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Volume 74, Issue 8
Displaying 1-13 of 13 articles from this issue
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[in Japanese]Article type: Article
2000 Volume 74 Issue 8 Pages 409-
Published: August 25, 2000
Released on J-STAGE: December 26, 2017
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Kyoko NAKANO, Shigeo OHTA, Shiro NAKAGAWA, Sadayuki MATUDAArticle type: Article
2000 Volume 74 Issue 8 Pages 411-422
Published: August 25, 2000
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSA family of α-ketoacid dehydrogenase complex includes α-ketoglutarate dehydrogenase (α-KGDC), pyruvate dehydrogenase and branched chain α-ketoacid dehydrogenase complexes. These three complexes are composed of three different component enzymes, α-ketoacid decarboxylase (E1), dihydrolipoamide acyltransferase (E2) and dihydrolipoamide dehydrogenase (E3). We isolated and sequenced cDNA clones for the dihydrolipoamide succinyltransferase (E2 of α-KGDC, designated as DLST) components of the rat and human α-KGDCs. The primary structures of the rat and human DLSTs showed close similarity to those of E. coll and A. vinelandii DLSTs. However, theratandhumanDLSTsdidnotcontainasequencemotifthathadbeenfoundasanE3 and/or E1 binding domain in the E2 components of three α-ketoacid dehydrogenase complexes, suggesting the lack of the E3 and/or E1 binding domain in the rat and human DLSTs. The result of phylogenetic tree of the E2 components of three complexes demonstrated that the lack of the domain in rat and human DLSTs might occur after the mitochondrial symbiosis. Next, we isolated genomic DNA and processed pseudogene of human DLST and determined their entire nucleotide sequences. In situ hybridization analysis demonstrated that the human DLST gene is located on chromosome 14 at q24.2-q24.3 and the pseudogene is located on chromosome 1 at p31. The results of CAT and gel shift assays showed that another protein rather than Sp1 plays a significant role with Ap2 in the transcription-regulation of DLST gene. In addition, we detected polymorphisms in the human DLST gene and found an association of a genotype (ac/ac type) of DLST gene with Alzheimer's disease. Furthermore, we have observed that in the skeletal muscle the DLST is also localized on the plasma membrane and sarcoplasmic reticulum and that the molecule (25 kDa) is smaller than mitochondrial DLST (48 kDa).View full abstractDownload PDF (1370K) -
Keiko SHIBATA, Yasuyo YASUHARA, Kazuto YASUDAArticle type: Article
2000 Volume 74 Issue 8 Pages 423-433
Published: August 25, 2000
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSTo study losses of vitamin B_6 (B_6) in foods by cooking, the B_6 contents in foods and the B_6 retention ratios were measured by HPLC before and after various cooking. Further, the retention of B_6 in a day's meal of female students was reconfirmed to estimate approximate cooking loss of B_6. Ten animal foods and six plant foods were cooked by three methods (sauteeing, deep-frying and boiling) basically, and the B_6 vitamers (PMP, PM, PLP, PL, PNP, PN and PNG) in foods were measured by HPLC. The results are as follows, (1) The dry heating methods had an advantage over the wet heating methods in retention ratio of B_6, which were 60〜80 % for meats and 95 % for potato. (2) The retention ratios on the wet heating methods were 30〜50 % for animal foods and this methods had the higher retentions at the short time of heating. The ratios for plant foods were 40〜75 %, because of B_6 in plant foods were more stable. (3) The difference of the retention ratios between the animal foods and plant foods suggested that the ratios depended on the compositions of B_6 vitamers. (4) The cooking loss of B_6 was considered to be approximately 20 % in daily meals which were included non-heated foods in home cooking and used processed foods in parts (40 %) of the meals, from the result of this study on female students of today.View full abstractDownload PDF (1092K) -
Kazuko OBA, Atsuko YAMAMOTO, Azusa KAWAI, Ayumi SAKATA, Mahoyo YAMAZAK ...Article type: Article
2000 Volume 74 Issue 8 Pages 435-440
Published: August 25, 2000
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSWe tried to clarify whether the total vitamin C (ascorbic acid plus dehydro-ascorbic acid) content in fresh, frozen and cooked vegetables from commercial sources measured depends on the method used for measurement. Total vitammin C content in fresh vegetables measured by the colorimetric method using 2, 4-dinitrophenylhydrazine was similar to that obtained by HPLC, and consistent with the values indicated in the standard table of Food Composition in Japan. However, total vitamin C content in frozen and cooked vegetables measured by HPLC was only 47.5 % of that measured by the colorimetric method. These data indicate that the actual value of vitamin C content in cooked vegetables is one third to one fifth that in fresh vegetables.View full abstractDownload PDF (561K) -
Kazuki OKAMOTO, Fumihide ISOHASHIArticle type: Article
2000 Volume 74 Issue 8 Pages 441-443
Published: August 25, 2000
Released on J-STAGE: December 26, 2017
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Keiko YAMADA, Yoko YAMADA, Morimichi FUKUDA, Shoji YAMADAArticle type: Article
2000 Volume 74 Issue 8 Pages 445-446
Published: August 25, 2000
Released on J-STAGE: December 26, 2017
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[in Japanese], [in Japanese], [in Japanese]Article type: Article
2000 Volume 74 Issue 8 Pages 447-449
Published: August 25, 2000
Released on J-STAGE: December 26, 2017
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[in Japanese]Article type: Article
2000 Volume 74 Issue 8 Pages 449-450
Published: August 25, 2000
Released on J-STAGE: December 26, 2017
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Article type: Bibliography
2000 Volume 74 Issue 8 Pages 451-453
Published: August 25, 2000
Released on J-STAGE: December 26, 2017
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[in Japanese], [in Japanese]Article type: Article
2000 Volume 74 Issue 8 Pages 455-456
Published: August 25, 2000
Released on J-STAGE: December 26, 2017
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[in Japanese], [in Japanese], [in Japanese], [in Japanese]Article type: Article
2000 Volume 74 Issue 8 Pages 456-457
Published: August 25, 2000
Released on J-STAGE: December 26, 2017
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[in Japanese], [in Japanese], [in Japanese]Article type: Article
2000 Volume 74 Issue 8 Pages 457-
Published: August 25, 2000
Released on J-STAGE: December 26, 2017
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[in Japanese], [in Japanese]Article type: Article
2000 Volume 74 Issue 8 Pages 457-458
Published: August 25, 2000
Released on J-STAGE: December 26, 2017
JOURNAL FREE ACCESSDownload PDF (274K)
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