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Volume 66, Issue 8
Displaying 1-19 of 19 articles from this issue
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Yasuo KITAGAwA, Masaaki ONO, Foon JENArticle type: Article
1992 Volume 66 Issue 8 Pages 431-436
Published: August 25, 1992
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSAscorbic acid is a cofactor of hydroxylation of proline and lysine in procollagen. It also stabilizes procollagen mRNAs, stimulates secretion and processing of procollagens. By stimulating bundle formation of type I collagen like this, ascorbic acid supports cultured fibroblasts to form tissue-like structure in vitro. Such activities of ascorbic acid were known only for collagens of connective tissues, but we here show that ascorbic acid phosphate (Asc-P) stimulates synthesis of basement membrane collagen (type IV) as well. Such effect of Asc-P on 3T3-L1 fibroblasts accelerated the differentiation of the cells into adipocytes.View full abstractDownload PDF (1004K) -
Minoru YANAI, Toshihiro OHMORI, Tsuyoshi NAKAMURA, Daiji YOSHIHARA, Ma ...Article type: Article
1992 Volume 66 Issue 8 Pages 437-446
Published: August 25, 1992
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSUndernourished and immunosuppressive state was produced by administration of 15-25 mg/kg of 5-Fu, an anticancer drug, seven times every other day, in SD rats. After the administration, decrease in leukocytes, weight gain and the food efficiency ratio (FER), in addition to suppression of delayed-type hypersensitivity (DTH), were markedly documented. Such impaired states with 20 mg/kg 5-Fu administration were predominant as compared with those in the rats of pair fed group without of 5-Fu. In the rats, who took the diet with either 15 or 40 ppm of retinyl palmitate or β-carotene, restoration of impaired weight gain, FER and DTH was observed, while no effects was documented on leukocyte numbers.View full abstractDownload PDF (1000K) -
Takashi UJIIE, Aiko TANAKA, Ayumi KNDO, Reiko HIRE, Tetsushige TAKEYAM ...Article type: Article
1992 Volume 66 Issue 8 Pages 447-450
Published: August 25, 1992
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSA rapid and reproducible HPLC method, after saponification and ethyl acetate-hexane extraction, was examined for the determination of tochpherols in some kinds of algae. Different values were obtained between dried purple laver (Hoshi-nori) and toasted purple laver (Yaki-nori), while the recoveries of tocopherol added to them were more than 90%. The different values between both samples were found to be due to inadequate extraction. Complete and consistent extraction of tocopherols in different kinds of samples was achieved by adding adequate amount of water and then heating at 100℃ for 5 minutes before saponification. Procedure of the maceration and heating was sufficient for complete extraction from dried and toasted purple lavers (Amanori), dried green laver (Aonori) and dried sea lettuce (Aosa). Overall recoveries of externally added tocopherol were 84.5% and 89.4%, respectively, for dried and toasted purple lavers.View full abstractDownload PDF (411K) -
Kazuhiro YAMADA, Masahiro WADA, Tetsunori KAWATA, Tadahiro TADOKORO, A ...Article type: Article
1992 Volume 66 Issue 8 Pages 451-455
Published: August 25, 1992
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSAn assay method for methionine adenosyltransferase (MAT) activity using high performance liquid chromatography (HPLC) was newly developed. The MAT reaction was carried out in the presence of K^+, Mg^<2+>, DTT and stopped by adding ethanol to a final concentration of 50% (v/v). S-adenosylmethionine (SAM) produced was separated and quantified by HPLC. The conditions of HPLC for separation of SAM were as follows: column, Partisil-10 SCX; mobile phase, 0.22M CH_3COONa pH 4.0; detection wave-length, 254nm. This assay method was used for determination of the MAT activity of rat's tissue, fed on vitamin B_<12>-deprived diet for 90 days. Hepatic MAT activity of B_<12>-deficient rat decreased to 1/4 of that of the B_<12>-supplemented rat. When DMSO was added to the reaction mixture, the enzyme activity of B_<12>-deficient rat decreased to 70% of that of B_<12>-supplemented rat.View full abstractDownload PDF (490K) -
Toshio OKANO, Tamiko KIMURA, Naoko TSUGAWA, Yoshitaka OKAMURA, Yukiko ...Article type: Article
1992 Volume 66 Issue 8 Pages 457-468
Published: August 25, 1992
