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Volume 50, Issue 3
Displaying 1-35 of 35 articles from this issue
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Nobuyasu MIZUNO, Michiyo FUJIMOTO, Akira KAMADAArticle type: Article
1976 Volume 50 Issue 3 Pages 85-91
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESS3,α^4-Di-O-dioctanoyl pyridoxine was transformed into α^4-N-octanoyl pyridoxamine by reacting with ammonia at 60℃ for 3 hours. This conversion also occurred with α^4,α^5-Di-O-dioctanoyl pyridoxine and 5-O-octanoyl pyridoxine at the same condition. α^4-N-Octanoyl pyridoxamine was also synthesized by reaction of pyridoxamine HCl with caprylyl chloride. α^4-N-octanoyl pyridoxamine obtained by the two methods agreed in mp, IR and thin layer chromatography. α^4-N-acetyl pyridoxamine and α^4-N-lauroyl pyridoxamime were synthesized in the same manner. Solubilities of α^4-O-octanoyl pyridoxine and α^4-N-octanoyl pyridoxamine in organic solvents and their spectronic properties were measured. Effect of boric acid on the colouration of pyridoxamine derivatives with a 2,6 -dibro-moquinonechloroimide was studied. Hydrolysis of α^4-N-octanoyl pyridoxa mine by acid, alkali and enzymes was also investigated.View full abstractDownload PDF (666K) -
Sei-ichiro IKEDA, Hitoshi HARA, Osamu SUGIMOTO, Saburo FUKUIArticle type: Article
1976 Volume 50 Issue 3 Pages 93-96
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSA derivative of pyridoxal 5'-phosphate (PALP) bund to Sepharose 4B through the OH-group at position 3 of PALP was prepared by reacting PALP with bromoacetamidohexyl-Sepharose at pH 6.5. A Sepharose-bound tryptophan-PALP complex was also derived from the Sepharose-bound PALP by reducing the Schiff base between Sepharose-bound PALP and tryptophan with NaBH_4. These Sepharose-bound derivatives of PALP were found to be very useful as bio-specific adsorbents for apo-tryptophanase : Apo-tryptophanase adsorbed on a column of Sepharose -bound PALP was eluted specifically with a mixture of potassium phosphate buffer (0.5M, pH 7.0) and PALP (1mM), thus being purified 8〜10 times; much more specific elution of apotryptophanase adsorbed on a column of Sepharose-bound tryptophan-PALP comp1ex was observed. The apoprotein was eluted only with a mixture of PALP, tryptophan and potassium phosphate buffer, being much more efficiently purified by the affinity chromatography on the column.View full abstractDownload PDF (409K) -
Takahiko TSUJI, Ryo-hei YAMADAArticle type: Article
1976 Volume 50 Issue 3 Pages 97-101
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSThe transport of ^3H-pyridoxal (^3H-PAL) across the intestinal wall was studied with the everted sacs of rat small intestine, mainly at an initial ^3H-PAL concentration of 0.3μM on the mucosal side. The serosal concentration of labelled compounds continuously increased, but did not reach the mucosal level during 4-hour incubation at 37℃, and the time course apparently subjected to first-order kinetics during the first 2 hours of incubation. Addition of 300μM 4-deoxypyridoxine or 10-fold increase of initial mucosal ^3H-PAL concentration did not significantly affect the time course of the ratio of serosal to mucosal concentration, but affected the extent of ^3H-PAL uptake by the tissue. The transport at 0℃ was much slower than that at 37℃ and apparently subjected to first order kinetics for 4 hours. Comparison of the rate constants with those of pyridoxine showed that PAL was transported faster than pyridoxine either at 37℃ or 0℃. Analysis of the serosal ^3H-compounds after 2-hour incubation with 0.3 or 3μM ^3H-PAL by column chromatography on Dowex 50W indicated that almost of them was ^3H-PAL.View full abstractDownload PDF (462K) -
Takahiko TSUJI, Ryo-hei YAMADAArticle type: Article
1976 Volume 50 Issue 3 Pages 103-108
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSThe transport of ^3H-pyridoxamine (^3H-PAM) from the mucosal to serosal side of everted sacs was examined mainly at an initial mucosal ^3H-PAM concentration of 0.3μM, at 37℃ for 4 hours. The serosal concentration of ^3H-compounds never reached the mucosal level, and the time course of the ratio of serosal to mucosal concentration was not significantly affected by the addition of 300μM 4-deoxypyridoxine (DOPIN) or 10-fold increase of initial ^3H-PAM concentration. The transport was remarkably depressed by lowering the temperature to 0℃. Comparison of the three forms of vitamin B_6 with respect to the rate of transport gave the following order : pyridoxal (PAL)>PAM>pyridoxine (PIN), which was different from the order of uptake rate by the tissue. The main component of the serosal ^3H-compounds after 2-hour transport was ^3H-PAM, but the presence of ^3H-PAL was also significant. For comparison the serosal fluid after incubation of everted sacs with 0.3μM ^3H-PIN was examined, and was found to contain much more ^3H-PAL than the case of ^3H-PAM transport. Its relative amount diminished by the addition of phosphate buffer or DOPIN, or by 10-fold increase of the initial mucosal concentration of ^3H-PIN.View full abstractDownload PDF (559K) -
