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Volume 55, Issue 1
Displaying 1-30 of 30 articles from this issue
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Kazuyoshi SATOArticle type: Article
1981 Volume 55 Issue 1 Pages 1-12
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
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Kimiko WATANABE, Asako MORISHIGE, Koichiro ISOI, Ryoji HAYASHIArticle type: Article
1981 Volume 55 Issue 1 Pages 13-22
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
JOURNAL FREE ACCESSA mutant strain of Escherichia coli. D-30, requiring the pyrimidine moiety of thiamine (OMP) for both aerobic and anaerobic growth in a minimal medium supplemented with the thiazole moiety of thiamine (Th) was isolated from mutant 26-43, requiring Th. The requirement for OMP could be replaced by methionine and lysine, but not by succinate. The mutant could not grow in the minimal medium supplemented with 1 nM OMP, but the rapid growth was observed by the addition of methionine. The growth in the minimal medium Supplemented with methionine and lysine was strongly inhibited by adenosine. The growth of mutant 26-43 in the minimal medium supplemented with Th was also considerably inhibited by adenosine. This inhibition was restored by the addition of both methionine and lysine. A mutant strain, 6-60, isolated from a Th-requiring mutant (26-43), required OMP and adenosine for growth. The requirement for OMP in mutant 6-60 was substituted by methionine and lysine as found in mutant D-30. The formation of OMP by cell suspension of mutant D-30 was shown to be dependent on the presence of methioninc. This formation by cell suspensions of mutants D-30, 26-43 and 6-60 was shown to be activated by the presence of methionine and lysine. These results suggest that the OMP synthesis in mutant D-30 was not completely blocked. Possible mechanisms for the activation of the OMP biosynthesis by methionine and lysine are discussed.View full abstractDownload PDF (1002K) -
Masako YAMADAArticle type: Article
1981 Volume 55 Issue 1 Pages 23-29
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
JOURNAL FREE ACCESSA cup-plate technique for the microbioassay of folate using Lactobacillus casei as a test organism was attempted. The folate content of powdered agar added to basal medium affected the clearness and sharpness of the growth zones. There was a linear relation between the diameters of growth zones and logarithms of the concentrations within the range of 10〜400 ng/ml of pure folate solution. In the statistical evaluation of the assay results the maximum errors of. thc estimate were below 10%. The total folate contents in some natural products obtained by cup-plate method were a little high to those obtained with turbidimetric one and the recovery experiments were successful enough. These resuls suggest that the cup-plate method may be available for the microbiological assay of folate in natural products.View full abstractDownload PDF (902K) -
Masaru TSUJIMURA, Tomoko FUKUDA, Takamasa KASAI, Sasaburo KITAMURAArticle type: Article
1981 Volume 55 Issue 1 Pages 31-35
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
JOURNAL FREE ACCESSThe antiscorbutic activity of 5,6-O-isopropylidene ascorbic acid (IAsA) and 5,6-O-isopropylidene ascorbic acid 2-sulfate (IAsS) in Hartley guinea pig fed a scorbutigenic diet has been investigated. Oral administration (0.1 mmol/animal) of IAsA completely prevented development of scurvy and continued to increase in body weight throughout the feeding experiment in guinea pig. An equimolar dose of IAsS did not prevent scurvy, and total vitamin C levels in tissues of IAsS-administrated animals were similar to those in C-free animals. The vitamin C Levels (μmol/ml or g) of IAsA, ascorbic acid (ASA), IAsS, and vitamin C deficient groups after the feeding test (IAsA, ASA group : 40 days, IAsS, C deficient group: 20 days) were 0.14, 0.11, 0.01, and 0.01 in blood, 4.90, 3.97, 0.07, and 0.05 in liver, 1.89, 1.47, 0.08, and 0.02 in kidney, respectively.View full abstractDownload PDF (505K) -
Kazuko YAMADA, Toyoyuki KATAGI, Hiroshi KUMAOKAArticle type: Article
1981 Volume 55 Issue 1 Pages 37-42
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
JOURNAL FREE ACCESSIt has been proposed by several groups of investigators that the pyrimidine moiety of thiamin is synthesized via 5-aminoimidazole ribotide, an intermediate of purine biosynthetic route in bacteria. To decide whether the methyl group attached to C-2 atom of the pyrimidine is introduced after the recyclization of the imidazole ring of 5-amino-imidazole ribotide (AIR) to the pyrimidine, the competition of radioactive formate incorporation into the pyrimidine moiety by 4-amino-5-hydroxy-methylpyrimidine was investigated. The results showed that this compound was not used as a biosynthetic intermediate by Escherichia coli and Saccharomyces cerevisiae. It remains unsolved, at which step methyl group is introduced and which compound is methyl origin of the pyrimidine moiety of thiamin.View full abstractDownload PDF (633K) -
