-
Yoshitaka NAKAMURA
Article type: Article
1966 Volume 33 Issue 2 Pages
115-121
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
The effect of vitamin K_1 on the endocrine activity was studied in the Wistar-strain rats weighed 120-160g by measuring of urinary 17-KS and the following results were obtained. An increase of the excretion of 17-KS was observed in the rat administered with 100 μg of vitamin K_1 daily for 20 days. The excretion of 17-KS increased temporarily by the administration of 1 mg and 10 mg of vitamin K_1 and, then, it decreased by the continuous administration. These findings suggest that a small dose of vitamin K_1 canses an of increase the activities of adrenal cortex and ovaries, while a large dose induces a transient hyperfunction of the adrenal cortex and ovaries and, then, gradual decrease occurs.
View full abstract
-
Yoshitaka NAKAMURA
Article type: Article
1966 Volume 33 Issue 2 Pages
122-126
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
The effect of vitamin A on the urinary excretion of 17-KS in rat was studied. The determination of the urinary excretion of 17-KS was carried out by the modified method of Drekter, twice a week. No significant change in the excretion of 17-KS was found in the groups which were injected intramuscularly with 100,500 and 1,000 I.U. of vitamin A daily. These findings suggest that vitamin A have no significant effect on the function of adrenal cortex and ovaries.
View full abstract
-
Yoshitaka NAKAMURA
Article type: Article
1966 Volume 33 Issue 2 Pages
127-131
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
The effect of vitamin D on the urinary excretion of 17-KS in rat was studied. Urinary 17-KS was determined by the modified method of Drekter, twice a week. Normal and castrated mature female rats weighed 120-160g were injected intramuscularly with vitamin D for 20 days. No significant change of 17-KS excretion was found in the groups which were administered daily 10 or 100 I.U. of vitamin D, while a tendency of gradual decrease was observed in the animals administered with 1,000 I.U. of vitamin D daily. These findings suggest that no significant change of function of adrenal cortex and ovaries is induced by the administration of a small amount of vitamin D and, on the other hand, a large dose of vitamin D suppresses their functions.
View full abstract
-
Hisayuki FUKUTOMI
Article type: Article
1966 Volume 33 Issue 2 Pages
132-139
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
In the thiamine-deficient rats, body weight and appetite dcereased gradually from the second week of thiamine deficiency. The growth stopped when their body weight reached to 80-90g. In the 4th week anorexia and the body weight decrease developed rapidly. Most of rats lost their vitality and revealed paralytic gastrointestinal symptom. But when thiamine in dose of 40μg was given subcutaneously in the end stage of thiamine deficiency, the rats caused an increase in appetite and body weight on the next day and were cured the paralytic symptom in a few days. They gained 5-10g of weight daily but stopped growing after 3 days and they lost their body weight and became enervated again. After the effect of thiamine lasted for 12-14 days, they died of thiamine deficiency. When 200μg of thiamine was given by subcutaneous injection to thiamine-deficient rats in the 4th week, they regained vitality after 24 hours and their body weight and appetite increased. They gained 8-13g of weight daily and kept growing evenly to 80-90g and stopped growing after 10 days. At the 25 days after injection, deficient syndrome appeared completely, and the animal died in the 4th or 5th week after injection. Effect of thiamine injection in dose of 200μg lasted for 25 days. These results support the view that the excessive administration is apposite for the treatment of thiamin-deficient diseases. In the second experiments, it was found that the growth curves were similar, even if deficiency and recovery by thiamine administration of 200μg were repeated several times. But in case of deficiency and recovery by 50μg thiamine was repeated several times, the rats could not regained their vitality and at last they died in spite of thiamine administration. These results suggest that excessive administration of thiamine is more effective than small administration.
View full abstract
-
Hisayuki FUKUTOMI
Article type: Article
1966 Volume 33 Issue 2 Pages
140-143
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
The author at first divided the rats into 2 groups, one a thiamine deficient group, giving the normal basal diet containing 20% casein and the other thiamine-protein deficient groups, giving the low protein diet with 5% casein. The growth in low protein group stopped after about 5 days, and in the third week, body weight was lowered almost to the initial one. However, the animals did not show any remakable thiamine deficient symptom, as marked neuroparalysis. Most of the rats died earlier than the adequate protein group. In spite of thiamine administration, body weight and appetite in low protein group did not increase. Effects of thiamine administration was not recognized for hypoprotein-thiamine-deficient rats.
