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Volume 27 (1963)
- Issue 6 Pages 423-
- Issue 5 Pages 331-
- Issue 4 Pages 251-
- Issue 3 Pages 163-
- Issue 2 Pages 97-
- Issue 1 Pages 1-
Volume 27, Issue 6
Displaying 1-41 of 41 articles from this issue
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Chikataro KAWASAKI, Chisae YAMADAArticle type: Article
1963 Volume 27 Issue 6 Pages 423-432
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
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Toru ADACHIArticle type: Article
1963 Volume 27 Issue 6 Pages 433-437
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSA colorimetric determination of orotic acid reported by Tsuji which consists of three steps, bromination of orotic acid, reduction by thioglycollate and condensation with p-dimethylaminobenzaldehyde to yield an orange color, was examined in detail and improved in several points. Ascorbic acid gave better results than thioglycollate in the step of reduction. Satisfactory results were obtained when the improved method was applied to several multivitamin preparations.View full abstractDownload PDF (520K) -
Toru ADACHIArticle type: Article
1963 Volume 27 Issue 6 Pages 438-441
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSThe improved p-dimethylaminobenzaldehyde method for colorimetric determination of orotic acid was reported in the previous report. This paper describes the mechanism of the color development. It was elucidated that bromination of orotic acid results in the production of 5,5-dibromobarbituric acid and the brominated compound is reduced with ascorbic acid to barbituric acid which condenses with p-dimethylaminobenzaldehyde to yield an orange color, 5-(p-dimehytlaminobenzylidene)-barbituric acid.View full abstractDownload PDF (412K) -
Yutaka TOMODAArticle type: Article
1963 Volume 27 Issue 6 Pages 442-446
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSTo study on the effect of vitamin E on blood coagulation in vivo, 300mg of vitamin E was given by intramuscular injection to rabbits for 9 days and changes of blood coagulation factors were investigated. Blood coagulation time, recalcified clotting time and thrombin time were prolonged, but the first and the second stage of blood coagulation showed no change. Plasmin and whole plasmin value were elevated. These results show that vitamin E is antithrombic and activated fibrinolysis in vivo. It is in agreement with my result of experiment in vitro.View full abstractDownload PDF (600K) -
Kyoichi KOBASHIArticle type: Article
1963 Volume 27 Issue 6 Pages 447-450
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSDuring the course of experiment on the nature of crystalline urease, bis-(p-nitrophenyl) disulfide (I) was found to be a potent inhibitor of urease activity. It was shown that (I) was more inhibitory at an alkaline medium of higher pH and the inhibition was progressive with time. The inhibition of urease was prevented or recovered by the addition of an excess amount of such sulfhydryl compounds as reduced glutathione and cysteine. Possible mechanism of inhibition by bis-(p-nitrophenyl) disulfide seems to be due to disulfide-exchange reactions between the inhibitor and sulfhydryl groups of urease with a progressive loss of its activity.View full abstractDownload PDF (444K) -
Kyoichi KOBASHIArticle type: Article
1963 Volume 27 Issue 6 Pages 451-454
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSAmong the thiamine derivatives tested, thiamine disulfide (TDS), O-benzoylthiamine disulfide and O, S-diacetylthiamine showed to be definitely inhibitory to urease activity. On the other hand, O, S-dibenzoylthiamine, O, S-dicarbethoxythiamine and thiamine had no inhibitory action. These inhibitions were observed at the alkaline pH and progressive with time, and could be prevented or recovered by the addition of an excess amount of sulfhydryl compounds. The reaction of TDS with crystalline urease produced thiamine bound urease with loss of its ureolytic activity, from which thiamine was liberated by sulfhydryl compounds. Therefore the mechanism of inhibition of urease activity by the disulfide compounds seems to be due to disulfide-exchange reactions between the S-S group of TDS and the SH groups of urease.View full abstractDownload PDF (479K) -
Masuyuki KATSUMATA, Eiichi SAKAKIBARA, Keiko TSUJIMOTOArticle type: Article
1963 Volume 27 Issue 6 Pages 455-460
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSIt was been reported that thiaminase II catalizes the second-order reaction. The general kinetics of the reaction, A+B→products, were therefore studied. The basic rate-equation in this study was that intoduced by Dixon and Webb, in which almost no enzymatic reaction was assumed. Though the equation was very complicated, it seemed pertinent to explain actual enzymatic reactions. Applications of this equation from its theoretical basis were discussed in this paper. The second-order enzymatic reactions were classified into the following three types : linear type, substrate-activating type and substrate-inhibitory type. These types are resulted from the modes of steady state of the reaction. In "linear steady state", EAB ternary complex is formed through EA and EB at random sequence. The reaction follows Michaelis-like relation. The types showing phenomena of both substrate-activation and substrate-inhibition are caused by "cyclic steady state". Assuming a cyclic sequence of enzymesubstrate-complex-formation in steady state as E→EA→EAB→EB→(E), the equation suggests that the reaction is activated by substrate A and is inhibited by substrate B. Methods for the determination of both the maximum velocity and the dissociating constant of EAB ternary complex were also discussed.View full abstractDownload PDF (666K) -
Ichiro CHIBATA, Shigeki YAMADA, Hiroshi ITO, Setsuko ISHIKAWAArticle type: Article
1963 Volume 27 Issue 6 Pages 461-465