[in Japanese], [in Japanese]Article type: Article
1976 Volume 50 Issue 3 Pages 109-
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (150K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1976 Volume 50 Issue 3 Pages 109-
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (150K) -
[in Japanese], [in Japanese]Article type: Article
1976 Volume 50 Issue 3 Pages 109-110
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (292K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1976 Volume 50 Issue 3 Pages 110-
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (191K) -
[in Japanese], E.C. Ellison, G.R. Mizuno, J.R. ChipaultArticle type: Article
1976 Volume 50 Issue 3 Pages 110-
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (191K) -
[in Japanese], [in Japanese]Article type: Article
1976 Volume 50 Issue 3 Pages 110-
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (191K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1976 Volume 50 Issue 3 Pages 111-
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (108K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1976 Volume 50 Issue 3 Pages 111-
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (108K) -
[in Japanese]Article type: Article
1976 Volume 50 Issue 3 Pages 113-
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (169K) -
[in Japanese]Article type: Article
1976 Volume 50 Issue 3 Pages 113-114
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (291K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1976 Volume 50 Issue 3 Pages 114-
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (178K) -
[in Japanese]Article type: Article
1976 Volume 50 Issue 3 Pages 115-
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (139K) -
[in Japanese]Article type: Article
1976 Volume 50 Issue 3 Pages 115-116
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (287K) -
[in Japanese]Article type: Article
1976 Volume 50 Issue 3 Pages 116-118
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (455K) -
[in Japanese]Article type: Article
1976 Volume 50 Issue 3 Pages 118-
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (193K) -
[in Japanese]Article type: Article
1976 Volume 50 Issue 3 Pages 118-119
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (319K) -
[in Japanese]Article type: Article
1976 Volume 50 Issue 3 Pages 119-121
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (426K) -
[in Japanese]Article type: Article
1976 Volume 50 Issue 3 Pages 121-122
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (276K) -
[in Japanese]Article type: Article
1976 Volume 50 Issue 3 Pages 123-124
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (320K) -
[in Japanese], [in Japanese]Article type: Article
1976 Volume 50 Issue 3 Pages 125-
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (144K) -
[in Japanese], [in Japanese]Article type: Article
1976 Volume 50 Issue 3 Pages 125-126
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (285K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1976 Volume 50 Issue 3 Pages 126-127
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (332K) -
[in Japanese]Article type: Article
1976 Volume 50 Issue 3 Pages 127-128
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (328K) -
[in Japanese]Article type: Article
1976 Volume 50 Issue 3 Pages 128-129
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (326K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1976 Volume 50 Issue 3 Pages 129-130
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (331K) -
[in Japanese]Article type: Article
1976 Volume 50 Issue 3 Pages 130-
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (190K) -
[in Japanese], [in Japanese]Article type: Article
1976 Volume 50 Issue 3 Pages 130-131
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (323K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1976 Volume 50 Issue 3 Pages 131-
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (182K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
1976 Volume 50 Issue 3 Pages 132-
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (189K) -
[in Japanese]Article type: Article
1976 Volume 50 Issue 3 Pages 132-133
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (260K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1976 Volume 50 Issue 3 Pages 133-
Published: March 25, 1976
Released on J-STAGE: March 06, 2018
JOURNAL FREE ACCESSDownload PDF (117K)
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