View full abstract
-
Hisayuki FUKUTOMI
Article type: Article
1966 Volume 33 Issue 2 Pages
144-150
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
Thiamine contents in the organs of the normal rats after an administration of thiamine derivatives, such as TPD, TTFD, CAT, were examined. In blood, liver and kidney, total thiamine and cocarboxylase contents increased remarkably by the injection of TPD, TTFD or CAT as compared with the injection of thiamine hydrochloride. In the case of oral administration, urinary thiamine excretion by the administration of TPD, TTFD, CAT showed an extremely high value than that by thiamine hydrochloride administration. Clinical effect of thiamine derivatives was remarkable, especially for beri-beri and neuritis. These thiamine derivatives have a superiority over thiamine hydrochloride on affinity to the tissues and maintenance of high thiamine value of tissues.
View full abstract
-
Hisayuki FUKUTOMI
Article type: Article
1966 Volume 33 Issue 2 Pages
151-155
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
Studies were made on the distribution of thiamine in body of rat, its excretion in urine and faeces, and the amount of thiamine decomposed in the body after an excessive administration of thiamine. When 500μg of thiamine hydrochloride per 100g body weight was given subcutaneously for 20 days to young rat, total excreted thiamine was 60% of given dose and the amount decomposed in the body was calculated as 36%. In 24 hours experiment, the elimination and destruction rates were 48.5 and 25.8%, respectively. In the case of 500μg per 100g of thiamine propyldisulfide administration their values were 35 and 25%. It was recognized that the retention of thiamine was lowered by the carbon tetrachloride hepatic injury.
View full abstract
-
Hisayuki FUKUTOMI
Article type: Article
1966 Volume 33 Issue 2 Pages
156-159
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
Recently, there is a tendency to use successively of a large amount of thiamine. The author studied whether an administration of excessive amount of thiamine have ill effect or not. No harmful effect was observed when 0.5-1mg of thiamine was injected subcutaneously for 90 days on rat. However, an administration of 0.5mg of thiamine for pyridoxine-deficient rat caused somewhat depression of body weight, compared with that of pyridoxine deficiency without thiamine. It was also revealed that a large amount of thiamine accelerates the running fit caused by oxymethylpyrimidine for rat or mouse. These results suggest that excess dosage of thiamine promotes the pyridoxine deficiency.
View full abstract
-
Hisayuki FUKUTOMI
Article type: Article
1966 Volume 33 Issue 2 Pages
160-162
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
When electric shock was given for rat, 3 times at intervals of 2 hours, the animals were necropsied at the end of the 3rd convulsion. Thiamine content of their organs decreased and pyruvic acid content increased by electrical shock as well as in fit by oxymethylpyrimidine.
View full abstract
-
Hisayuki FUKUTOMI
Article type: Article
1966 Volume 33 Issue 2 Pages
163-165
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
Thiamine content of rat organs did not increase, even if thiochrome was administered orally, subcutaneously or interavenously, and also thiamine deficiency was not recovered.
View full abstract
-
Mitsuo KAMIMURA
Article type: Article
1966 Volume 33 Issue 2 Pages
166-173
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
Experimental studies of the vitamin E on the skin microcirculation were done, especially on the capillary resistance, capillary permeability, skin temperature and skin blood volume, mainly on the human skin. Discussing the results it was concluded that vitamin E had the accelerating and normalising effects on the arteries and venous microcirculation, especially at the peripheral region.
