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Toshihiro SHIODA, Takashi KAWASAKI
Article type: Article
1992Volume 66Issue 10 Pages
569-580
Published: October 25, 1992
Released on J-STAGE: March 17, 2018
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Cytosolic adenylate kinase (AK1; [EC 2.7.4.3]) synthesizes thiamin triphosphate (TTP) both in vitro and in vivo. AK 1 synthesizes TTP by a reaction: TDP + ADP ⇄ TTP + AMP in addition to its main reaction: 2ADP ⇄ ATP + AMP. No TTP-synthesizing enzymes other than AK 1 were detected in the skeletal muscle. TDP is assumed to bind to the less-specific nucleotide binding site (ATP binding site) of AK 1 as a substrate, while ADP binds to the highly-specific site (AMP binding site), which results in formation of TTP by phosphate transfer from ADP.
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Shuzi YAGI, Keiichi UNO, Shiro SAITO, Hiroyuki NODA
Article type: Article
1992Volume 66Issue 10 Pages
581-589
Published: October 25, 1992
Released on J-STAGE: March 17, 2018
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Since geometric isomers of retinol are not easily obtained, the individual determination of vitamin A groups in foods by high performance liquid chromatography has not yet been performed. In the present experiment, attempts were made to measure each isomer. The following procedure was found satisfactory for this determination. All-trans-retinal was transformed into a mixture of retinol cis-trans isomers by irradiation with visible light and reduction. The mixture was chromatographed on a silica gel column with authentic specimens (13-cis and all-tans forms). The concentration of each isomer was evaluated on the basis of the above results and the factors were confirmed in this work. Consequently, the mixture represents a retinol isomer standard. These factors were effective for isomer standardization by controlling the adsorptive activity of the silica gel, even when different columns are employed.
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Katsumi SHIBATA, Michiko ONODERA
Article type: Article
1992Volume 66Issue 10 Pages
591-596
Published: October 25, 1992
Released on J-STAGE: March 17, 2018
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We investigated the effects of 3-acetylpyridine, pyridine-3-aldehyde, pyridine-3-methanol, and β-picoline on the growth of rats, food intake, and the urinary excretion of nicotinamide, N^1-methylnicotinamide (MNA), N^1-methyl-2-pyridone-5-carboxamide (2-Py) and N^1-methyl-4-pyridone-3-carboxamide (4-Py). all the derivatives caused the significant increase in the urinary excretion of nicotinamide, MNA, 2-Py, and 4-Py. this result suggests that nicotinamide is biosynthesized via nicotinic acid-NAD pathway from the above derivatives. But, the administration of 3-acetylpyridine caused convulsion of the limbs as well as the much decrease in body weight and food intake. From these results, 3-acetylpyridine seemed likely to possess antagonistic action aganist niacin, rather than niacin activity, while, pyridine-3-aldehyde, pyridine-3-methanol, and β-picoline possessed niacin activity but not niacin antagonistic action.
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Satoshi WATANABE, Saju KAWAUCHl
Article type: Article
1992Volume 66Issue 10 Pages
597-600
Published: October 25, 1992
Released on J-STAGE: March 17, 2018
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Thiobarbituric acid-reactive substance (TBA-RS) was formed in the reaction of 2,4-hexadienal and t-butyl hydroperoxide (t-BuOOH) by heating at 95℃. The suitable amount of t-BuOOH was determined from the calibration curve of the TBA-RS formation in the reaction mixture. When riboflavin was added in this reaction mixture, TBA-RS formation was reduced proportionally with the amount of riboflavin. The riboflavin content in the reaction mixture measured by the fluorescence method was decreased with the addition of t-BuOOH. We considered that the inhibitory effect of riboflavin on the TBA-RS formation is due to the direct reaction with t-BuOOH and riboflavin degrades during the reaction.
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1992Volume 66Issue 10 Pages
601-
Published: October 25, 1992
Released on J-STAGE: March 17, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1992Volume 66Issue 10 Pages
601-602
Published: October 25, 1992
Released on J-STAGE: March 17, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1992Volume 66Issue 10 Pages
602-603
Published: October 25, 1992
Released on J-STAGE: March 17, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1992Volume 66Issue 10 Pages
603-604
Published: October 25, 1992
Released on J-STAGE: March 17, 2018
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1992Volume 66Issue 10 Pages
604-
Published: October 25, 1992
Released on J-STAGE: March 17, 2018
JOURNAL
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[in Japanese], [in Japanese]
Article type: Article
1992Volume 66Issue 10 Pages
604-605
Published: October 25, 1992
Released on J-STAGE: March 17, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...
Article type: Article
1992Volume 66Issue 10 Pages
605-606
Published: October 25, 1992
Released on J-STAGE: March 17, 2018
JOURNAL
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[in Japanese]
Article type: Article
1992Volume 66Issue 10 Pages
607-608
Published: October 25, 1992
Released on J-STAGE: March 17, 2018
JOURNAL
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1992Volume 66Issue 10 Pages
608-
Published: October 25, 1992
Released on J-STAGE: March 17, 2018
JOURNAL
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[in Japanese]
Article type: Article
1992Volume 66Issue 10 Pages
609-611
Published: October 25, 1992
Released on J-STAGE: March 17, 2018
JOURNAL
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[in Japanese]
Article type: Article
1992Volume 66Issue 10 Pages
611-613
Published: October 25, 1992
Released on J-STAGE: March 17, 2018
JOURNAL
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[in Japanese]
Article type: Article
1992Volume 66Issue 10 Pages
613-
Published: October 25, 1992
Released on J-STAGE: March 17, 2018
JOURNAL
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