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSRecently, Weeks and Beck revealed that Flavobacterium aquatile strain Taylor responded specifically to thiamine in a simple medium and applied this organism for the assay of thiamine. In present work, growth response of the organism for thiamine was studied under varied conditions. As a result, it was confirmed that the growth of this organism was proportional to thiamine hydrochloride in the range of 0〜4.0mμg/ml when culture was carried out for 47 hours at 24℃ and pH 7.5 in the medium containing Trypticase 0.5%, glucose 0.5%, K_2HPO_4 0.05%, KH_2PO_4 0.05% and MgSO_4・7H_2O 0.02%. Under these conditions, microbiological activity of thiamine diphosphate was approximately equal to that of thiamine hydrochloride, and the activity of thiamine disulfide was somewhat inferior. On the other hand, O-benzoylthiamine, O-benzoylthiamine disulfide, and pyrimidine and thiazole moieties of thiamine were inactive. Practical application for the assay of thiamine in medicines and enriched foods was also investigated. The results were satisfactory and the values obtained were in good agreement with those assayed by thiochrome method.View full abstractDownload PDF (570K) -
[in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 466-
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
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[in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 466-467
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
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[in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 467-468
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
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[in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 468-
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSDownload PDF (217K) -
[in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 468-
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSDownload PDF (217K) -
[in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 469-
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
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[in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 469-
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSDownload PDF (202K) -
[in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 469-470
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
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[in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 470-471
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
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[in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 471-472
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSDownload PDF (348K) -
[in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 472-
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
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[in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 472-473
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
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[in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 473-
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
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[in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 473-474
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
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[in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 474-475
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
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[in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 475-
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
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[in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 475-
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
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[in Japanese], [in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 476-
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSDownload PDF (172K) -
[in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 476-
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
JOURNAL FREE ACCESSDownload PDF (172K) -
[in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 476-477
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
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[in Japanese], [in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 477-
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
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[in Japanese], [in Japanese], [in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 477-
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
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[in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 477-478
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
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[in Japanese], [in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 478-
Published: June 25, 1963
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[in Japanese], [in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 478-479
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
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[in Japanese], [in Japanese], [in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 479-
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
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[in Japanese], [in Japanese], [in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 479-
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
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[in Japanese], [in Japanese], [in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 479-480
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
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[in Japanese], [in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 480-
Published: June 25, 1963
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[in Japanese], [in Japanese], [in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 480-481
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
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[in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 481-
Published: June 25, 1963
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[in Japanese], [in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 481-
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
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[in Japanese]Article type: Article
1963 Volume 27 Issue 6 Pages 483-
Published: June 25, 1963
Released on J-STAGE: January 27, 2018
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