

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[title in Japanese]

[in Japanese]

Article type: Article
1987 Volume 61 Issue 10 Pages 520-521
Published: October 25, 1987
Released on J-STAGE: March 16, 2018

DOIhttps://doi.org/10.20632/vso.61.10_520_2

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[title in Japanese]

[in Japanese]

Article type: Article
1987 Volume 61 Issue 10 Pages 523-524
Published: October 25, 1987
Released on J-STAGE: March 16, 2018

DOIhttps://doi.org/10.20632/vso.61.10_523

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[title in Japanese]

[in Japanese]

Article type: Article
1987 Volume 61 Issue 10 Pages 524-526
Published: October 25, 1987
Released on J-STAGE: March 16, 2018

DOIhttps://doi.org/10.20632/vso.61.10_524

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[title in Japanese]

[in Japanese]

Article type: Article
1987 Volume 61 Issue 10 Pages 526-527
Published: October 25, 1987
Released on J-STAGE: March 16, 2018

DOIhttps://doi.org/10.20632/vso.61.10_526

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1987 Volume 61 Issue 10 Pages 527-528
Published: October 25, 1987
Released on J-STAGE: March 16, 2018

DOIhttps://doi.org/10.20632/vso.61.10_527

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1987 Volume 61 Issue 10 Pages 529-530
Published: October 25, 1987
Released on J-STAGE: March 16, 2018

DOIhttps://doi.org/10.20632/vso.61.10_529

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