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Volume 55, Issue 9-10
Displaying 1-19 of 19 articles from this issue
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Haruhito TSUGE, Junko SAKUMA, Kenji OZEKI, Kazuji OHASHIArticle type: Article
1981 Volume 55 Issue 9-10 Pages 437-443
Published: October 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSEnzymatic properties of immobilized pyridoxaminephosphate oxidase (EC 1. 4. 3. 5) and the continuous production of pyridoxal 5'-phosphate (PLP) by the immobilized enzyme column were studied The enzyme partially purified from dry baker's yeast was immobilized to agarose matrix via phenyl-mercury ligand, and then lyophilized. The relative rate of activity for pyridoxine 5'-phosphate (PNP) to that for pyridoxamine 5'-phosphate (PMP) apparently increased (ratio of PNP oxidase activity per PMP oxidase activity was more than 1), where biphasic Lineweaver-Burk plots for two substrates were revealed, indicating that the immobilized, lyophilized preparation could efficiently oxidize PNP and PMP at high concentration. By combining the immobilized enzyme and aminoethyl-Sephadex columns, the sufficient production of PLP and its removal from the solution were achieved, when the solution (100 ml, 10 nmol substrate) was recycled in a closed-like system. β-Alanine-NaOH buffer (0.2 M, pH 8.0 or 9.0) was sufficient to allow the continuous production of PLP (conversion ratio; 80%) as well as its removal by adding substrate and by adjusting pH for several cycles.View full abstractDownload PDF (693K) -
Teruo FUKUDA, Kimie KOBAYASHIArticle type: Article
1981 Volume 55 Issue 9-10 Pages 445-451
Published: October 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSThe effect of sodium chloride on the stability of thiamine in the presence of Cu^<2+> was investigated and following results were obtained. 1. Sodium chloride decomposed thiamine in the reaction mixture of thiamine 0.9 mg% and Cu^<2+> 32 μg% at 90℃, pH 7.0. 2. Formation of thiamine disulfide (TDS) was in very small amounts in the investigation on the effect of Cu^<2+> concentration, heating time, heating temperature and pH. 3. The action of sodium chloride was dependent upon the coexistence of Na^+ and Cl^-. In the case of cations, NH_4^+ also has a considerable effect as K^+ and Na^+ and in the case of anions, I^- and Br^- has also a considerable effect as Cl^-. 4. Thiochrome, TDS, 2-methyl-4-amino-5-hydroxymethylpyrimidine, 4-methyl-5-β-hydroxymethylthiazole and 2-methyl-4-amino-5-aminomethylpyrimidine were detected as the decomposition products.View full abstractDownload PDF (596K) -
Takako OGAWA, Hiroshi HIRANO, Takahiro NISHIMUNE, Ryoji HAYASHIArticle type: Article
1981 Volume 55 Issue 9-10 Pages 453-459
Published: October 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSIt has been found that the oxidation of thiamine by cyanogen bromide (BrCN) under alkaline condition produces a new fluorescent compound, O-carbamoylthiochrome (Carb-Thc), as well as thiochrome (Thc). The structure of Carb-Thc was determined by its spectral data and comparison with an authentic sample of this compound derived from O-carbamoylthiamine. Carb-Thc was characterized from the UV spectrum and fluorescence spectrum in comparison with those of Thc. The spectroscopic features of both compounds were essentially identical. These observations suggest that assay of thiamine by BrCN-NaOH method is not disturbed by contamination of Carb-Thc which may be formed as a by-product in the assay process. Amounts of Thc and Carb-Thc produced under several reaction conditions were determined.View full abstractDownload PDF (649K) -
Tadashi KOBAYASHIArticle type: Article
1981 Volume 55 Issue 9-10 Pages 461-468
Published: October 25, 1981
Released on J-STAGE: March 10, 2018
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Article type: Appendix
1981 Volume 55 Issue 9-10 Pages 469-471
Published: October 25, 1981
Released on J-STAGE: March 10, 2018
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[in Japanese]Article type: Article
1981 Volume 55 Issue 9-10 Pages 472-
Published: October 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSDownload PDF (193K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1981 Volume 55 Issue 9-10 Pages 473-
Published: October 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSDownload PDF (159K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
1981 Volume 55 Issue 9-10 Pages 473-474
Published: October 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSDownload PDF (294K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
1981 Volume 55 Issue 9-10 Pages 474-475
Published: October 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSDownload PDF (326K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
1981 Volume 55 Issue 9-10 Pages 475-
Published: October 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSDownload PDF (196K) -
[in Japanese]Article type: Article
1981 Volume 55 Issue 9-10 Pages 475-476
Published: October 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSDownload PDF (228K) -
[in Japanese], [in Japanese]Article type: Article
1981 Volume 55 Issue 9-10 Pages 477-
Published: October 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSDownload PDF (173K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1981 Volume 55 Issue 9-10 Pages 478-
Published: October 25, 1981
Released on J-STAGE: March 10, 2018
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[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...Article type: Article
1981 Volume 55 Issue 9-10 Pages 478-479
Published: October 25, 1981
Released on J-STAGE: March 10, 2018
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[in Japanese]Article type: Article
1981 Volume 55 Issue 9-10 Pages 479-480
Published: October 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSDownload PDF (290K) -
[in Japanese], [in Japanese], [in Japanese]Article type: Article
1981 Volume 55 Issue 9-10 Pages 480-
Published: October 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSDownload PDF (147K) -
[in Japanese]Article type: Article
1981 Volume 55 Issue 9-10 Pages 481-
Published: October 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSDownload PDF (140K) -
[in Japanese], [in Japanese]Article type: Article
1981 Volume 55 Issue 9-10 Pages 481-483
Published: October 25, 1981
Released on J-STAGE: March 10, 2018
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[in Japanese]Article type: Article
1981 Volume 55 Issue 9-10 Pages 483-484
Published: October 25, 1981
Released on J-STAGE: March 10, 2018
JOURNAL FREE ACCESSDownload PDF (291K)
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