VITAMINS

Online ISSN : 2424-080X
Print ISSN : 0006-386X

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Volume 49, Issue 7

Displaying 1-50 of 99 articles from this issue

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Studies on Release of Thiamine from Thiamine diphosphate and Thiamine monophosphate by Cell Free Extracts of Lactobacillus fermenti

Masaomi KONDO, Tomio ICHIKAWA, Kazue SANO, Keiji SANO, Yasuo KAKIUCHI, ...

Article type: Article
1975 Volume 49 Issue 7 Pages 251-254
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_251

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It was found that L.fermenti cells have an enzymic activity hydrolyzing thiamine diphosphate (TDP) and thiamine monophosphate (TMP) to thiamine and this activity is specific for TMP and TDP differing from p-nitrophenyl-phosphatase of the cells. The results of studies on activity-pH profile showed two maximum activities at pH 6.0 and 8.0 to TMP and one maximum activity at pH 6.0 to TDP. The activity to TMP is about four times as much as that to TDP.

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Studies on Properties of Phosphatase of Lactobacillus fermenti Specific for Thiamine diphosphate and Thiamine monophosphate

Masaomi KONDO, Tomio ICHIKAWA, Kazue SANO, Keiji SANO, Yasuo KAKIUCHI, ...

Article type: Article
1975 Volume 49 Issue 7 Pages 255-258
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_255

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Properties of phosphatase having specific activity for thiamine diphosphate and thiamine monophosphate were studied. When compared with p-nitrophenylphosphatase of L.fermenti, this phosphatase activity was inhibited by Mg^<2+> and Na_2MoO_4,while the p-nitrophosphatase was activated by Mg^<2+> and not affected by Na_2MoO_4. This enzyme activity was not affected by other phosphate compounds at all. When L.fermenti cells were cultivated with thiamine, the enzyme activity was decreased with increasing amounts of thiamine added to the medium.

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Determination of Tocopherols in Serum by High Speed Liquid Chromatography

Kouichi ABE, Goichiro KATSUI

Article type: Article
1975 Volume 49 Issue 7 Pages 259-263
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_259

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A new high speed liquid chromatographic (HSLC) method for the determination of tocopherols (Toc) in serum is described. The Toc were extracted from 0.5ml of serum with 5.0ml of n-hexane after precipitation of proteins with ethanol. Four ml of the n-hexane extracts was evaporated under N_2 gas at 40℃. The residue was dissolved in 50μl of n-hexane. Twenty μl of the solution was injected into the HSLC column The eluted Toc were determined separa tely by using a fluorometer (Ex.298nm, Em.325nm). Fat-soluble substances other than Toc in serum sometimes interfere the determination by commonly used method, while they did not in this method. It was found that the proposed HSLC method was more simple and accurate than conventional methods for the separative determination of Toc in serum.

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Thiamine Metabolism in the Experimental Magnesium Deficient Rat : (IV) CHANGES IN BODY TEMPERATURE AND SEROTONIN METABOLISM IN MAGNESIUM DEFICIENT RAT

Mieko KIMURA, Yoshinori ITOKAWA

Article type: Article
1975 Volume 49 Issue 7 Pages 265-271
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_265

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To clarify the effect of thiamine on serotonin metabolism in magnesium deficient rats, in vivo and in vitro experiments were carried out. Six different synthetic diets were given to Wistar rats for two or four weeks. Group I, thiamine and magnesium deficient ; group II, thiamine deficient and magnesium sufficient ; group III, thiamine adequate, magnesium deficient ; group IV, thiamine adequate, magnesium sufficient ; group V, thiamine excess, magnesium deficient ; group VI, thiamine excess, magnesium sufficient. After 10 days on these dietary regimens, rats fed a magnesium deficient diet (groups I, III and V) developed erythema in the ears and nose. However, in rats fed both thiamine and magnesium deficient diet (group I) these symptoms were disappeared at the four week period on regimens. Body temperature decreased markedly in thiamine deficient groups of I and II. Among thiamine sufficient groups, body temperatures were lower in magnesium deficient groups (groups III and V) rather than magnesium sufficient groups (groups II and IV). Serotonin levels in blood and liver increased in groups I, III and V after 2 weeks, but after 4 weeks, serotonin decreased to normal value in group I. Serotonin oxidation activity in liver and stomach decreased significantly in groups II and IV. In vitro addition of a metal chelating agents and either thiamine or thiamine pyrophosphate to brain and liver mitochondria of intact rats resulted in a decrease in serotonin oxidation activities.