[in Japanese], [in Japanese]Article type: Article
1981 Volume 55 Issue 1 Pages 43-
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
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[in Japanese], [in Japanese], [in Japanese]Article type: Article
1981 Volume 55 Issue 1 Pages 43-44
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1981 Volume 55 Issue 1 Pages 44-45
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
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[in Japanese], [in Japanese], [in Japanese]Article type: Article
1981 Volume 55 Issue 1 Pages 45-46
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
JOURNAL FREE ACCESSDownload PDF (281K) -
[in Japanese]Article type: Article
1981 Volume 55 Issue 1 Pages 46-
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
JOURNAL FREE ACCESSDownload PDF (137K) -
[in Japanese], [in Japanese]Article type: Article
1981 Volume 55 Issue 1 Pages 47-
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
JOURNAL FREE ACCESSDownload PDF (166K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1981 Volume 55 Issue 1 Pages 47-48
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
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[in Japanese], [in Japanese], [in Japanese]Article type: Article
1981 Volume 55 Issue 1 Pages 48-49
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
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[in Japanese], [in Japanese], [in Japanese]Article type: Article
1981 Volume 55 Issue 1 Pages 49-
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
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[in Japanese], J.R. CooperArticle type: Article
1981 Volume 55 Issue 1 Pages 49-50
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
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[in Japanese], [in Japanese]Article type: Article
1981 Volume 55 Issue 1 Pages 50-51
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
JOURNAL FREE ACCESSDownload PDF (332K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1981 Volume 55 Issue 1 Pages 51-
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
JOURNAL FREE ACCESSDownload PDF (193K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1981 Volume 55 Issue 1 Pages 51-52
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
JOURNAL FREE ACCESSDownload PDF (333K) -
[in Japanese], [in Japanese]Article type: Article
1981 Volume 55 Issue 1 Pages 52-53
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
JOURNAL FREE ACCESSDownload PDF (319K) -
[in Japanese], R.H. AbelesArticle type: Article
1981 Volume 55 Issue 1 Pages 53-
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
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[in Japanese], [in Japanese]Article type: Article
1981 Volume 55 Issue 1 Pages 53-54
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
JOURNAL FREE ACCESSDownload PDF (253K) -
[in Japanese]Article type: Article
1981 Volume 55 Issue 1 Pages 54-
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
JOURNAL FREE ACCESSDownload PDF (119K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1981 Volume 55 Issue 1 Pages 55-
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
JOURNAL FREE ACCESSDownload PDF (168K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1981 Volume 55 Issue 1 Pages 55-56
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
JOURNAL FREE ACCESSDownload PDF (268K) -
[in Japanese], [in Japanese]Article type: Article
1981 Volume 55 Issue 1 Pages 56-
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
JOURNAL FREE ACCESSDownload PDF (148K) -
[in Japanese]Article type: Article
1981 Volume 55 Issue 1 Pages 57-58
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
JOURNAL FREE ACCESSDownload PDF (324K) -
[in Japanese]Article type: Article
1981 Volume 55 Issue 1 Pages 58-60
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
JOURNAL FREE ACCESSDownload PDF (391K) -
[in Japanese]Article type: Article
1981 Volume 55 Issue 1 Pages 60-61
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
JOURNAL FREE ACCESSDownload PDF (172K) -
[in Japanese]Article type: Article
1981 Volume 55 Issue 1 Pages 62-65
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
JOURNAL FREE ACCESSDownload PDF (273K) -
[in Japanese]Article type: Article
1981 Volume 55 Issue 1 Pages 66-
Published: January 25, 1981
Released on J-STAGE: March 09, 2018
JOURNAL FREE ACCESSDownload PDF (124K)
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