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSEffect of dietary calcium intake on bone mineral contents, density and strength in vitamin D-replete rats was investigated. Vitamin D-deficient rats were fed ad libitum a diet containing various concentrations (0.22, 0.44, 0.88 ad 1.20%) of calcium adjusted with L-calcium lactate or calcium hydrogenphosphate under oral supplementation of physiological dose (10 IU/day/rat) of vitamin D_3 for 28 days. During and after feeding, concentrations of calcium, phosphorus, 25-hydroxyvitamin D_3 and alkaline phosphatase activity in plasma were measured. Futhermore, femoral bone ash weight, calcium and phosphorus contents, mineral density and strength were also measured. Despite of the differences of calcium contents and sources in diets, all groups showed a prompt and steady recovery from calcium and vitamin D malnutritions and there was no significant difference among all groups in the increases of body weight and plasma calcium level and the decrease of plasma alkaline phosphatase activity. In contrast, the groups fed diets containing L-calcium lactate showed a significant relationship between bone ash weights in femurs and calcium contents in diets, while no significant relationship was found on mineral density and strength in the same tissue. On the other hand, the groups fed diets containing hydrogenphosphate showed no significant relationship on the above bone parameters. When the groups fed the diets containing equal amounts of calcium from L-calcium lactate or calcium hydrogenphosphate were compared with each other, there was no significant difference between bioavailabilities of L-calcium lactate and calcium hydrogenphosphate although hydrogenphosphate seemed to be slightly more effective than L-calcium lactate in increasing mineral density (ΣGS/D) and strength (Torque energy). These results suggest that dietary calcium intake may affect bone growth even in vitamin D-sufficient status in rats.View full abstractDownload PDF (1150K) -
Kazuto YASUDA, Toshikazu SHIMADAArticle type: Article
1992 Volume 66 Issue 8 Pages 469-475
Published: August 25, 1992
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSIn order to compare the bioavailabilities of various forms of commercial vitamin B_6 (B_6) preparation, the changes of blood total B_6, pyridoxal 5'-phosphate (PLP) level and urinary excretion of 4-pyridoxic acid (PIC) were studied after oral administration of ordinary dose for these preparations in ten healthy male adults. The T_<max.> of total B_6 and PLP level after administration of calcium pyridoxal 5'-phosphate (PLP-Ca) was the shortest one, and also the C_<max.> of those were the highest. But the ΔAUC (24 hours) for pyridoxine hydrochloride (PN-HCl) was the largest among these three preparations. The 3 hours urinary PIC excretion ratio for PLP-Ca was the highest, and no significant differences in 24 hours excretion ratio were found among them. The major component of serum B_6 at the point of C_<max.> for PLP-Ca was pyridoxal (PL). The blood total B_6 level at the point of C_<max.>, ΔAUC and also 3 hours PIC excretion ratio showed the largest CVs in the PLP enteric coating tablet (PLP_<ent.>) group. consequently it was considered that the absorption of PLP_<ent.> was poorest in reproducibility. The ascending tendencies of baseline levels of total B_6 in serum and erythrocyte were found to be prominent in PLP-Ca group.View full abstractDownload PDF (628K) -
[in Japanese], [in Japanese]Article type: Article
1992 Volume 66 Issue 8 Pages 477-478
Published: August 25, 1992
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSDownload PDF (299K) -
[in Japanese]Article type: Article
1992 Volume 66 Issue 8 Pages 478-
Published: August 25, 1992
Released on J-STAGE: March 30, 2018
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[in Japanese], [in Japanese], [in Japanese]Article type: Article
1992 Volume 66 Issue 8 Pages 478-479
Published: August 25, 1992
Released on J-STAGE: March 30, 2018
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[in Japanese], [in Japanese], [in Japanese]Article type: Article
1992 Volume 66 Issue 8 Pages 479-480
Published: August 25, 1992
Released on J-STAGE: March 30, 2018
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[in Japanese]Article type: Article
1992 Volume 66 Issue 8 Pages 480-481
Published: August 25, 1992
Released on J-STAGE: March 30, 2018
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1992 Volume 66 Issue 8 Pages 481-482
Published: August 25, 1992
Released on J-STAGE: March 30, 2018
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[in Japanese]Article type: Article
1992 Volume 66 Issue 8 Pages 482-483
Published: August 25, 1992
Released on J-STAGE: March 30, 2018
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[in Japanese]Article type: Article
1992 Volume 66 Issue 8 Pages 483-484
Published: August 25, 1992
Released on J-STAGE: March 30, 2018
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[in Japanese], [in Japanese]Article type: Article
1992 Volume 66 Issue 8 Pages 484-
Published: August 25, 1992
Released on J-STAGE: March 30, 2018
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[in Japanese]Article type: Article
1992 Volume 66 Issue 8 Pages 485-487
Published: August 25, 1992
Released on J-STAGE: March 30, 2018
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[in Japanese]Article type: Article
1992 Volume 66 Issue 8 Pages 487-489
Published: August 25, 1992
Released on J-STAGE: March 30, 2018
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[in Japanese], [in Japanese]Article type: Article
1992 Volume 66 Issue 8 Pages 489-492
Published: August 25, 1992
Released on J-STAGE: March 30, 2018
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[in Japanese]Article type: Article
1992 Volume 66 Issue 8 Pages 492-
Published: August 25, 1992
Released on J-STAGE: March 30, 2018
JOURNAL OPEN ACCESSDownload PDF (161K)
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