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[title in Japanese]

P.V. Khandwekar, N. Nath, M.C. Nath

Article type: Article
1975 Volume 49 Issue 7 Pages 273-
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_273_1

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[title in Japanese]

Y. Iwamoto, T. Yamaguchi, K. Folkers

Article type: Article
1975 Volume 49 Issue 7 Pages 273-
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_273_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 273-274
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_273_3

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 274-
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_274_1

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[title in Japanese]

P. Migasena, S. Changbumrung, V. Supawan, J. Limtrakarn

Article type: Article
1975 Volume 49 Issue 7 Pages 274-
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_274_2

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 274-275
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_274_3

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[title in Japanese]

F.P. Schelp, P. Migasena, S. Saovakontha, P. Pongpaew

Article type: Article
1975 Volume 49 Issue 7 Pages 275-
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_275_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 275-
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_275_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 275-
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_275_3

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 277-278
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_277

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 278-
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_278_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 278-279
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_278_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

Article type: Article
1975 Volume 49 Issue 7 Pages 279-280
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_279

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 280-
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_280_1

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 280-281
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_280_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 281-282
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_281

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], Busaba Yongsmith

Article type: Article
1975 Volume 49 Issue 7 Pages 282-
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_282

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 283-
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_283_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

Article type: Article
1975 Volume 49 Issue 7 Pages 283-284
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_283_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 284-
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_284_1

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 284-
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_284_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 284-285
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_284_3

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 285-
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_285_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

Article type: Article
1975 Volume 49 Issue 7 Pages 285-
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_285_2

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[title in Japanese]

[in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 285-286
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_285_3

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 286-
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_286

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[title in Japanese]

[in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 287-
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_287_1

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[title in Japanese]

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1975 Volume 49 Issue 7 Pages 287-288
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_287_2

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 288-
Published: July 25, 1975
Released on J-STAGE: March 06, 2018

DOIhttps://doi.org/10.20632/vso.49.7_288

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 289-
Published: July 25, 1975
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.49.7_289_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 289-290
Published: July 25, 1975
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.49.7_289_2

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 290-
Published: July 25, 1975
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.49.7_290_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 290-291
Published: July 25, 1975
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.49.7_290_2

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 291-
Published: July 25, 1975
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.49.7_291_1

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 291-292
Published: July 25, 1975
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.49.7_291_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 292-
Published: July 25, 1975
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.49.7_292_1

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[in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 292-293
Published: July 25, 1975
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.49.7_292_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 293-
Published: July 25, 1975
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.49.7_293_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 293-
Published: July 25, 1975
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.49.7_293_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 294-
Published: July 25, 1975
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.49.7_294_1

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 294-
Published: July 25, 1975
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.49.7_294_2

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 294-295
Published: July 25, 1975
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.49.7_294_3

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

Article type: Article
1975 Volume 49 Issue 7 Pages 295-
Published: July 25, 1975
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.49.7_295

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[title in Japanese]

[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...

Article type: Article
1975 Volume 49 Issue 7 Pages 296-
Published: July 25, 1975
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.49.7_296_1

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 296-
Published: July 25, 1975
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.49.7_296_2

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[title in Japanese]

[in Japanese], [in Japanese]

Article type: Article
1975 Volume 49 Issue 7 Pages 297-
Published: July 25, 1975
Released on J-STAGE: March 14, 2018

DOIhttps://doi.org/10.20632/vso.49.7_297_1

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