View full abstract
-
Kiyoshi TSUKIDA, Miya YOKOTA, Keiko IKEUCHI
Article type: Article
1966 Volume 33 Issue 2 Pages
174-178
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
All-trans β-carotene in various solvents was irradiated with W-, Xe-, Hg-lamp or sunlight and the decreasing ratio of the light absorbance at λ_<mxa> was preliminary estimated. Irradiation products of β-carotene in n-hexane, acetone, chloroform or carbon tetrachloride were evaluated by column chromatography and were determined spectrometrically. They were identified as retro-dehydrocarotenes (all-trans, neo-A, D and F), mutatochromes (all-trans and neo-A) and β-carotenes (recovered all-trans, neo-U, B and E). Experimental data obtained under various conditions indicate that β-carotene in the above solvents does not seem to be epoxidized by short insolation.
View full abstract
-
Kiyoshi TSUKIDA, Miya YOKOTA, Keiko IKEUCHI
Article type: Article
1966 Volume 33 Issue 2 Pages
179-184
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
Bubbling the dried air through the solution of all-trans β-carotene (I) in n-hexane, acetone or chloroform (irradiated with Xe-lamp or in dark), followed by column chromatography revealed the formation of the complicated mixture of oxidation products. They were identified and determined as all-trans and cis compounds of zeaxanthin, cryptoflavin, retro-dehydrocarotene, cryptoxanthin, I-5,8-monoepoxide (mutatochrome), I and I-5,6-monoepoxide in order of decreasing adsorption on the column. The yield of mutatochrome was amounted to 20% (15% of all-trans and 5% of cis) when I was air-oxidized in acetone solution in the presence of 3.4 NH_2SO_4. The efficacy of adding an acid for the formation of some epoxycarotenes was also observed in the liquid phase autoxidation of I. Abnormal formation of 3,4- and 3,4,3', 4'-dehydro-I and oxidation mechanism were also discussed.
View full abstract
-
Kiyoshi TSUKIDA
Article type: Article
1966 Volume 33 Issue 2 Pages
185-191
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
Three of the title compounds, canthaxanthin (I), echinenone (II) and isozeaxanthin (III), were oxidized with perphthalic acid or H_2O_2 and the products were evaluated spectroscopically. I gave no epoxides in either method and was mostly recovered together with small amounts of neo-I A-F and unidentified pigments. II was oxidized with perphthalic acid to give 5', 6'-monoepoxide (IV), mp. 179-180℃, accompanied by considerable amounts of corresponding cis and 3', 4'-dehydro-II, while II-5,6-mono- or 5,6,5', 6'-diepoxide was not obtained. III was smoothly epoxidized with perphthalic acid to give its 5,6-monoepoxide (V), mp. 177-178℃, and 5,6,5', 6'-diepoxide (VI), mp. 203-204℃, together with small amounts of 5,6,5', 8'-diepoxide. On treating V and VI with chloroform containing HCl gas, they were transformed to III-5,8-mono- and 5,8,5', 8'-diepoxide, respectively, although IV gave 5'-hydroxy-5', 6'-dihydro-II.
View full abstract
-
Yoshishige SATO, Mari SAKUMA, Tadao TAKAHASHI
Article type: Article
1966 Volume 33 Issue 2 Pages
192-195
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
The comparative study of the distribution of orally administered O-benzoylthiamine disulfide-^<35>S (BTDS-^<35>S) and thiamine-^<35>S has been carried out in baby and adult mice by means of whole body autoradiographic methods. The autoradiogram revealed that the uptake of ^<35>S was higher and more rapid in the liver, the heart, the lung and the other tissues at short intervals (30-60 mins.) when BTDS-^<35>S was administered than when thiamine-^<35>S was given. This suggests that BTDS is more readily absorbed from digestive tract than thiamine.
View full abstract
-
[in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
196-
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
196-197
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
197-
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
197-198
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
198-
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
198-
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
198-199
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
199-
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
200-
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
200-201
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
201-
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
202-
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
202-203
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
203-204
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
204-
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
204-
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
204-205
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
205-
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
205-206
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
206-
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
206-207
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
207-
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
207-208
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
208-
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
208-
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
208-209
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
209-210
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
210-
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese], [in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
210-211
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
211-212
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
212-
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
212-213
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
213-
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS
-
[in Japanese], [in Japanese], [in Japanese]
Article type: Article
1966 Volume 33 Issue 2 Pages
213-
Published: February 25, 1966
Released on J-STAGE: February 09, 2018
JOURNAL
FREE ACCESS